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Anti-proliferative of physcion 8-O-β-glucopyranoside isolated from Rumex japonicus Houtt. on A549 cell lines via inducing apoptosis and cell cycle arrest.

Xie QC, Yang YP - BMC Complement Altern Med (2014)

Bottom Line: From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner.Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression.In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, the Second Affiliated Hospital, Third Military Medical University, 183 Xinqiao main street, Chongqing 400037, China. xieqctmmu@126.com.

ABSTRACT

Background: Lung cancers are leading causes of cancer death, and Rumex japonicus has been traditionally used in folk medicine as anti-microorganic, anti-inflammatory and anti-tumor agents. This study was designed to investigate the anti-proliferative activity of physcion 8-O-β-glucopyranoside (PG) isolated from Rumex japonicus Houtt. on A549 cell lines.

Methods: In our present study, PG was isolated and identified from the ethanol extracts of R. japonicus. MTT method was used to evaluate the anti-proliferative activity of PG on A549 cell lines, and cell cycle distribution assay, apoptosis assay, and western blot analysis in vitro were used to explore the possible mechanisms.

Results: From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner. Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression. In addition, the results of apoptosis assay and western blot analysis indicated that the anti-proliferative activity could be related to apoptosis via up-regulating the expressions of Bax, caspase-3 and caspase-7, and down-regulating the expressions of Bcl-2.

Conclusions: In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

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Related in: MedlinePlus

PG inhibits the viability of A549 cells in dose- and time-dependent manners. In the figure, Physcion 8-O-β-glucopyranoside abbreviated as PG. Cells were seeded in 96-well plates and treated on the following day with indicated concentrations of PG for 24 and 48 h, respectively, the cells viabilities were determined by MTT assay.
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Fig2: PG inhibits the viability of A549 cells in dose- and time-dependent manners. In the figure, Physcion 8-O-β-glucopyranoside abbreviated as PG. Cells were seeded in 96-well plates and treated on the following day with indicated concentrations of PG for 24 and 48 h, respectively, the cells viabilities were determined by MTT assay.

Mentions: To evaluate the cytotoxic effect of PG on A549 cell and get the IC50 value, the viability of cells treated with different concentrations (ranging from 5 to 80 μg/mL) of PG was measured using MTT test. The result showed that PG reduced cell survival in a dose- and time-dependent manner (Figure 2). The IC50 of PG to A549 cell lines was 53.01 μg/mL at 24 h and 27.31 μg/mL at 48 h, respectively. Based on the results, the concentration of 20, 40 and 80 μg/mL were selected and used in the subsequent experiment.Figure 2


Anti-proliferative of physcion 8-O-β-glucopyranoside isolated from Rumex japonicus Houtt. on A549 cell lines via inducing apoptosis and cell cycle arrest.

Xie QC, Yang YP - BMC Complement Altern Med (2014)

PG inhibits the viability of A549 cells in dose- and time-dependent manners. In the figure, Physcion 8-O-β-glucopyranoside abbreviated as PG. Cells were seeded in 96-well plates and treated on the following day with indicated concentrations of PG for 24 and 48 h, respectively, the cells viabilities were determined by MTT assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4201690&req=5

Fig2: PG inhibits the viability of A549 cells in dose- and time-dependent manners. In the figure, Physcion 8-O-β-glucopyranoside abbreviated as PG. Cells were seeded in 96-well plates and treated on the following day with indicated concentrations of PG for 24 and 48 h, respectively, the cells viabilities were determined by MTT assay.
Mentions: To evaluate the cytotoxic effect of PG on A549 cell and get the IC50 value, the viability of cells treated with different concentrations (ranging from 5 to 80 μg/mL) of PG was measured using MTT test. The result showed that PG reduced cell survival in a dose- and time-dependent manner (Figure 2). The IC50 of PG to A549 cell lines was 53.01 μg/mL at 24 h and 27.31 μg/mL at 48 h, respectively. Based on the results, the concentration of 20, 40 and 80 μg/mL were selected and used in the subsequent experiment.Figure 2

Bottom Line: From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner.Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression.In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, the Second Affiliated Hospital, Third Military Medical University, 183 Xinqiao main street, Chongqing 400037, China. xieqctmmu@126.com.

ABSTRACT

Background: Lung cancers are leading causes of cancer death, and Rumex japonicus has been traditionally used in folk medicine as anti-microorganic, anti-inflammatory and anti-tumor agents. This study was designed to investigate the anti-proliferative activity of physcion 8-O-β-glucopyranoside (PG) isolated from Rumex japonicus Houtt. on A549 cell lines.

Methods: In our present study, PG was isolated and identified from the ethanol extracts of R. japonicus. MTT method was used to evaluate the anti-proliferative activity of PG on A549 cell lines, and cell cycle distribution assay, apoptosis assay, and western blot analysis in vitro were used to explore the possible mechanisms.

Results: From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner. Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression. In addition, the results of apoptosis assay and western blot analysis indicated that the anti-proliferative activity could be related to apoptosis via up-regulating the expressions of Bax, caspase-3 and caspase-7, and down-regulating the expressions of Bcl-2.

Conclusions: In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

Show MeSH
Related in: MedlinePlus