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Cell migration is regulated by AGE-RAGE interaction in human oral cancer cells in vitro.

Ko SY, Ko HA, Shieh TM, Chang WC, Chen HI, Chang SS, Lin IH - PLoS ONE (2014)

Bottom Line: In this study, AGEs increased ERK phosphorylation, enhanced cell migration, and promoted the expression of RAGE, MMP2, and MMP9.Using PD98059, RAGE antibody, and RAGE RNAi to block RAGE pathway resulted in the inhibition of ERK phosphorylation.Cell migration, MMP2 and MMP9 expression were also reduced by this treatment.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Medical Science, College of Health Science, Chang Jung Christian University, Tainan, Taiwan; Innovate Research Center of Medicine, Chang Jung Christian University, Tainan, Taiwan.

ABSTRACT
Advanced glycation end products (AGEs) are produced in an irreversible non-enzymatic reaction of carbohydrates and proteins. Patients with diabetes mellitus (DM) are known to have elevated AGE levels, which is viewed as a risk factor of diabetes-related complications. In a clinical setting, it has been shown that patients with oral cancer in conjunction with DM have a higher likelihood of cancer metastasis and lower cancer survival rates. AGE-RAGE (a receptor of AGEs) is also correlated with metastasis and angiogenesis. Recent studies have suggested that the malignancy of cancer may be enhanced by glyceraldehyde-derived AGEs; however, the underlying mechanism remains unclear. This study examined the apparently close correlation between AGE-RAGE and the malignancy of SAS oral cancer cell line. In this study, AGEs increased ERK phosphorylation, enhanced cell migration, and promoted the expression of RAGE, MMP2, and MMP9. Using PD98059, RAGE antibody, and RAGE RNAi to block RAGE pathway resulted in the inhibition of ERK phosphorylation. Cell migration, MMP2 and MMP9 expression were also reduced by this treatment. Our findings demonstrate the importance of AGE-RAGE with regard to the malignancy of oral cancer, and help to explain the poor prognosis of DM subjects with oral cancer.

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Related in: MedlinePlus

AGEs regulated RAGE, MMP2, and MMP9.After treating cells with AGEs (200 and 400 µg/ml) or BSA (400 µg/ml) for 24 hours, RAGE, MMP2, and MMP9 were detected by western blot analysis (A). AGEs significantly increased the expression of RAGE, MMP2, and MMP9 (B).
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pone-0110542-g002: AGEs regulated RAGE, MMP2, and MMP9.After treating cells with AGEs (200 and 400 µg/ml) or BSA (400 µg/ml) for 24 hours, RAGE, MMP2, and MMP9 were detected by western blot analysis (A). AGEs significantly increased the expression of RAGE, MMP2, and MMP9 (B).

Mentions: Cells were treated with AGEs (200 and 400 µg/ml) or BSA (400 µg/ml) for 24 hours. We then used western blot analysis to detect RAGE, MMP2, and MMP9. Compared with control cells, the result showed that AGEs treated cells presented a significant increase in RAGE (AGEs 400: 1.3±0.03, P = 0.0007), MMP2 (AGEs 200: 1.28±0.04, P = 0.002; AGEs 400: 1.47±0.04, P = 0.004), and MMP9 (AGEs 400: 1.24±0.03, P = 0.0008) (Fig. 2). Moreover, the functionality of MMP2 and MMP9 was increased after treatment with AGEs for 4 or 24 hours (Fig S2).


Cell migration is regulated by AGE-RAGE interaction in human oral cancer cells in vitro.

Ko SY, Ko HA, Shieh TM, Chang WC, Chen HI, Chang SS, Lin IH - PLoS ONE (2014)

AGEs regulated RAGE, MMP2, and MMP9.After treating cells with AGEs (200 and 400 µg/ml) or BSA (400 µg/ml) for 24 hours, RAGE, MMP2, and MMP9 were detected by western blot analysis (A). AGEs significantly increased the expression of RAGE, MMP2, and MMP9 (B).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199749&req=5

pone-0110542-g002: AGEs regulated RAGE, MMP2, and MMP9.After treating cells with AGEs (200 and 400 µg/ml) or BSA (400 µg/ml) for 24 hours, RAGE, MMP2, and MMP9 were detected by western blot analysis (A). AGEs significantly increased the expression of RAGE, MMP2, and MMP9 (B).
Mentions: Cells were treated with AGEs (200 and 400 µg/ml) or BSA (400 µg/ml) for 24 hours. We then used western blot analysis to detect RAGE, MMP2, and MMP9. Compared with control cells, the result showed that AGEs treated cells presented a significant increase in RAGE (AGEs 400: 1.3±0.03, P = 0.0007), MMP2 (AGEs 200: 1.28±0.04, P = 0.002; AGEs 400: 1.47±0.04, P = 0.004), and MMP9 (AGEs 400: 1.24±0.03, P = 0.0008) (Fig. 2). Moreover, the functionality of MMP2 and MMP9 was increased after treatment with AGEs for 4 or 24 hours (Fig S2).

Bottom Line: In this study, AGEs increased ERK phosphorylation, enhanced cell migration, and promoted the expression of RAGE, MMP2, and MMP9.Using PD98059, RAGE antibody, and RAGE RNAi to block RAGE pathway resulted in the inhibition of ERK phosphorylation.Cell migration, MMP2 and MMP9 expression were also reduced by this treatment.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Medical Science, College of Health Science, Chang Jung Christian University, Tainan, Taiwan; Innovate Research Center of Medicine, Chang Jung Christian University, Tainan, Taiwan.

ABSTRACT
Advanced glycation end products (AGEs) are produced in an irreversible non-enzymatic reaction of carbohydrates and proteins. Patients with diabetes mellitus (DM) are known to have elevated AGE levels, which is viewed as a risk factor of diabetes-related complications. In a clinical setting, it has been shown that patients with oral cancer in conjunction with DM have a higher likelihood of cancer metastasis and lower cancer survival rates. AGE-RAGE (a receptor of AGEs) is also correlated with metastasis and angiogenesis. Recent studies have suggested that the malignancy of cancer may be enhanced by glyceraldehyde-derived AGEs; however, the underlying mechanism remains unclear. This study examined the apparently close correlation between AGE-RAGE and the malignancy of SAS oral cancer cell line. In this study, AGEs increased ERK phosphorylation, enhanced cell migration, and promoted the expression of RAGE, MMP2, and MMP9. Using PD98059, RAGE antibody, and RAGE RNAi to block RAGE pathway resulted in the inhibition of ERK phosphorylation. Cell migration, MMP2 and MMP9 expression were also reduced by this treatment. Our findings demonstrate the importance of AGE-RAGE with regard to the malignancy of oral cancer, and help to explain the poor prognosis of DM subjects with oral cancer.

Show MeSH
Related in: MedlinePlus