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Anti-proliferative of physcion 8-O-β-glucopyranoside isolated from Rumex japonicus Houtt. on A549 cell lines via inducing apoptosis and cell cycle arrest.

Xie QC, Yang YP - BMC Complement Altern Med (2014)

Bottom Line: From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner.Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression.In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, the Second Affiliated Hospital, Third Military Medical University, 183 Xinqiao main street, Chongqing 400037, China. xieqctmmu@126.com.

ABSTRACT

Background: Lung cancers are leading causes of cancer death, and Rumex japonicus has been traditionally used in folk medicine as anti-microorganic, anti-inflammatory and anti-tumor agents. This study was designed to investigate the anti-proliferative activity of physcion 8-O-β-glucopyranoside (PG) isolated from Rumex japonicus Houtt. on A549 cell lines.

Methods: In our present study, PG was isolated and identified from the ethanol extracts of R. japonicus. MTT method was used to evaluate the anti-proliferative activity of PG on A549 cell lines, and cell cycle distribution assay, apoptosis assay, and western blot analysis in vitro were used to explore the possible mechanisms.

Results: From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner. Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression. In addition, the results of apoptosis assay and western blot analysis indicated that the anti-proliferative activity could be related to apoptosis via up-regulating the expressions of Bax, caspase-3 and caspase-7, and down-regulating the expressions of Bcl-2.

Conclusions: In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

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Molecular structure of physcion 8-O-β-glucopyranoside.
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Fig1: Molecular structure of physcion 8-O-β-glucopyranoside.

Mentions: The compound isolated from the Rumex japonicus Houtt. was identified as physcion 8-O-β-glucopyranoside[16, 17] (Figure 1, Table 2). The spectral data of the compound were as follows: Yellow needle crystals; melting point (m.p.) 231-233°C; electrospray ionisation tandem mass spectrometry (ESI-MS) m/z: 445.1 [M - H]-, and it showed the molecular ion at m/z 446, which was in accordance with the molecular formula C22H22O10. The 1H-NMR and 13C-NMR information was shown in Table 2.Figure 1


Anti-proliferative of physcion 8-O-β-glucopyranoside isolated from Rumex japonicus Houtt. on A549 cell lines via inducing apoptosis and cell cycle arrest.

Xie QC, Yang YP - BMC Complement Altern Med (2014)

Molecular structure of physcion 8-O-β-glucopyranoside.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4201690&req=5

Fig1: Molecular structure of physcion 8-O-β-glucopyranoside.
Mentions: The compound isolated from the Rumex japonicus Houtt. was identified as physcion 8-O-β-glucopyranoside[16, 17] (Figure 1, Table 2). The spectral data of the compound were as follows: Yellow needle crystals; melting point (m.p.) 231-233°C; electrospray ionisation tandem mass spectrometry (ESI-MS) m/z: 445.1 [M - H]-, and it showed the molecular ion at m/z 446, which was in accordance with the molecular formula C22H22O10. The 1H-NMR and 13C-NMR information was shown in Table 2.Figure 1

Bottom Line: From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner.Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression.In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, the Second Affiliated Hospital, Third Military Medical University, 183 Xinqiao main street, Chongqing 400037, China. xieqctmmu@126.com.

ABSTRACT

Background: Lung cancers are leading causes of cancer death, and Rumex japonicus has been traditionally used in folk medicine as anti-microorganic, anti-inflammatory and anti-tumor agents. This study was designed to investigate the anti-proliferative activity of physcion 8-O-β-glucopyranoside (PG) isolated from Rumex japonicus Houtt. on A549 cell lines.

Methods: In our present study, PG was isolated and identified from the ethanol extracts of R. japonicus. MTT method was used to evaluate the anti-proliferative activity of PG on A549 cell lines, and cell cycle distribution assay, apoptosis assay, and western blot analysis in vitro were used to explore the possible mechanisms.

Results: From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner. Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression. In addition, the results of apoptosis assay and western blot analysis indicated that the anti-proliferative activity could be related to apoptosis via up-regulating the expressions of Bax, caspase-3 and caspase-7, and down-regulating the expressions of Bcl-2.

Conclusions: In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

Show MeSH
Related in: MedlinePlus