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The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis.

Yang PS, Hsu YC, Lin YH, Hou CP, Chen CL, Chang PL, Juang HH, Tsui KH - Onco Targets Ther (2014)

Bottom Line: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value.ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4).ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chang Gung Memorial Hospital at Linkou, Taiwan, Republic of China.

ABSTRACT

Purpose: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value.

Patients and methods: From 2004 to 2009, eleven patients were enrolled in this study: six with high-grade bladder carcinoma and five with low-grade bladder carcinoma. ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4). Immunohistochemistry and real-time reverse transcription-polymerase chain reaction were used to validate the degree of EST expression and the prognostic value of ESTs.

Results: Apoferritin H subunit (FTH1 protein) exhibited increased expression in high-grade bladder carcinoma compared with that seen in low-grade carcinoma. Immunohistochemistry and real-time reverse transcription-polymerase chain reaction both supported the higher expression of FTH1 in high-grade urothelial carcinoma.

Conclusion: ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma.

No MeSH data available.


Related in: MedlinePlus

Reverse transcriptase-polymerase chain reaction analysis of tissue samples from six patients with high-grade TCC and five patients with low-grade TCC showed that FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (P=0.0122).Notes: (A) Reverse transcriptase-polymerase chain reaction analysis of tissue samples from six patients with high-grade TCC and five patients with low-grade TCC. (B) The RT-PCR showed that FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (P=0.0122).Abbreviations: FTH1, apoferritin H subunit; TH, high-grade tumor; TL, low-grade tumor; dRn, delta ribonucleotide; TCC, transitional cell carcinoma.
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f2-ott-7-1777: Reverse transcriptase-polymerase chain reaction analysis of tissue samples from six patients with high-grade TCC and five patients with low-grade TCC showed that FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (P=0.0122).Notes: (A) Reverse transcriptase-polymerase chain reaction analysis of tissue samples from six patients with high-grade TCC and five patients with low-grade TCC. (B) The RT-PCR showed that FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (P=0.0122).Abbreviations: FTH1, apoferritin H subunit; TH, high-grade tumor; TL, low-grade tumor; dRn, delta ribonucleotide; TCC, transitional cell carcinoma.

Mentions: To evaluate the relationship between FTH1 expression and degree of malignancy, we used real-time reverse transcription-polymerase chain reaction to calculate FTH1 mRNA expression in patient tissue samples. FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (mean expression, 58.67±6.125 versus 24.60±9.453, respectively; P=0.0122; Figure 2).


The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis.

Yang PS, Hsu YC, Lin YH, Hou CP, Chen CL, Chang PL, Juang HH, Tsui KH - Onco Targets Ther (2014)

Reverse transcriptase-polymerase chain reaction analysis of tissue samples from six patients with high-grade TCC and five patients with low-grade TCC showed that FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (P=0.0122).Notes: (A) Reverse transcriptase-polymerase chain reaction analysis of tissue samples from six patients with high-grade TCC and five patients with low-grade TCC. (B) The RT-PCR showed that FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (P=0.0122).Abbreviations: FTH1, apoferritin H subunit; TH, high-grade tumor; TL, low-grade tumor; dRn, delta ribonucleotide; TCC, transitional cell carcinoma.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199822&req=5

f2-ott-7-1777: Reverse transcriptase-polymerase chain reaction analysis of tissue samples from six patients with high-grade TCC and five patients with low-grade TCC showed that FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (P=0.0122).Notes: (A) Reverse transcriptase-polymerase chain reaction analysis of tissue samples from six patients with high-grade TCC and five patients with low-grade TCC. (B) The RT-PCR showed that FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (P=0.0122).Abbreviations: FTH1, apoferritin H subunit; TH, high-grade tumor; TL, low-grade tumor; dRn, delta ribonucleotide; TCC, transitional cell carcinoma.
Mentions: To evaluate the relationship between FTH1 expression and degree of malignancy, we used real-time reverse transcription-polymerase chain reaction to calculate FTH1 mRNA expression in patient tissue samples. FTH1 was expressed at higher levels in patients with high-grade TCC than in patients with low-grade TCC (mean expression, 58.67±6.125 versus 24.60±9.453, respectively; P=0.0122; Figure 2).

Bottom Line: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value.ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4).ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chang Gung Memorial Hospital at Linkou, Taiwan, Republic of China.

ABSTRACT

Purpose: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value.

Patients and methods: From 2004 to 2009, eleven patients were enrolled in this study: six with high-grade bladder carcinoma and five with low-grade bladder carcinoma. ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4). Immunohistochemistry and real-time reverse transcription-polymerase chain reaction were used to validate the degree of EST expression and the prognostic value of ESTs.

Results: Apoferritin H subunit (FTH1 protein) exhibited increased expression in high-grade bladder carcinoma compared with that seen in low-grade carcinoma. Immunohistochemistry and real-time reverse transcription-polymerase chain reaction both supported the higher expression of FTH1 in high-grade urothelial carcinoma.

Conclusion: ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma.

No MeSH data available.


Related in: MedlinePlus