Characterization of newly gained introns in Daphnia populations.
Bottom Line: A disproportionally large number of new introns were found in historically isolated populations in Oregon.A majority (55/90 or 61.1%) of the identified neointrons have associated internal direct repeats with lengths and compositions that are unlikely to occur by chance, suggesting repeated bouts of staggered double-strand breaks (DSBs) during their evolution.Accordingly, internal, staggered DSBs may contribute to a passive trend toward increased length and sequence diversity in nascent introns.
Affiliation: Department of Pediatrics, Section of Genomic Pediatrics, Medical College of Wisconsin firstname.lastname@example.org.Show MeSH
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Mentions: Based on sequence characteristics of these newly gained introns and adjacent exons, observed events of intron gain are associated with initial DSBs. Some appear to have been formed through staggered DSBs, evidenced by direct repeats at opposite ends. Others show evidence of a combination of blunt and staggered DSBs at the same site, although the order of these two events is sometimes unclear (fig. 3 and supplementary figs. S1, S21, S40, and S58c, Supplementary Material online).Fig. 3.—
Affiliation: Department of Pediatrics, Section of Genomic Pediatrics, Medical College of Wisconsin email@example.com.