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Therapeutic effects of amniotic fluid-derived mesenchymal stromal cells on lung injury in rats with emphysema.

Li Y, Gu C, Xu W, Yan J, Xia Y, Ma Y, Chen C, He X, Tao H - Respir. Res. (2014)

Bottom Line: Moreover, we examined the lung morphology and the expression levels of SPA and SPC in rats with emphysema after cigarette-smoke exposure and intratracheal lipopolysaccharide instillation and rAFMSC transplantation.In rAFMSCs, the surface antigens CD29, CD44, CD73, CD90, CD105, and CD166 were expressed, but CD14, CD19, CD34, and CD45 were not detected; rAFMSCs also strongly expressed the mRNA of octamer-binding transcription factor 4, and the cells could be induced to differentiate into adipocytes and osteocytes.Furthermore, rAFMSC treatment up-regulated the levels of SPA, SPC, and thyroid transcription factor 1 and inhibited AECII apoptosis, and rAFMSCs appeared to be capable of differentiating into AECII-like cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Zhejiang Provincial People's Hospital, No, 158, Shangtang Road, Hangzhou 310014, Zhejiang, P,R, China. lidoctor03@126.com.

ABSTRACT

Background: In chronic obstructive pulmonary disease (COPD), two major pathological changes that occur are the loss of alveolar structure and airspace enlargement. To treat COPD, it is crucial to repair damaged lung tissue and regenerate the lost alveoli. Type II alveolar epithelial cells (AECII) play a vital role in maintaining lung tissue repair, and amniotic fluid-derived mesenchymal stromal cells (AFMSCs) possess the characteristics of regular mesenchymal stromal cells. However, it remains untested whether transplantation of rat AFMSCs (rAFMSCs) might alleviate lung injury caused by emphysema by increasing the expression of surfactant protein (SP)A and SPC and inhibiting AECII apoptosis.

Methods: We analyzed the phenotypic characteristics, differentiation potential, and karyotype of rAFMSCs, which were isolated from pregnant Sprague-Dawley rats. Moreover, we examined the lung morphology and the expression levels of SPA and SPC in rats with emphysema after cigarette-smoke exposure and intratracheal lipopolysaccharide instillation and rAFMSC transplantation. The ability of rAFMSCs to differentiate was measured, and the apoptosis of AECII was evaluated.

Results: In rAFMSCs, the surface antigens CD29, CD44, CD73, CD90, CD105, and CD166 were expressed, but CD14, CD19, CD34, and CD45 were not detected; rAFMSCs also strongly expressed the mRNA of octamer-binding transcription factor 4, and the cells could be induced to differentiate into adipocytes and osteocytes. Furthermore, rAFMSC treatment up-regulated the levels of SPA, SPC, and thyroid transcription factor 1 and inhibited AECII apoptosis, and rAFMSCs appeared to be capable of differentiating into AECII-like cells. Lung injury caused by emphysema was alleviated after rAFMSC treatment.

Conclusions: rAFMSCs might differentiate into AECII-like cells or induce local regeneration of the lung alveolar epithelium in vivo after transplantation and thus could be used in COPD treatment and lung regenerative therapy.

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Differentiation potential of rAFMSCs. (A) Adipogenic differentiation, cells positive for Oil Red O staining. (B) Osteogenic differentiation, cells positive for alizarin red staining. Scale bars, 50 μm (A) and 200 μm (B).
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Fig3: Differentiation potential of rAFMSCs. (A) Adipogenic differentiation, cells positive for Oil Red O staining. (B) Osteogenic differentiation, cells positive for alizarin red staining. Scale bars, 50 μm (A) and 200 μm (B).

Mentions: To evaluate the differentiation potential of rAFMSCs, cells at P3 were induced to differentiate into adipocytes and osteocytes. After rAFMSCs were cultured in an adipogenic medium for 3 weeks, Oil Red O-positive intracellular lipid droplets were observed (Figure 3A). Similarly, after rAFMSCs were cultured in an osteogenic medium for 3 weeks, most of the differentiated cells appeared cubical and exhibited a dull-red alizarin-red staining that indicated calcium mineralization (Figure 3B).Figure 3


Therapeutic effects of amniotic fluid-derived mesenchymal stromal cells on lung injury in rats with emphysema.

Li Y, Gu C, Xu W, Yan J, Xia Y, Ma Y, Chen C, He X, Tao H - Respir. Res. (2014)

Differentiation potential of rAFMSCs. (A) Adipogenic differentiation, cells positive for Oil Red O staining. (B) Osteogenic differentiation, cells positive for alizarin red staining. Scale bars, 50 μm (A) and 200 μm (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4201761&req=5

Fig3: Differentiation potential of rAFMSCs. (A) Adipogenic differentiation, cells positive for Oil Red O staining. (B) Osteogenic differentiation, cells positive for alizarin red staining. Scale bars, 50 μm (A) and 200 μm (B).
Mentions: To evaluate the differentiation potential of rAFMSCs, cells at P3 were induced to differentiate into adipocytes and osteocytes. After rAFMSCs were cultured in an adipogenic medium for 3 weeks, Oil Red O-positive intracellular lipid droplets were observed (Figure 3A). Similarly, after rAFMSCs were cultured in an osteogenic medium for 3 weeks, most of the differentiated cells appeared cubical and exhibited a dull-red alizarin-red staining that indicated calcium mineralization (Figure 3B).Figure 3

Bottom Line: Moreover, we examined the lung morphology and the expression levels of SPA and SPC in rats with emphysema after cigarette-smoke exposure and intratracheal lipopolysaccharide instillation and rAFMSC transplantation.In rAFMSCs, the surface antigens CD29, CD44, CD73, CD90, CD105, and CD166 were expressed, but CD14, CD19, CD34, and CD45 were not detected; rAFMSCs also strongly expressed the mRNA of octamer-binding transcription factor 4, and the cells could be induced to differentiate into adipocytes and osteocytes.Furthermore, rAFMSC treatment up-regulated the levels of SPA, SPC, and thyroid transcription factor 1 and inhibited AECII apoptosis, and rAFMSCs appeared to be capable of differentiating into AECII-like cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Zhejiang Provincial People's Hospital, No, 158, Shangtang Road, Hangzhou 310014, Zhejiang, P,R, China. lidoctor03@126.com.

ABSTRACT

Background: In chronic obstructive pulmonary disease (COPD), two major pathological changes that occur are the loss of alveolar structure and airspace enlargement. To treat COPD, it is crucial to repair damaged lung tissue and regenerate the lost alveoli. Type II alveolar epithelial cells (AECII) play a vital role in maintaining lung tissue repair, and amniotic fluid-derived mesenchymal stromal cells (AFMSCs) possess the characteristics of regular mesenchymal stromal cells. However, it remains untested whether transplantation of rat AFMSCs (rAFMSCs) might alleviate lung injury caused by emphysema by increasing the expression of surfactant protein (SP)A and SPC and inhibiting AECII apoptosis.

Methods: We analyzed the phenotypic characteristics, differentiation potential, and karyotype of rAFMSCs, which were isolated from pregnant Sprague-Dawley rats. Moreover, we examined the lung morphology and the expression levels of SPA and SPC in rats with emphysema after cigarette-smoke exposure and intratracheal lipopolysaccharide instillation and rAFMSC transplantation. The ability of rAFMSCs to differentiate was measured, and the apoptosis of AECII was evaluated.

Results: In rAFMSCs, the surface antigens CD29, CD44, CD73, CD90, CD105, and CD166 were expressed, but CD14, CD19, CD34, and CD45 were not detected; rAFMSCs also strongly expressed the mRNA of octamer-binding transcription factor 4, and the cells could be induced to differentiate into adipocytes and osteocytes. Furthermore, rAFMSC treatment up-regulated the levels of SPA, SPC, and thyroid transcription factor 1 and inhibited AECII apoptosis, and rAFMSCs appeared to be capable of differentiating into AECII-like cells. Lung injury caused by emphysema was alleviated after rAFMSC treatment.

Conclusions: rAFMSCs might differentiate into AECII-like cells or induce local regeneration of the lung alveolar epithelium in vivo after transplantation and thus could be used in COPD treatment and lung regenerative therapy.

Show MeSH
Related in: MedlinePlus