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Genomic characterization of the LEED..PEEDs, a gene family unique to the medicago lineage.

Trujillo DI, Silverstein KA, Young ND - G3 (Bethesda) (2014)

Bottom Line: Synteny comparisons between R108 and A17 confirm that gene order is conserved between the two subspecies, although a further duplication occurred solely in A17.In M. truncatula A17, all 13 LPs are exclusively transcribed in nodules and absent from other plant tissues, including roots, leaves, flowers, seeds, seed shells, and pods.The recent expansion of LP genes in Medicago spp. and their timing and location of expression suggest a novel function in nodulation, possibly as an aftermath of the evolution of bacteroid terminal differentiation or potentially associated with rhizobial-host specificity.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108.

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Dot plot analysis of a ∼1-kbp region in chromosome 4 and ∼100-kbp region in chromosome 7 of M. truncatula R108 and A17. Black diagonal lines indicate duplicated regions within A17 (A) or R108 (B) or sequence colinearity between the two organisms (C). Red horizontal and vertical lines indicate the borders of duplicated and collinear regions.
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fig3: Dot plot analysis of a ∼1-kbp region in chromosome 4 and ∼100-kbp region in chromosome 7 of M. truncatula R108 and A17. Black diagonal lines indicate duplicated regions within A17 (A) or R108 (B) or sequence colinearity between the two organisms (C). Red horizontal and vertical lines indicate the borders of duplicated and collinear regions.

Mentions: A dotplot analysis (Figure 3) shows the location of duplications in the genomic regions surrounding LPs 2–13 in A17 and R108. The duplication of a region encompassing LP 2 gave rise to LP 3 in both A17 and R108. A more recent duplication of a region encompassing LP 3 and LP 6 occurred solely in the A17 lineage, giving rise to LP 4 and LP 5 (Figure 3A), which are absent from R108 (Figure 3B). Large regions of colinearity between the two subspecies are seen surrounding LPs 2–8, with a degradation of colinearity around LPs 9–13 (Figure 3C).


Genomic characterization of the LEED..PEEDs, a gene family unique to the medicago lineage.

Trujillo DI, Silverstein KA, Young ND - G3 (Bethesda) (2014)

Dot plot analysis of a ∼1-kbp region in chromosome 4 and ∼100-kbp region in chromosome 7 of M. truncatula R108 and A17. Black diagonal lines indicate duplicated regions within A17 (A) or R108 (B) or sequence colinearity between the two organisms (C). Red horizontal and vertical lines indicate the borders of duplicated and collinear regions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199706&req=5

fig3: Dot plot analysis of a ∼1-kbp region in chromosome 4 and ∼100-kbp region in chromosome 7 of M. truncatula R108 and A17. Black diagonal lines indicate duplicated regions within A17 (A) or R108 (B) or sequence colinearity between the two organisms (C). Red horizontal and vertical lines indicate the borders of duplicated and collinear regions.
Mentions: A dotplot analysis (Figure 3) shows the location of duplications in the genomic regions surrounding LPs 2–13 in A17 and R108. The duplication of a region encompassing LP 2 gave rise to LP 3 in both A17 and R108. A more recent duplication of a region encompassing LP 3 and LP 6 occurred solely in the A17 lineage, giving rise to LP 4 and LP 5 (Figure 3A), which are absent from R108 (Figure 3B). Large regions of colinearity between the two subspecies are seen surrounding LPs 2–8, with a degradation of colinearity around LPs 9–13 (Figure 3C).

Bottom Line: Synteny comparisons between R108 and A17 confirm that gene order is conserved between the two subspecies, although a further duplication occurred solely in A17.In M. truncatula A17, all 13 LPs are exclusively transcribed in nodules and absent from other plant tissues, including roots, leaves, flowers, seeds, seed shells, and pods.The recent expansion of LP genes in Medicago spp. and their timing and location of expression suggest a novel function in nodulation, possibly as an aftermath of the evolution of bacteroid terminal differentiation or potentially associated with rhizobial-host specificity.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108.

Show MeSH
Related in: MedlinePlus