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Applicability of the methylated CpG sites of paired box 5 (PAX5) promoter for prediction the prognosis of gastric cancer.

Deng J, Liang H, Zhang R, Dong Q, Hou Y, Yu J, Fan D, Hao X - Oncotarget (2014)

Bottom Line: At present study, we found that the inconsistently methylated levels of PAX5 promoter were identified in the different GC tissues.The methylated CpG site count and the methylated statuses of four CpG sites (-236, -183, -162, and -152) were significantly associated with the survival of 460 GC patients, respectively.These findings indicated that the methylated CpG -236 of PAX5 promoter has the potential applicability for clinical evaluation the prognosis of GC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer, Tianjin, China.

ABSTRACT
Paired box gene 5 (PAX5), a member of the paired box gene family, is involved in control of organ development and tissue differentiation. In previous study, PAX5 promoter methylation was found in gastric cancer (GC) cells and tissues. At present study, we found that the inconsistently methylated levels of PAX5 promoter were identified in the different GC tissues. The methylated CpG site count and the methylated statuses of four CpG sites (-236, -183, -162, and -152) were significantly associated with the survival of 460 GC patients, respectively. Ultimately, the methylated CpG -236 was the optimal prognostic predictor of patients identified by using the Cox regression with AIC value calculation. These findings indicated that the methylated CpG -236 of PAX5 promoter has the potential applicability for clinical evaluation the prognosis of GC.

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Related in: MedlinePlus

(A) PAX5 mRNA expression (RT-PCR) in GC tissues and in normal gastric mucosal tissues; (B) Western Blot analysis for PAX5 protein expression in GC tissues and in normal gastric mucosal tissues. (Representation: T, GC tissues; N, normal gastric mucosal tissues).
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Figure 1: (A) PAX5 mRNA expression (RT-PCR) in GC tissues and in normal gastric mucosal tissues; (B) Western Blot analysis for PAX5 protein expression in GC tissues and in normal gastric mucosal tissues. (Representation: T, GC tissues; N, normal gastric mucosal tissues).

Mentions: PAX5 mRNA expression was detected in 25 of 460 GC tissues and 25 normal gastric mucosal tissues by Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) (Figure 1A). We also found there were significant differences of PAX5 mRNA expression in 25 GC tissues. The mean value of relative mRNA expression of PAX5 in 25 GC tissues was 0.863±0.357, while the mean value of relative mRNA expression of PAX5 in 25 normal gastric mucosal tissues was 1.759±0.821. The mean value of relative mRNA expression of PAX5 in 25GC tissues was lower than that in 25 normal gastric mucosal tissues (P =0.036).


Applicability of the methylated CpG sites of paired box 5 (PAX5) promoter for prediction the prognosis of gastric cancer.

Deng J, Liang H, Zhang R, Dong Q, Hou Y, Yu J, Fan D, Hao X - Oncotarget (2014)

(A) PAX5 mRNA expression (RT-PCR) in GC tissues and in normal gastric mucosal tissues; (B) Western Blot analysis for PAX5 protein expression in GC tissues and in normal gastric mucosal tissues. (Representation: T, GC tissues; N, normal gastric mucosal tissues).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4202133&req=5

Figure 1: (A) PAX5 mRNA expression (RT-PCR) in GC tissues and in normal gastric mucosal tissues; (B) Western Blot analysis for PAX5 protein expression in GC tissues and in normal gastric mucosal tissues. (Representation: T, GC tissues; N, normal gastric mucosal tissues).
Mentions: PAX5 mRNA expression was detected in 25 of 460 GC tissues and 25 normal gastric mucosal tissues by Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) (Figure 1A). We also found there were significant differences of PAX5 mRNA expression in 25 GC tissues. The mean value of relative mRNA expression of PAX5 in 25 GC tissues was 0.863±0.357, while the mean value of relative mRNA expression of PAX5 in 25 normal gastric mucosal tissues was 1.759±0.821. The mean value of relative mRNA expression of PAX5 in 25GC tissues was lower than that in 25 normal gastric mucosal tissues (P =0.036).

Bottom Line: At present study, we found that the inconsistently methylated levels of PAX5 promoter were identified in the different GC tissues.The methylated CpG site count and the methylated statuses of four CpG sites (-236, -183, -162, and -152) were significantly associated with the survival of 460 GC patients, respectively.These findings indicated that the methylated CpG -236 of PAX5 promoter has the potential applicability for clinical evaluation the prognosis of GC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer, Tianjin, China.

ABSTRACT
Paired box gene 5 (PAX5), a member of the paired box gene family, is involved in control of organ development and tissue differentiation. In previous study, PAX5 promoter methylation was found in gastric cancer (GC) cells and tissues. At present study, we found that the inconsistently methylated levels of PAX5 promoter were identified in the different GC tissues. The methylated CpG site count and the methylated statuses of four CpG sites (-236, -183, -162, and -152) were significantly associated with the survival of 460 GC patients, respectively. Ultimately, the methylated CpG -236 was the optimal prognostic predictor of patients identified by using the Cox regression with AIC value calculation. These findings indicated that the methylated CpG -236 of PAX5 promoter has the potential applicability for clinical evaluation the prognosis of GC.

Show MeSH
Related in: MedlinePlus