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Interaction between Antagonist of Cannabinoid Receptor and Antagonist of Adrenergic Receptor on Anxiety in Male Rat.

Komaki A, Abdollahzadeh F, Sarihi A, Shahidi S, Salehi I - Basic Clin Neurosci (2014)

Bottom Line: Diazepam was considered as a positive control drug with anxiolytic effect (0.3, 0.6, 1.2 mg/kg).Diazepam increased the number of open arm entries and the percentage of spent time on the open arms.Further investigations are essential for better understanding of anxiolytic and anxiogenic properties and neurobiological mechanisms of action and probable interactions of the two systems.

View Article: PubMed Central - PubMed

Affiliation: Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

ABSTRACT

Introduction: Anxiety is among the most common and treatable mental disorders. Adrenergic and cannabinoid systems have an important role in the neurobiology of anxiety. The elevated plus-maze (EPM) has broadly been used to investigate anxiolytic and anxiogenic compounds. The present study investigated the effects of intraperitoneal (IP) injection of cannabinoid CB1 receptor antagonist (AM251) in the presence of alpha-1 adrenergic antagonist (Prazosin) on rat behavior in the EPM.

Methods: In this study, the data were obtained from male Wistar rat, which weighing 200- 250 g. Animal behavior in EPM were videotaped and saved in computer for 10 min after IP injection of saline, AM251 (0.3 mg/kg), Prazosin (0.3 mg/kg) and AM251 + Prazosin, subsequently scored for conventional indices of anxiety. During the test period, the number of open and closed arms entries, the percentage of entries into the open arms of the EPM, and the spent time in open and closed arms were recorded. Diazepam was considered as a positive control drug with anxiolytic effect (0.3, 0.6, 1.2 mg/kg).

Results: Diazepam increased the number of open arm entries and the percentage of spent time on the open arms. IP injection of AM251 before EPM trial decreased open arms exploration and open arm entry. Whereas, Prazosin increased open arms exploration and open arm entry. This study showed that both substances in simultaneous injection have conflicting effects on the responses of each of these two compounds in a single injection.

Discussion: Injection of CB1 receptor antagonist may have an anxiogenic profile in rat, whereas adrenergic antagonist has an anxiolytic effect. Further investigations are essential for better understanding of anxiolytic and anxiogenic properties and neurobiological mechanisms of action and probable interactions of the two systems.

No MeSH data available.


Related in: MedlinePlus

The effects of diazepam (0.3, 0.6, 1.2 mg/kg i.p.) on the percentage of entries in open arms (A), spent time in open arms (B) and number of closed arms entry (C) during the 10-min test session in EPM. Data represent means±SEM.*: P < 0.05, **: P < 0.01 in comparison with control group.
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Figure 0001: The effects of diazepam (0.3, 0.6, 1.2 mg/kg i.p.) on the percentage of entries in open arms (A), spent time in open arms (B) and number of closed arms entry (C) during the 10-min test session in EPM. Data represent means±SEM.*: P < 0.05, **: P < 0.01 in comparison with control group.

Mentions: In this study, at first the effect of diazepam on rat behavior was surveyed to estimate the plus-maze set usage correction. Diazepam was considered as a positive control drug with anxiolytic effect)Souto-Maior et al., 2011; Gomes et al., 2010). This anxiolytic drug with low dose (0.3 mg/kg) showed significant increase in the percent of open arms entry (OE) (Figure 1-A), and the spent time on open arms (OT) (Figure 1-B) [F (3, 36) = 5.81, p < 0.01]. Diazepam showed significant increase in the open arms exploration in concentrations of 0.3 [F (3, 36) = 5.06, p <0.01] and 0.6 mg/kg [F (3, 36) = 3.33, p < 0.05], but interestingly not at 1.2 mg/kg (Figure 1-A, B). The number of closed arms entries was significantly [F (3, 36) = 2.89, p <0.05] different for the group that received 1.2 mg/kg of diazepam. But, the number of closed arms entries was not significantly different for the groups that received 0.3 or 0.6 mg/kg of diazepam (Fig. 1C).


Interaction between Antagonist of Cannabinoid Receptor and Antagonist of Adrenergic Receptor on Anxiety in Male Rat.

Komaki A, Abdollahzadeh F, Sarihi A, Shahidi S, Salehi I - Basic Clin Neurosci (2014)

The effects of diazepam (0.3, 0.6, 1.2 mg/kg i.p.) on the percentage of entries in open arms (A), spent time in open arms (B) and number of closed arms entry (C) during the 10-min test session in EPM. Data represent means±SEM.*: P < 0.05, **: P < 0.01 in comparison with control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4202541&req=5

Figure 0001: The effects of diazepam (0.3, 0.6, 1.2 mg/kg i.p.) on the percentage of entries in open arms (A), spent time in open arms (B) and number of closed arms entry (C) during the 10-min test session in EPM. Data represent means±SEM.*: P < 0.05, **: P < 0.01 in comparison with control group.
Mentions: In this study, at first the effect of diazepam on rat behavior was surveyed to estimate the plus-maze set usage correction. Diazepam was considered as a positive control drug with anxiolytic effect)Souto-Maior et al., 2011; Gomes et al., 2010). This anxiolytic drug with low dose (0.3 mg/kg) showed significant increase in the percent of open arms entry (OE) (Figure 1-A), and the spent time on open arms (OT) (Figure 1-B) [F (3, 36) = 5.81, p < 0.01]. Diazepam showed significant increase in the open arms exploration in concentrations of 0.3 [F (3, 36) = 5.06, p <0.01] and 0.6 mg/kg [F (3, 36) = 3.33, p < 0.05], but interestingly not at 1.2 mg/kg (Figure 1-A, B). The number of closed arms entries was significantly [F (3, 36) = 2.89, p <0.05] different for the group that received 1.2 mg/kg of diazepam. But, the number of closed arms entries was not significantly different for the groups that received 0.3 or 0.6 mg/kg of diazepam (Fig. 1C).

Bottom Line: Diazepam was considered as a positive control drug with anxiolytic effect (0.3, 0.6, 1.2 mg/kg).Diazepam increased the number of open arm entries and the percentage of spent time on the open arms.Further investigations are essential for better understanding of anxiolytic and anxiogenic properties and neurobiological mechanisms of action and probable interactions of the two systems.

View Article: PubMed Central - PubMed

Affiliation: Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

ABSTRACT

Introduction: Anxiety is among the most common and treatable mental disorders. Adrenergic and cannabinoid systems have an important role in the neurobiology of anxiety. The elevated plus-maze (EPM) has broadly been used to investigate anxiolytic and anxiogenic compounds. The present study investigated the effects of intraperitoneal (IP) injection of cannabinoid CB1 receptor antagonist (AM251) in the presence of alpha-1 adrenergic antagonist (Prazosin) on rat behavior in the EPM.

Methods: In this study, the data were obtained from male Wistar rat, which weighing 200- 250 g. Animal behavior in EPM were videotaped and saved in computer for 10 min after IP injection of saline, AM251 (0.3 mg/kg), Prazosin (0.3 mg/kg) and AM251 + Prazosin, subsequently scored for conventional indices of anxiety. During the test period, the number of open and closed arms entries, the percentage of entries into the open arms of the EPM, and the spent time in open and closed arms were recorded. Diazepam was considered as a positive control drug with anxiolytic effect (0.3, 0.6, 1.2 mg/kg).

Results: Diazepam increased the number of open arm entries and the percentage of spent time on the open arms. IP injection of AM251 before EPM trial decreased open arms exploration and open arm entry. Whereas, Prazosin increased open arms exploration and open arm entry. This study showed that both substances in simultaneous injection have conflicting effects on the responses of each of these two compounds in a single injection.

Discussion: Injection of CB1 receptor antagonist may have an anxiogenic profile in rat, whereas adrenergic antagonist has an anxiolytic effect. Further investigations are essential for better understanding of anxiolytic and anxiogenic properties and neurobiological mechanisms of action and probable interactions of the two systems.

No MeSH data available.


Related in: MedlinePlus