Ionizing radiation affects the expression of Toll-like receptors 2 and 4 in human monocytic cells through c-Jun N-terminal kinase activation.
Bottom Line: Pattern recognition receptors recognize pathogen-associated molecular patterns.X-irradiation caused increased expression of these TLRs in THP1 and decreased expression in macrophage-like cells.However, although TNF-α expression was not affected by X-irradiation in macrophage-like cells, the expression of LPS-inducible interferon-β was lower following X-irradiation of macrophage-like cells.
Affiliation: Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan.Show MeSH
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Mentions: THP1-derived macrophages (macrophage-like cells) were prepared as previously described . Briefly, THP1 monocytes (2.0 × 105 cells/ml) were plated in 60 mm dishes (IWAKI, Tokyo, Japan) with 4 ml of medium containing 100 ng/ml PMA, and were cultured for 48 h. Differentiation to macrophage-like cells was confirmed by observing morphological changes under a microscope, as shown in Fig. 1A. After 48-h culture, the medium containing PMA was replaced with fresh medium not containing PMA, and macrophage-like cells were then used in experiments. Adherent macrophage-like cells were harvested by trypsinization with 0.1% trypsin/ethylenediaminetetraacetate (Gibco). Viable cell numbers were estimated using trypan blue dye exclusion assays.Fig. 1.
Affiliation: Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan.