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Fine mapping and evolution of the major sex determining region in turbot (Scophthalmus maximus).

Taboada X, Hermida M, Pardo BG, Vera M, Piferrer F, Viñas A, Bouza C, Martínez P - G3 (Bethesda) (2014)

Bottom Line: Six genes were located to the SD region, two of them associated with gonad development (sox2 and dnajc19).Genetic diversity and differentiation for 25 LG5 genetic markers showed no differences between males and females sampled from a wild population, suggesting a recent origin of the SD region in turbot.We also analyzed associations with markers of the most relevant sex-related linkage groups in brill (S. rhombus), a closely related species to turbot; the data suggest that an ancient XX/XY system in brill changed to a ZZ/ZW mechanism in turbot.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética, Facultad de Biología (CIBUS), Universidad de Santiago de Compostela, Avda. Lope Gómez de Marzoa, 15782 Santiago de Compostela, Spain.

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Two-color fluorescence in situ hybridization (FISH) using bacterial artificial chromosome (BAC) clones at the main sex determination region of turbot (S. maximus). The fkbp2 and dlg1–bearing BAC clone (Sma51C11) and the ncbp2–bearing BAC clone (Sma58H5) were labeled with digoxigenin (red) and biotin (green), respectively. (A) Sma51C11; (B) Sma58H5; and (C) double-label BAC-FISH with Sma51C11 and Sma58H5.
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fig2: Two-color fluorescence in situ hybridization (FISH) using bacterial artificial chromosome (BAC) clones at the main sex determination region of turbot (S. maximus). The fkbp2 and dlg1–bearing BAC clone (Sma51C11) and the ncbp2–bearing BAC clone (Sma58H5) were labeled with digoxigenin (red) and biotin (green), respectively. (A) Sma51C11; (B) Sma58H5; and (C) double-label BAC-FISH with Sma51C11 and Sma58H5.

Mentions: The physical position of these genes was studied by BAC-FISH, including double fluorescence labeling. Two BAC clones (Sma51C11 and Sma58H5, around 100 kb each) were identified, one containing the fkbp2 and dlg1 genes (Sma51C11), and the other with ncbp2 (Sma58H5). Both probes cohybridized in the proximal region of a chromosome whose size and morphology correspond to LG5 (Taboada et al. 2014), supporting the genetic mapping (Figure 2).


Fine mapping and evolution of the major sex determining region in turbot (Scophthalmus maximus).

Taboada X, Hermida M, Pardo BG, Vera M, Piferrer F, Viñas A, Bouza C, Martínez P - G3 (Bethesda) (2014)

Two-color fluorescence in situ hybridization (FISH) using bacterial artificial chromosome (BAC) clones at the main sex determination region of turbot (S. maximus). The fkbp2 and dlg1–bearing BAC clone (Sma51C11) and the ncbp2–bearing BAC clone (Sma58H5) were labeled with digoxigenin (red) and biotin (green), respectively. (A) Sma51C11; (B) Sma58H5; and (C) double-label BAC-FISH with Sma51C11 and Sma58H5.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199694&req=5

fig2: Two-color fluorescence in situ hybridization (FISH) using bacterial artificial chromosome (BAC) clones at the main sex determination region of turbot (S. maximus). The fkbp2 and dlg1–bearing BAC clone (Sma51C11) and the ncbp2–bearing BAC clone (Sma58H5) were labeled with digoxigenin (red) and biotin (green), respectively. (A) Sma51C11; (B) Sma58H5; and (C) double-label BAC-FISH with Sma51C11 and Sma58H5.
Mentions: The physical position of these genes was studied by BAC-FISH, including double fluorescence labeling. Two BAC clones (Sma51C11 and Sma58H5, around 100 kb each) were identified, one containing the fkbp2 and dlg1 genes (Sma51C11), and the other with ncbp2 (Sma58H5). Both probes cohybridized in the proximal region of a chromosome whose size and morphology correspond to LG5 (Taboada et al. 2014), supporting the genetic mapping (Figure 2).

Bottom Line: Six genes were located to the SD region, two of them associated with gonad development (sox2 and dnajc19).Genetic diversity and differentiation for 25 LG5 genetic markers showed no differences between males and females sampled from a wild population, suggesting a recent origin of the SD region in turbot.We also analyzed associations with markers of the most relevant sex-related linkage groups in brill (S. rhombus), a closely related species to turbot; the data suggest that an ancient XX/XY system in brill changed to a ZZ/ZW mechanism in turbot.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética, Facultad de Biología (CIBUS), Universidad de Santiago de Compostela, Avda. Lope Gómez de Marzoa, 15782 Santiago de Compostela, Spain.

Show MeSH
Related in: MedlinePlus