Genomic characterization of the LEED..PEEDs, a gene family unique to the medicago lineage.
Bottom Line: Synteny comparisons between R108 and A17 confirm that gene order is conserved between the two subspecies, although a further duplication occurred solely in A17.In M. truncatula A17, all 13 LPs are exclusively transcribed in nodules and absent from other plant tissues, including roots, leaves, flowers, seeds, seed shells, and pods.The recent expansion of LP genes in Medicago spp. and their timing and location of expression suggest a novel function in nodulation, possibly as an aftermath of the evolution of bacteroid terminal differentiation or potentially associated with rhizobial-host specificity.
Affiliation: Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108.Show MeSH
Related in: MedlinePlus
Mentions: In M. truncatula A17, LP 1 is located on chromosome 4, whereas LPs 2–13 are located in a 93-kbp region on chromosome 7. Neighboring regions of LP 1 on A17 chromosome 4 showed synteny with G. max chromosomes 8 and 15 (visualized in dotplot comparisons in Figure S4a and Figure S4b, respectively). Directly neighboring the LP 1 region on A17, an analysis of corresponding syntenic regions of G. max shows that LP 1 in A17 (Figure 2A, red arrow) is bordered by two sets of genes on either side that have multiple copies (purple arrows). An LP ortholog is not present within either of the syntenic genomic regions in G. max.
Affiliation: Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108.