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The alternative oxidase AOX does not rescue the phenotype of tko25t mutant flies.

Kemppainen KK, Kemppainen E, Jacobs HT - G3 (Bethesda) (2014)

Bottom Line: AOX expression for 1 d after eclosion, or continuously throughout development, had no effect on the bang sensitivity of tko(25t) adults, and continued expression in adults older than 30 d also produced no amelioration of the phenotype.We conclude that AOX does not rescue tko(25t) and that the mutant phenotype is not solely due to limitations on electron flow in the respiratory chain, but rather to a more complex metabolic defect.The future therapeutic use of AOX in disorders of mitochondrial translation may thus be of limited value.

View Article: PubMed Central - PubMed

Affiliation: BioMediTech and Tampere University Hospital, FI-33014 University of Tampere, Tampere, Finland.

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Phenotype of tko25t is unaffected by AOX expression throughout development. (A) Eclosion day and (B) bang sensitivity of 1-d-old flies of the sex and genotype indicated, cultured throughout development on medium with or without 10 μM RU486. Means ± SD based on eclosion data from three replicate experiments and bang sensitivity of groups of 50 individually analyzed flies.
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fig3: Phenotype of tko25t is unaffected by AOX expression throughout development. (A) Eclosion day and (B) bang sensitivity of 1-d-old flies of the sex and genotype indicated, cultured throughout development on medium with or without 10 μM RU486. Means ± SD based on eclosion data from three replicate experiments and bang sensitivity of groups of 50 individually analyzed flies.

Mentions: Once again, we observed no rescue of the mutant phenotype that was attributable to AOX expression (Figure 3). The developmental delay of tko25t mutant flies (Figure 3A) was slightly greater in males than in females, as observed previously (Kemppainen et al. 2009), and an additional delay of approximately 1 d was produced in flies of all genotypes and both sexes by the presence of RU486 in the food. The UAS-AOX transgene, the tub-GS driver, and the two in combination did not produce any significant change in developmental timing of tko25t mutant flies, although there was a slight delay produced by AOX expression in wild-type flies, as reported previously using the da-GAL4 driver. The bang sensitivity of the progeny flies showed no significant change according to any of the parameters tested, except for the presence of the tko25t mutation itself (Figure 3B).


The alternative oxidase AOX does not rescue the phenotype of tko25t mutant flies.

Kemppainen KK, Kemppainen E, Jacobs HT - G3 (Bethesda) (2014)

Phenotype of tko25t is unaffected by AOX expression throughout development. (A) Eclosion day and (B) bang sensitivity of 1-d-old flies of the sex and genotype indicated, cultured throughout development on medium with or without 10 μM RU486. Means ± SD based on eclosion data from three replicate experiments and bang sensitivity of groups of 50 individually analyzed flies.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199707&req=5

fig3: Phenotype of tko25t is unaffected by AOX expression throughout development. (A) Eclosion day and (B) bang sensitivity of 1-d-old flies of the sex and genotype indicated, cultured throughout development on medium with or without 10 μM RU486. Means ± SD based on eclosion data from three replicate experiments and bang sensitivity of groups of 50 individually analyzed flies.
Mentions: Once again, we observed no rescue of the mutant phenotype that was attributable to AOX expression (Figure 3). The developmental delay of tko25t mutant flies (Figure 3A) was slightly greater in males than in females, as observed previously (Kemppainen et al. 2009), and an additional delay of approximately 1 d was produced in flies of all genotypes and both sexes by the presence of RU486 in the food. The UAS-AOX transgene, the tub-GS driver, and the two in combination did not produce any significant change in developmental timing of tko25t mutant flies, although there was a slight delay produced by AOX expression in wild-type flies, as reported previously using the da-GAL4 driver. The bang sensitivity of the progeny flies showed no significant change according to any of the parameters tested, except for the presence of the tko25t mutation itself (Figure 3B).

Bottom Line: AOX expression for 1 d after eclosion, or continuously throughout development, had no effect on the bang sensitivity of tko(25t) adults, and continued expression in adults older than 30 d also produced no amelioration of the phenotype.We conclude that AOX does not rescue tko(25t) and that the mutant phenotype is not solely due to limitations on electron flow in the respiratory chain, but rather to a more complex metabolic defect.The future therapeutic use of AOX in disorders of mitochondrial translation may thus be of limited value.

View Article: PubMed Central - PubMed

Affiliation: BioMediTech and Tampere University Hospital, FI-33014 University of Tampere, Tampere, Finland.

Show MeSH
Related in: MedlinePlus