A saturated genetic linkage map of autotetraploid alfalfa (Medicago sativa L.) developed using genotyping-by-sequencing is highly syntenous with the Medicago truncatula genome.
Bottom Line: The alfalfa linkage groups were highly syntenous with M. truncatula, and clearly identified the known translocation between Chromosomes 4 and 8.In addition, a small inversion on Chromosome 1 was identified between M. truncatula and M. sativa.GBS could be used in breeding populations to accelerate molecular breeding in alfalfa.
Affiliation: Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401.Show MeSH
Related in: MedlinePlus
Mentions: The DM3 linkage map spanned a total of 2126 cM with a total of 1437 mapped markers and an average density of one marker per1.5 cM on each haplotype map (Table 2 and Figure 1). A total of 2154 markers were mapped on the DM5 linkage map with a total length of 2133 cM and an average density of one marker 1.0 cM per haplotype map (Table 2 and Figure 2). The number of markers varied from 21 to 91 among the 32 linkage groups for DM3 and from 20 to 189 for DM5 (Table 2).
Affiliation: Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401.