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Genomic characterization of the LEED..PEEDs, a gene family unique to the medicago lineage.

Trujillo DI, Silverstein KA, Young ND - G3 (Bethesda) (2014)

Bottom Line: Synteny comparisons between R108 and A17 confirm that gene order is conserved between the two subspecies, although a further duplication occurred solely in A17.In M. truncatula A17, all 13 LPs are exclusively transcribed in nodules and absent from other plant tissues, including roots, leaves, flowers, seeds, seed shells, and pods.The recent expansion of LP genes in Medicago spp. and their timing and location of expression suggest a novel function in nodulation, possibly as an aftermath of the evolution of bacteroid terminal differentiation or potentially associated with rhizobial-host specificity.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108.

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Synteny comparisons between LPs 1–13 chromosomal regions in M. truncatula A17 and corresponding regions in G. max and C. arietinum. Shaded bars indicate synteny between the A17 region surrounding LP 1 on chromosome 4 with G. max chromosomes 15 and 8 (A), and of the A17 chromosome 7 region surrounding LPs 2–13 with G. max chromosome 8 and C. arietinum scaffold 451 (B). LP genes are shown in red and the region containing them surrounded by red boxes. Neighboring genes that have also undergone tandem duplications are shown in purple, and nonduplicated neighboring genes are shown in green. (B) The ∼93-kbp LP 2–13 region of A17 chromosome 7 is magnified and compared against a ∼100-kbp region of Scaffold 848 of R108. Shaded lines between chromosomes indicate syntenic regions.
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fig2: Synteny comparisons between LPs 1–13 chromosomal regions in M. truncatula A17 and corresponding regions in G. max and C. arietinum. Shaded bars indicate synteny between the A17 region surrounding LP 1 on chromosome 4 with G. max chromosomes 15 and 8 (A), and of the A17 chromosome 7 region surrounding LPs 2–13 with G. max chromosome 8 and C. arietinum scaffold 451 (B). LP genes are shown in red and the region containing them surrounded by red boxes. Neighboring genes that have also undergone tandem duplications are shown in purple, and nonduplicated neighboring genes are shown in green. (B) The ∼93-kbp LP 2–13 region of A17 chromosome 7 is magnified and compared against a ∼100-kbp region of Scaffold 848 of R108. Shaded lines between chromosomes indicate syntenic regions.

Mentions: In M. truncatula A17, LP 1 is located on chromosome 4, whereas LPs 2–13 are located in a 93-kbp region on chromosome 7. Neighboring regions of LP 1 on A17 chromosome 4 showed synteny with G. max chromosomes 8 and 15 (visualized in dotplot comparisons in Figure S4a and Figure S4b, respectively). Directly neighboring the LP 1 region on A17, an analysis of corresponding syntenic regions of G. max shows that LP 1 in A17 (Figure 2A, red arrow) is bordered by two sets of genes on either side that have multiple copies (purple arrows). An LP ortholog is not present within either of the syntenic genomic regions in G. max.


Genomic characterization of the LEED..PEEDs, a gene family unique to the medicago lineage.

Trujillo DI, Silverstein KA, Young ND - G3 (Bethesda) (2014)

Synteny comparisons between LPs 1–13 chromosomal regions in M. truncatula A17 and corresponding regions in G. max and C. arietinum. Shaded bars indicate synteny between the A17 region surrounding LP 1 on chromosome 4 with G. max chromosomes 15 and 8 (A), and of the A17 chromosome 7 region surrounding LPs 2–13 with G. max chromosome 8 and C. arietinum scaffold 451 (B). LP genes are shown in red and the region containing them surrounded by red boxes. Neighboring genes that have also undergone tandem duplications are shown in purple, and nonduplicated neighboring genes are shown in green. (B) The ∼93-kbp LP 2–13 region of A17 chromosome 7 is magnified and compared against a ∼100-kbp region of Scaffold 848 of R108. Shaded lines between chromosomes indicate syntenic regions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199706&req=5

fig2: Synteny comparisons between LPs 1–13 chromosomal regions in M. truncatula A17 and corresponding regions in G. max and C. arietinum. Shaded bars indicate synteny between the A17 region surrounding LP 1 on chromosome 4 with G. max chromosomes 15 and 8 (A), and of the A17 chromosome 7 region surrounding LPs 2–13 with G. max chromosome 8 and C. arietinum scaffold 451 (B). LP genes are shown in red and the region containing them surrounded by red boxes. Neighboring genes that have also undergone tandem duplications are shown in purple, and nonduplicated neighboring genes are shown in green. (B) The ∼93-kbp LP 2–13 region of A17 chromosome 7 is magnified and compared against a ∼100-kbp region of Scaffold 848 of R108. Shaded lines between chromosomes indicate syntenic regions.
Mentions: In M. truncatula A17, LP 1 is located on chromosome 4, whereas LPs 2–13 are located in a 93-kbp region on chromosome 7. Neighboring regions of LP 1 on A17 chromosome 4 showed synteny with G. max chromosomes 8 and 15 (visualized in dotplot comparisons in Figure S4a and Figure S4b, respectively). Directly neighboring the LP 1 region on A17, an analysis of corresponding syntenic regions of G. max shows that LP 1 in A17 (Figure 2A, red arrow) is bordered by two sets of genes on either side that have multiple copies (purple arrows). An LP ortholog is not present within either of the syntenic genomic regions in G. max.

Bottom Line: Synteny comparisons between R108 and A17 confirm that gene order is conserved between the two subspecies, although a further duplication occurred solely in A17.In M. truncatula A17, all 13 LPs are exclusively transcribed in nodules and absent from other plant tissues, including roots, leaves, flowers, seeds, seed shells, and pods.The recent expansion of LP genes in Medicago spp. and their timing and location of expression suggest a novel function in nodulation, possibly as an aftermath of the evolution of bacteroid terminal differentiation or potentially associated with rhizobial-host specificity.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108.

Show MeSH
Related in: MedlinePlus