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Effect of Chronic Restraint Stress on HPA Axis Activity and Expression of BDNF and Trkb in the Hippocampus of Pregnant Rats: Possible Contribution in Depression during Pregnancy and Postpartum Period.

Maghsoudi N, Ghasemi R, Ghaempanah Z, Ardekani AM, Nooshinfar E, Tahzibi A - Basic Clin Neurosci (2014)

Bottom Line: Brain-Derived Neurotrophic Factor (BDNF) and its receptor, TrkB, in the hippocampus are targets for adverse effects of stress paradigms; in addition, BDNF and its receptor play key role in the pathology of brain diseases like depression.The results showed that in stress group the level of ACTH and Corticosterone is increased showing that our model was efficient in inducing psychological stress; we also found that BDNF and TrkB expression are decreased in 3 hours stress group but not in 1 hour stress compared to control group.Our results imply that decrease in BDNF and its receptor could contribute in some adverse effects of stress during pregnancy such as elevation of depressive like behavior.

View Article: PubMed Central - PubMed

Affiliation: Neurobiology Research Center and Neuroscience Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Brain-Derived Neurotrophic Factor (BDNF) and its receptor, TrkB, in the hippocampus are targets for adverse effects of stress paradigms; in addition, BDNF and its receptor play key role in the pathology of brain diseases like depression. In the present study, we evaluated the possible role of hippocampal BDNF in depression during pregnancy.

Methods: To achieve the purpose, repeated restrain stress (1 or 3 hours daily for 7 days) during the last week of pregnancy was used and alteration in the gene expression of hippocampal BDNF and TrkB evaluated by semi-quantitative PCR.

Results: The results showed that in stress group the level of ACTH and Corticosterone is increased showing that our model was efficient in inducing psychological stress; we also found that BDNF and TrkB expression are decreased in 3 hours stress group but not in 1 hour stress compared to control group.

Discussion: Our results imply that decrease in BDNF and its receptor could contribute in some adverse effects of stress during pregnancy such as elevation of depressive like behavior.

No MeSH data available.


Related in: MedlinePlus

The effect of restraint stress on plasma ACTH and Corticosterone levels. (A) Plasma ACTH levels after 1 and 3 h repeated stress episodes. (B) Plasma Corticosterone levels after 1 and 3 h repeated stress episode. Data are represented as mean ± SEM. ***p < 0.001, **p < 0.01 and *p < 0.05 represent the difference between control and stress groups.
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Figure 0001: The effect of restraint stress on plasma ACTH and Corticosterone levels. (A) Plasma ACTH levels after 1 and 3 h repeated stress episodes. (B) Plasma Corticosterone levels after 1 and 3 h repeated stress episode. Data are represented as mean ± SEM. ***p < 0.001, **p < 0.01 and *p < 0.05 represent the difference between control and stress groups.

Mentions: To test the efficiency of our stress paradigm, Hypothalamic-Pituitary-Adrenal (HPA) axis activity assessment was done and showed significant increase in ACTH both in 1 hour stress and 3 hours stress (p <0.05 for 1 hour group and <0.01 for 3 hours group, Figure 1A). As expected from ACTH results, corticosterone level was also elevated in the both stress group (p <0.001, Figure 1B).


Effect of Chronic Restraint Stress on HPA Axis Activity and Expression of BDNF and Trkb in the Hippocampus of Pregnant Rats: Possible Contribution in Depression during Pregnancy and Postpartum Period.

Maghsoudi N, Ghasemi R, Ghaempanah Z, Ardekani AM, Nooshinfar E, Tahzibi A - Basic Clin Neurosci (2014)

The effect of restraint stress on plasma ACTH and Corticosterone levels. (A) Plasma ACTH levels after 1 and 3 h repeated stress episodes. (B) Plasma Corticosterone levels after 1 and 3 h repeated stress episode. Data are represented as mean ± SEM. ***p < 0.001, **p < 0.01 and *p < 0.05 represent the difference between control and stress groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4202592&req=5

Figure 0001: The effect of restraint stress on plasma ACTH and Corticosterone levels. (A) Plasma ACTH levels after 1 and 3 h repeated stress episodes. (B) Plasma Corticosterone levels after 1 and 3 h repeated stress episode. Data are represented as mean ± SEM. ***p < 0.001, **p < 0.01 and *p < 0.05 represent the difference between control and stress groups.
Mentions: To test the efficiency of our stress paradigm, Hypothalamic-Pituitary-Adrenal (HPA) axis activity assessment was done and showed significant increase in ACTH both in 1 hour stress and 3 hours stress (p <0.05 for 1 hour group and <0.01 for 3 hours group, Figure 1A). As expected from ACTH results, corticosterone level was also elevated in the both stress group (p <0.001, Figure 1B).

Bottom Line: Brain-Derived Neurotrophic Factor (BDNF) and its receptor, TrkB, in the hippocampus are targets for adverse effects of stress paradigms; in addition, BDNF and its receptor play key role in the pathology of brain diseases like depression.The results showed that in stress group the level of ACTH and Corticosterone is increased showing that our model was efficient in inducing psychological stress; we also found that BDNF and TrkB expression are decreased in 3 hours stress group but not in 1 hour stress compared to control group.Our results imply that decrease in BDNF and its receptor could contribute in some adverse effects of stress during pregnancy such as elevation of depressive like behavior.

View Article: PubMed Central - PubMed

Affiliation: Neurobiology Research Center and Neuroscience Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Brain-Derived Neurotrophic Factor (BDNF) and its receptor, TrkB, in the hippocampus are targets for adverse effects of stress paradigms; in addition, BDNF and its receptor play key role in the pathology of brain diseases like depression. In the present study, we evaluated the possible role of hippocampal BDNF in depression during pregnancy.

Methods: To achieve the purpose, repeated restrain stress (1 or 3 hours daily for 7 days) during the last week of pregnancy was used and alteration in the gene expression of hippocampal BDNF and TrkB evaluated by semi-quantitative PCR.

Results: The results showed that in stress group the level of ACTH and Corticosterone is increased showing that our model was efficient in inducing psychological stress; we also found that BDNF and TrkB expression are decreased in 3 hours stress group but not in 1 hour stress compared to control group.

Discussion: Our results imply that decrease in BDNF and its receptor could contribute in some adverse effects of stress during pregnancy such as elevation of depressive like behavior.

No MeSH data available.


Related in: MedlinePlus