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Fluoxetin upregulates connexin 43 expression in astrocyte.

Mostafavi H, Khaksarian M, Joghataei MT, Hassanzadeh G, Soleimani M, Eftekhari S, Soleimani M, Mousavizadeh K, Hadjighassem MR - Basic Clin Neurosci (2014)

Bottom Line: Fluoxetine had some effects in MS patients unrelated to its known antidepressant effects.In conclusion, data showed that fluoxetine alone and in the absence of serotonin acutely up-regulated Cx43 expression in astrocytes that can be assumed in molecular target therapy of MS patients.It seems that cAMP involvement in fluoxetine effects need more researches.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran ; Division of Neuroscience, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran ; Stem Cell Technology Research Center, Molecular Biology and Genetic Engineering Department, Tehran, Iran ; Department of Physiology and Pharmacology, Zanjan University of Medical Sciences, Zanjan, Iran.

ABSTRACT

Introduction: Recent studies have shown that astrocytes play major roles in normal and disease condition of the central nervous system including multiple sclerosis (MS). Molecular target therapy studies in MS have revealed that connexin-43 (Cx43) and Aquaporin-4 (AQP4) contents of astrocytes undergo expression alteration. Fluoxetine had some effects in MS patients unrelated to its known antidepressant effects. Some of fluoxetine effects were attributed to its capability of cAMP signaling pathway stimulation. This study aimed to investigate possible acute effects of fluoxetine on Cx43 and AQP4 expression in astrocyte.

Methods: Astrocytoma cells were treated for 24 hours with fluoxetine (10 and 20 µg/ml) with or without adenyl cyclase (AC) and protein kinase A (PKA) inhibition. Cx43 expression at both mRNA and protein levels and AQP4 expression at mRNA level were evaluated.

Results: Acquired results showed that fluoxetine with and without AC and PKA inhibition resulted in Cx43 up-regulation both in mRNA and protein levels, whereas AQP4 expression have not changed.

Discussion: In conclusion, data showed that fluoxetine alone and in the absence of serotonin acutely up-regulated Cx43 expression in astrocytes that can be assumed in molecular target therapy of MS patients. It seems that cAMP involvement in fluoxetine effects need more researches.

No MeSH data available.


Related in: MedlinePlus

Relative expression of Epac in 1321N1 (Fig. 1A) and U87MG (Fig. 1B) cell lines in un-treated (C) and treated cells with 10 µg/ml fluoxetine (F) HPRT1 was used as internal control.* P < 0.05; ** P < 0.01; PTZ, Pentylenetetrazol; Ctrl, Control; SB, SB334867; ORX, Orexin-A; Lido., lidocaine
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Figure 0001: Relative expression of Epac in 1321N1 (Fig. 1A) and U87MG (Fig. 1B) cell lines in un-treated (C) and treated cells with 10 µg/ml fluoxetine (F) HPRT1 was used as internal control.* P < 0.05; ** P < 0.01; PTZ, Pentylenetetrazol; Ctrl, Control; SB, SB334867; ORX, Orexin-A; Lido., lidocaine

Mentions: To evaluate possible effect of fluoxetine on expression of EPAC in U87MG and 1321N1 cell lines, we performed Real-time PCR analysis on cDNA obtained from these cells using specific primers for detection of EPAC. We found that fluoxetine did not change the expression of EPAC mRNA in both cell lines (Fig. 1).


Fluoxetin upregulates connexin 43 expression in astrocyte.

Mostafavi H, Khaksarian M, Joghataei MT, Hassanzadeh G, Soleimani M, Eftekhari S, Soleimani M, Mousavizadeh K, Hadjighassem MR - Basic Clin Neurosci (2014)

Relative expression of Epac in 1321N1 (Fig. 1A) and U87MG (Fig. 1B) cell lines in un-treated (C) and treated cells with 10 µg/ml fluoxetine (F) HPRT1 was used as internal control.* P < 0.05; ** P < 0.01; PTZ, Pentylenetetrazol; Ctrl, Control; SB, SB334867; ORX, Orexin-A; Lido., lidocaine
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4202606&req=5

Figure 0001: Relative expression of Epac in 1321N1 (Fig. 1A) and U87MG (Fig. 1B) cell lines in un-treated (C) and treated cells with 10 µg/ml fluoxetine (F) HPRT1 was used as internal control.* P < 0.05; ** P < 0.01; PTZ, Pentylenetetrazol; Ctrl, Control; SB, SB334867; ORX, Orexin-A; Lido., lidocaine
Mentions: To evaluate possible effect of fluoxetine on expression of EPAC in U87MG and 1321N1 cell lines, we performed Real-time PCR analysis on cDNA obtained from these cells using specific primers for detection of EPAC. We found that fluoxetine did not change the expression of EPAC mRNA in both cell lines (Fig. 1).

Bottom Line: Fluoxetine had some effects in MS patients unrelated to its known antidepressant effects.In conclusion, data showed that fluoxetine alone and in the absence of serotonin acutely up-regulated Cx43 expression in astrocytes that can be assumed in molecular target therapy of MS patients.It seems that cAMP involvement in fluoxetine effects need more researches.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran ; Division of Neuroscience, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran ; Stem Cell Technology Research Center, Molecular Biology and Genetic Engineering Department, Tehran, Iran ; Department of Physiology and Pharmacology, Zanjan University of Medical Sciences, Zanjan, Iran.

ABSTRACT

Introduction: Recent studies have shown that astrocytes play major roles in normal and disease condition of the central nervous system including multiple sclerosis (MS). Molecular target therapy studies in MS have revealed that connexin-43 (Cx43) and Aquaporin-4 (AQP4) contents of astrocytes undergo expression alteration. Fluoxetine had some effects in MS patients unrelated to its known antidepressant effects. Some of fluoxetine effects were attributed to its capability of cAMP signaling pathway stimulation. This study aimed to investigate possible acute effects of fluoxetine on Cx43 and AQP4 expression in astrocyte.

Methods: Astrocytoma cells were treated for 24 hours with fluoxetine (10 and 20 µg/ml) with or without adenyl cyclase (AC) and protein kinase A (PKA) inhibition. Cx43 expression at both mRNA and protein levels and AQP4 expression at mRNA level were evaluated.

Results: Acquired results showed that fluoxetine with and without AC and PKA inhibition resulted in Cx43 up-regulation both in mRNA and protein levels, whereas AQP4 expression have not changed.

Discussion: In conclusion, data showed that fluoxetine alone and in the absence of serotonin acutely up-regulated Cx43 expression in astrocytes that can be assumed in molecular target therapy of MS patients. It seems that cAMP involvement in fluoxetine effects need more researches.

No MeSH data available.


Related in: MedlinePlus