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Expression levels of vascular endothelial growth factors a and C in patients with peptic ulcers and gastric cancer.

Taghizadeh S, Sankian M, Ajami A, Tehrani M, Hafezi N, Mohammadian R, Farazmandfar T, Hosseini V, Abbasi A, Ajami M - J Gastric Cancer (2014)

Bottom Line: We also analyzed the effect of Helicobacter pylori infection on VEGF-A and C expression levels.The VEGF-C expression level in the PUD and GC groups was significantly higher than that in the NUD group.In addition, the expression levels of these two molecules were higher in H. pylori positive patients with PUD or GC than in H. pylori negative patients of the same groups; however, these differences did not reach statistical significance.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Purpose: Vascular endothelial growth factor (VEGF) is one of the most important growth factors for metastatic tumors. To clarify the role of VEGF-A and C in patients with peptic ulcer disease (PUD) or gastric cancer (GC), we evaluated the expression levels of these two molecules. We also analyzed the effect of Helicobacter pylori infection on VEGF-A and C expression levels.

Materials and methods: PATIENTS WITH DYSPEPSIA WHO NEEDED DIAGNOSTIC ENDOSCOPY WERE SELECTED AND DIVIDED INTO THREE GROUPS: non-ulcer dyspepsia (NUD), PUD, and GC, according to their endoscopic and histopathological results. Fifty-two patients with NUD, 50 with PUD, and 38 with GC were enrolled in this study. H. pylori infection was diagnosed by the rapid urease test. After RNA extraction and synthesis of cDNA, the expression levels of VEGF-A and C were determined by quantitative reverse transcriptase polymerase chain reaction.

Results: The VEGF-C expression level in the PUD and GC groups was significantly higher than that in the NUD group. Moreover, the VEGF-A expression level in the PUD and GC groups was higher than in the NUD group, although the differences were not statistically significant. Significant positive correlations were also observed between the expression levels of these two molecules in the PUD and GC groups. In addition, the expression levels of these two molecules were higher in H. pylori positive patients with PUD or GC than in H. pylori negative patients of the same groups; however, these differences did not reach statistical significance.

Conclusions: Up-regulation of VEGF-C expression during gastric mucosal inflammation may play a role in the development of peptic ulcers or GC.

No MeSH data available.


Related in: MedlinePlus

Relative gene expression levels (2-ΔΔCt) of VEGF-A and VEGF-C in patients with NUD, PUD, and GC. *Denotes significant differences between groups. VEGF = vascular endothelial growth factor; NUD = non-ulcer dyspepsia; PUD = peptic ulcer disease; GC = gastric cancer.
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Figure 1: Relative gene expression levels (2-ΔΔCt) of VEGF-A and VEGF-C in patients with NUD, PUD, and GC. *Denotes significant differences between groups. VEGF = vascular endothelial growth factor; NUD = non-ulcer dyspepsia; PUD = peptic ulcer disease; GC = gastric cancer.

Mentions: Gene expression levels of VEGF-C were determined using qRT-PCR and normalized to the expression level of HGPRT for each individual sample. The results show that the relative expression levels of VEGF-C were higher in GC and PUD patients than in NUD patients (P<0.000 and P<0.000, respectively; Table 3, Fig. 1).


Expression levels of vascular endothelial growth factors a and C in patients with peptic ulcers and gastric cancer.

Taghizadeh S, Sankian M, Ajami A, Tehrani M, Hafezi N, Mohammadian R, Farazmandfar T, Hosseini V, Abbasi A, Ajami M - J Gastric Cancer (2014)

Relative gene expression levels (2-ΔΔCt) of VEGF-A and VEGF-C in patients with NUD, PUD, and GC. *Denotes significant differences between groups. VEGF = vascular endothelial growth factor; NUD = non-ulcer dyspepsia; PUD = peptic ulcer disease; GC = gastric cancer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199887&req=5

Figure 1: Relative gene expression levels (2-ΔΔCt) of VEGF-A and VEGF-C in patients with NUD, PUD, and GC. *Denotes significant differences between groups. VEGF = vascular endothelial growth factor; NUD = non-ulcer dyspepsia; PUD = peptic ulcer disease; GC = gastric cancer.
Mentions: Gene expression levels of VEGF-C were determined using qRT-PCR and normalized to the expression level of HGPRT for each individual sample. The results show that the relative expression levels of VEGF-C were higher in GC and PUD patients than in NUD patients (P<0.000 and P<0.000, respectively; Table 3, Fig. 1).

Bottom Line: We also analyzed the effect of Helicobacter pylori infection on VEGF-A and C expression levels.The VEGF-C expression level in the PUD and GC groups was significantly higher than that in the NUD group.In addition, the expression levels of these two molecules were higher in H. pylori positive patients with PUD or GC than in H. pylori negative patients of the same groups; however, these differences did not reach statistical significance.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Purpose: Vascular endothelial growth factor (VEGF) is one of the most important growth factors for metastatic tumors. To clarify the role of VEGF-A and C in patients with peptic ulcer disease (PUD) or gastric cancer (GC), we evaluated the expression levels of these two molecules. We also analyzed the effect of Helicobacter pylori infection on VEGF-A and C expression levels.

Materials and methods: PATIENTS WITH DYSPEPSIA WHO NEEDED DIAGNOSTIC ENDOSCOPY WERE SELECTED AND DIVIDED INTO THREE GROUPS: non-ulcer dyspepsia (NUD), PUD, and GC, according to their endoscopic and histopathological results. Fifty-two patients with NUD, 50 with PUD, and 38 with GC were enrolled in this study. H. pylori infection was diagnosed by the rapid urease test. After RNA extraction and synthesis of cDNA, the expression levels of VEGF-A and C were determined by quantitative reverse transcriptase polymerase chain reaction.

Results: The VEGF-C expression level in the PUD and GC groups was significantly higher than that in the NUD group. Moreover, the VEGF-A expression level in the PUD and GC groups was higher than in the NUD group, although the differences were not statistically significant. Significant positive correlations were also observed between the expression levels of these two molecules in the PUD and GC groups. In addition, the expression levels of these two molecules were higher in H. pylori positive patients with PUD or GC than in H. pylori negative patients of the same groups; however, these differences did not reach statistical significance.

Conclusions: Up-regulation of VEGF-C expression during gastric mucosal inflammation may play a role in the development of peptic ulcers or GC.

No MeSH data available.


Related in: MedlinePlus