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Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.

Liu H, Cheng Z, Song W, Wu W, Zhou Z - PLoS ONE (2014)

Bottom Line: Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost.Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified.Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu).

View Article: PubMed Central - PubMed

Affiliation: Department of Critical Care, First Affiliated Hospital of Anhui Medical University, Anhui, Hefei, P.R. China.

ABSTRACT
Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia.

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2-DE analysis of proteins from Klebsiella pneumonia in leukocyte patients caused by Klebsiella pneumonia bacteremia.Detection of pathogenicity factors by Western blot analysis in CD patients. The six protein spots are labeled with arrows.
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pone-0110011-g001: 2-DE analysis of proteins from Klebsiella pneumonia in leukocyte patients caused by Klebsiella pneumonia bacteremia.Detection of pathogenicity factors by Western blot analysis in CD patients. The six protein spots are labeled with arrows.

Mentions: Proteins of interest were extracted from the gels after 2-D PAGE and silver staining. The proteins were digested with trypsin, chymotrypsin, and Glu-C, and the resulting peptides were analyzed by MALDI-TOF MS. The corresponding spectra were used to identify the proteins with the Mascot search program. The reactive proteins were identified as S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor Tu (Fig. 1, Table 1).


Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.

Liu H, Cheng Z, Song W, Wu W, Zhou Z - PLoS ONE (2014)

2-DE analysis of proteins from Klebsiella pneumonia in leukocyte patients caused by Klebsiella pneumonia bacteremia.Detection of pathogenicity factors by Western blot analysis in CD patients. The six protein spots are labeled with arrows.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199714&req=5

pone-0110011-g001: 2-DE analysis of proteins from Klebsiella pneumonia in leukocyte patients caused by Klebsiella pneumonia bacteremia.Detection of pathogenicity factors by Western blot analysis in CD patients. The six protein spots are labeled with arrows.
Mentions: Proteins of interest were extracted from the gels after 2-D PAGE and silver staining. The proteins were digested with trypsin, chymotrypsin, and Glu-C, and the resulting peptides were analyzed by MALDI-TOF MS. The corresponding spectra were used to identify the proteins with the Mascot search program. The reactive proteins were identified as S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor Tu (Fig. 1, Table 1).

Bottom Line: Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost.Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified.Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu).

View Article: PubMed Central - PubMed

Affiliation: Department of Critical Care, First Affiliated Hospital of Anhui Medical University, Anhui, Hefei, P.R. China.

ABSTRACT
Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia.

Show MeSH
Related in: MedlinePlus