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Effect of Chronic Restraint Stress on HPA Axis Activity and Expression of BDNF and Trkb in the Hippocampus of Pregnant Rats: Possible Contribution in Depression during Pregnancy and Postpartum Period.

Maghsoudi N, Ghasemi R, Ghaempanah Z, Ardekani AM, Nooshinfar E, Tahzibi A - Basic Clin Neurosci (2014)

Bottom Line: Brain-Derived Neurotrophic Factor (BDNF) and its receptor, TrkB, in the hippocampus are targets for adverse effects of stress paradigms; in addition, BDNF and its receptor play key role in the pathology of brain diseases like depression.The results showed that in stress group the level of ACTH and Corticosterone is increased showing that our model was efficient in inducing psychological stress; we also found that BDNF and TrkB expression are decreased in 3 hours stress group but not in 1 hour stress compared to control group.Our results imply that decrease in BDNF and its receptor could contribute in some adverse effects of stress during pregnancy such as elevation of depressive like behavior.

View Article: PubMed Central - PubMed

Affiliation: Neurobiology Research Center and Neuroscience Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Brain-Derived Neurotrophic Factor (BDNF) and its receptor, TrkB, in the hippocampus are targets for adverse effects of stress paradigms; in addition, BDNF and its receptor play key role in the pathology of brain diseases like depression. In the present study, we evaluated the possible role of hippocampal BDNF in depression during pregnancy.

Methods: To achieve the purpose, repeated restrain stress (1 or 3 hours daily for 7 days) during the last week of pregnancy was used and alteration in the gene expression of hippocampal BDNF and TrkB evaluated by semi-quantitative PCR.

Results: The results showed that in stress group the level of ACTH and Corticosterone is increased showing that our model was efficient in inducing psychological stress; we also found that BDNF and TrkB expression are decreased in 3 hours stress group but not in 1 hour stress compared to control group.

Discussion: Our results imply that decrease in BDNF and its receptor could contribute in some adverse effects of stress during pregnancy such as elevation of depressive like behavior.

No MeSH data available.


Related in: MedlinePlus

Stress induced changes in expression of the TrkB gene. (A) The graph shows the mean ratio of TrkB/GAPDH of three groups. (B) The band density of TrkB and GAPDH for each group can be seen. Data are represented as mean ± SEM. **p < 0.01 represent the difference between control and stress groups.
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Figure 0003: Stress induced changes in expression of the TrkB gene. (A) The graph shows the mean ratio of TrkB/GAPDH of three groups. (B) The band density of TrkB and GAPDH for each group can be seen. Data are represented as mean ± SEM. **p < 0.01 represent the difference between control and stress groups.

Mentions: Comparison of the normalized ratio of TrkB/GAPDH for expression of TrkB revealed a similar pattern of expression for TrkB. In the 1 hour group, TrkB expression was reduced but it was not quite significant (P= 0.068) in the 3 hours group in which TrkB was reduced significantly (P= 0.004). These results can be seen in Figures 3A and 3B.


Effect of Chronic Restraint Stress on HPA Axis Activity and Expression of BDNF and Trkb in the Hippocampus of Pregnant Rats: Possible Contribution in Depression during Pregnancy and Postpartum Period.

Maghsoudi N, Ghasemi R, Ghaempanah Z, Ardekani AM, Nooshinfar E, Tahzibi A - Basic Clin Neurosci (2014)

Stress induced changes in expression of the TrkB gene. (A) The graph shows the mean ratio of TrkB/GAPDH of three groups. (B) The band density of TrkB and GAPDH for each group can be seen. Data are represented as mean ± SEM. **p < 0.01 represent the difference between control and stress groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4202592&req=5

Figure 0003: Stress induced changes in expression of the TrkB gene. (A) The graph shows the mean ratio of TrkB/GAPDH of three groups. (B) The band density of TrkB and GAPDH for each group can be seen. Data are represented as mean ± SEM. **p < 0.01 represent the difference between control and stress groups.
Mentions: Comparison of the normalized ratio of TrkB/GAPDH for expression of TrkB revealed a similar pattern of expression for TrkB. In the 1 hour group, TrkB expression was reduced but it was not quite significant (P= 0.068) in the 3 hours group in which TrkB was reduced significantly (P= 0.004). These results can be seen in Figures 3A and 3B.

Bottom Line: Brain-Derived Neurotrophic Factor (BDNF) and its receptor, TrkB, in the hippocampus are targets for adverse effects of stress paradigms; in addition, BDNF and its receptor play key role in the pathology of brain diseases like depression.The results showed that in stress group the level of ACTH and Corticosterone is increased showing that our model was efficient in inducing psychological stress; we also found that BDNF and TrkB expression are decreased in 3 hours stress group but not in 1 hour stress compared to control group.Our results imply that decrease in BDNF and its receptor could contribute in some adverse effects of stress during pregnancy such as elevation of depressive like behavior.

View Article: PubMed Central - PubMed

Affiliation: Neurobiology Research Center and Neuroscience Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Brain-Derived Neurotrophic Factor (BDNF) and its receptor, TrkB, in the hippocampus are targets for adverse effects of stress paradigms; in addition, BDNF and its receptor play key role in the pathology of brain diseases like depression. In the present study, we evaluated the possible role of hippocampal BDNF in depression during pregnancy.

Methods: To achieve the purpose, repeated restrain stress (1 or 3 hours daily for 7 days) during the last week of pregnancy was used and alteration in the gene expression of hippocampal BDNF and TrkB evaluated by semi-quantitative PCR.

Results: The results showed that in stress group the level of ACTH and Corticosterone is increased showing that our model was efficient in inducing psychological stress; we also found that BDNF and TrkB expression are decreased in 3 hours stress group but not in 1 hour stress compared to control group.

Discussion: Our results imply that decrease in BDNF and its receptor could contribute in some adverse effects of stress during pregnancy such as elevation of depressive like behavior.

No MeSH data available.


Related in: MedlinePlus