A saturated genetic linkage map of autotetraploid alfalfa (Medicago sativa L.) developed using genotyping-by-sequencing is highly syntenous with the Medicago truncatula genome.
Bottom Line: Four linkage groups representing the four haplotypes of each alfalfa chromosome were assigned to each of the eight Medicago chromosomes in both the maternal and paternal parents.The alfalfa linkage groups were highly syntenous with M. truncatula, and clearly identified the known translocation between Chromosomes 4 and 8.GBS could be used in breeding populations to accelerate molecular breeding in alfalfa.
Affiliation: Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401.Show MeSH
Related in: MedlinePlus
Mentions: The DM3 linkage map spanned a total of 2126 cM with a total of 1437 mapped markers and an average density of one marker per1.5 cM on each haplotype map (Table 2 and Figure 1). A total of 2154 markers were mapped on the DM5 linkage map with a total length of 2133 cM and an average density of one marker 1.0 cM per haplotype map (Table 2 and Figure 2). The number of markers varied from 21 to 91 among the 32 linkage groups for DM3 and from 20 to 189 for DM5 (Table 2).
Affiliation: Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401.