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Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.

Liu H, Cheng Z, Song W, Wu W, Zhou Z - PLoS ONE (2014)

Bottom Line: Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost.Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified.Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu).

View Article: PubMed Central - PubMed

Affiliation: Department of Critical Care, First Affiliated Hospital of Anhui Medical University, Anhui, Hefei, P.R. China.

ABSTRACT
Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia.

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Western blot analysis to validate the reactive proteins.Klebsiella pneumonia from Klebsiella pneumonia bacteremia patients with (A) leukopenia and (B) leukocytosis were respectively examined by Western blot analysis to validate the reactive proteins ef-tu. Note the higher expression levels of ef-tu in patients with leukopenia caused by Klebsiella pneumonia.
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pone-0110011-g002: Western blot analysis to validate the reactive proteins.Klebsiella pneumonia from Klebsiella pneumonia bacteremia patients with (A) leukopenia and (B) leukocytosis were respectively examined by Western blot analysis to validate the reactive proteins ef-tu. Note the higher expression levels of ef-tu in patients with leukopenia caused by Klebsiella pneumonia.

Mentions: To confirm the immunoproteomic data, another 3 Klebsiella pneumonia isolates from patients with bacteremia and leukopenia were compared with another 3 Klebsiella pneumonia isolates from patients with leukocytosis by Western blot. Drug-susceptible isolates with bacteremia and leukopenia had high levels of elongation factor tu expression (Fig 2A, Fig 2B).


Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.

Liu H, Cheng Z, Song W, Wu W, Zhou Z - PLoS ONE (2014)

Western blot analysis to validate the reactive proteins.Klebsiella pneumonia from Klebsiella pneumonia bacteremia patients with (A) leukopenia and (B) leukocytosis were respectively examined by Western blot analysis to validate the reactive proteins ef-tu. Note the higher expression levels of ef-tu in patients with leukopenia caused by Klebsiella pneumonia.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199714&req=5

pone-0110011-g002: Western blot analysis to validate the reactive proteins.Klebsiella pneumonia from Klebsiella pneumonia bacteremia patients with (A) leukopenia and (B) leukocytosis were respectively examined by Western blot analysis to validate the reactive proteins ef-tu. Note the higher expression levels of ef-tu in patients with leukopenia caused by Klebsiella pneumonia.
Mentions: To confirm the immunoproteomic data, another 3 Klebsiella pneumonia isolates from patients with bacteremia and leukopenia were compared with another 3 Klebsiella pneumonia isolates from patients with leukocytosis by Western blot. Drug-susceptible isolates with bacteremia and leukopenia had high levels of elongation factor tu expression (Fig 2A, Fig 2B).

Bottom Line: Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost.Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified.Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu).

View Article: PubMed Central - PubMed

Affiliation: Department of Critical Care, First Affiliated Hospital of Anhui Medical University, Anhui, Hefei, P.R. China.

ABSTRACT
Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia.

Show MeSH
Related in: MedlinePlus