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Dynamics of Progesterone, TNF- α , and a Metabolite of PGF2 α in Blood Plasma of Beef Cows following Embryo Transfer.

Mason MC, Copeland J, Cuadra EJ, Elsasser TH, Jung Y, Larson J - Vet Med Int (2014)

Bottom Line: Blood was collected (every 15 min for 2 h) in half the animals in each treatment group on d 14 and the remaining half on d 21 for analysis of prostaglandin F2α metabolite (PGFM).Progesterone was greater (P ≤ 0.05) in cows receiving hCG compared to others on d 14.Progesterone in all treatment groups increased from d 7 to d 14 and declined (P ≤ 0.05) from d 14 to d 21.

View Article: PubMed Central - PubMed

Affiliation: Department of Agriculture, Alcorn State University, 1000 ASU Drive No. 750, Alcorn State, Lorman, MS 39096, USA.

ABSTRACT
Lactating beef cows previously synchronized for estrus (d 0) were assigned to four treatments to assess their effectiveness in increasing blood progesterone (P4) and its effects on tumor necrosis factor-α (TNF-α) and prostaglandin F2α (PGF2α) after the transfer of embryos. At the time of transfer (d 7), cows received no treatment (control; n = 16), a controlled internal drug releasing device (CIDR; n = 16), human chorionic gonadotropin (hCG; n = 15), or gonadotropin releasing hormone (GnRH; n = 15). Blood samples were taken on d 7, 14, and 21 for analysis of P4 and tumor necrosis factor-α (TNF-α). Blood was collected (every 15 min for 2 h) in half the animals in each treatment group on d 14 and the remaining half on d 21 for analysis of prostaglandin F2α metabolite (PGFM). Retention rates were 56.2, 62.5, 46.7, and 13.3% for cows in the control, CIDR, hCG, and GnRH groups, respectively. Progesterone was greater (P ≤ 0.05) in cows receiving hCG compared to others on d 14. Progesterone in all treatment groups increased from d 7 to d 14 and declined (P ≤ 0.05) from d 14 to d 21. Contrary to pregnant cows, P4 and TNF-α declined from d 7 to d 21 in nonpregnant cows (P ≤ 0.05). Although PGFM increased by d 21, there was no difference between pregnant and nonpregnant cows.

No MeSH data available.


Related in: MedlinePlus

Concentrations (Mean ± SEM) of TNF-α in pregnant and nonpregnant cows on d 7, d 14, and d 21. Pregnancy status x day interaction (P ≤ 0.05); CIDR = controlled internal drug release; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin.
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Related In: Results  -  Collection


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fig4: Concentrations (Mean ± SEM) of TNF-α in pregnant and nonpregnant cows on d 7, d 14, and d 21. Pregnancy status x day interaction (P ≤ 0.05); CIDR = controlled internal drug release; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin.

Mentions: An overall comparison between pregnant and nonpregnant animals (Figure 4) showed a decrease (P ≤ 0.05) in concentration of TNF-α from d 7 to d 21 in nonpregnant cows, which occurred similarly in P4 in this same experimental group. Previously, it has been reported by our laboratory that low concentrations of  TNF-α  are linked to low concentrations of P4 in nonpregnant cows [10]. The nonpregnant group additionally showed a greater (P ≤ 0.05) concentration of TNF-α on d 7 compared to the pregnant group for reasons unable to be determined with these results. However, it is noteworthy that contrary to nonpregnant animals, pregnant cows maintained steadier concentrations of TNF-α through the entire experimental period. Interestingly, TNF-α has been reported having luteolytic properties. Some investigators [46–48] have suggested that TNF-α is deleterious to young embryos and promotes the process of luteolysis, thereby stimulating the release of PGF2α. On the other hand, other investigators [28] suggest that TNF-α may provide both luteolytic and luteotropic tendencies. Thus, the increased concenrations of P4 in pregnant animals may have played a role in inhibiting the luteolytic properties of TNF-α [49, 50]; nevertheless, the decreasing concentrations of TNF-α in the nonpregnant cows, seems to be associated with the luteolytic properties and consequently low concentrations of P4 as it has been reported in some other species and in cattle [28, 51].


Dynamics of Progesterone, TNF- α , and a Metabolite of PGF2 α in Blood Plasma of Beef Cows following Embryo Transfer.

Mason MC, Copeland J, Cuadra EJ, Elsasser TH, Jung Y, Larson J - Vet Med Int (2014)

Concentrations (Mean ± SEM) of TNF-α in pregnant and nonpregnant cows on d 7, d 14, and d 21. Pregnancy status x day interaction (P ≤ 0.05); CIDR = controlled internal drug release; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4202664&req=5

fig4: Concentrations (Mean ± SEM) of TNF-α in pregnant and nonpregnant cows on d 7, d 14, and d 21. Pregnancy status x day interaction (P ≤ 0.05); CIDR = controlled internal drug release; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin.
Mentions: An overall comparison between pregnant and nonpregnant animals (Figure 4) showed a decrease (P ≤ 0.05) in concentration of TNF-α from d 7 to d 21 in nonpregnant cows, which occurred similarly in P4 in this same experimental group. Previously, it has been reported by our laboratory that low concentrations of  TNF-α  are linked to low concentrations of P4 in nonpregnant cows [10]. The nonpregnant group additionally showed a greater (P ≤ 0.05) concentration of TNF-α on d 7 compared to the pregnant group for reasons unable to be determined with these results. However, it is noteworthy that contrary to nonpregnant animals, pregnant cows maintained steadier concentrations of TNF-α through the entire experimental period. Interestingly, TNF-α has been reported having luteolytic properties. Some investigators [46–48] have suggested that TNF-α is deleterious to young embryos and promotes the process of luteolysis, thereby stimulating the release of PGF2α. On the other hand, other investigators [28] suggest that TNF-α may provide both luteolytic and luteotropic tendencies. Thus, the increased concenrations of P4 in pregnant animals may have played a role in inhibiting the luteolytic properties of TNF-α [49, 50]; nevertheless, the decreasing concentrations of TNF-α in the nonpregnant cows, seems to be associated with the luteolytic properties and consequently low concentrations of P4 as it has been reported in some other species and in cattle [28, 51].

Bottom Line: Blood was collected (every 15 min for 2 h) in half the animals in each treatment group on d 14 and the remaining half on d 21 for analysis of prostaglandin F2α metabolite (PGFM).Progesterone was greater (P ≤ 0.05) in cows receiving hCG compared to others on d 14.Progesterone in all treatment groups increased from d 7 to d 14 and declined (P ≤ 0.05) from d 14 to d 21.

View Article: PubMed Central - PubMed

Affiliation: Department of Agriculture, Alcorn State University, 1000 ASU Drive No. 750, Alcorn State, Lorman, MS 39096, USA.

ABSTRACT
Lactating beef cows previously synchronized for estrus (d 0) were assigned to four treatments to assess their effectiveness in increasing blood progesterone (P4) and its effects on tumor necrosis factor-α (TNF-α) and prostaglandin F2α (PGF2α) after the transfer of embryos. At the time of transfer (d 7), cows received no treatment (control; n = 16), a controlled internal drug releasing device (CIDR; n = 16), human chorionic gonadotropin (hCG; n = 15), or gonadotropin releasing hormone (GnRH; n = 15). Blood samples were taken on d 7, 14, and 21 for analysis of P4 and tumor necrosis factor-α (TNF-α). Blood was collected (every 15 min for 2 h) in half the animals in each treatment group on d 14 and the remaining half on d 21 for analysis of prostaglandin F2α metabolite (PGFM). Retention rates were 56.2, 62.5, 46.7, and 13.3% for cows in the control, CIDR, hCG, and GnRH groups, respectively. Progesterone was greater (P ≤ 0.05) in cows receiving hCG compared to others on d 14. Progesterone in all treatment groups increased from d 7 to d 14 and declined (P ≤ 0.05) from d 14 to d 21. Contrary to pregnant cows, P4 and TNF-α declined from d 7 to d 21 in nonpregnant cows (P ≤ 0.05). Although PGFM increased by d 21, there was no difference between pregnant and nonpregnant cows.

No MeSH data available.


Related in: MedlinePlus