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SNPs in genes functional in starch-sugar interconversion associate with natural variation of tuber starch and sugar content of potato (Solanum tuberosum L.).

Schreiber L, Nader-Nieto AC, Schönhals EM, Walkemeier B, Gebhardt C - G3 (Bethesda) (2014)

Bottom Line: Most positive or negative effects of SNPs on tuber-reducing sugar content were reproducible in two different collections of potato cultivars.An allele of the plastidic starch phosphorylase PHO1a associated with increased tuber starch content was cloned as full-length cDNA and characterized.This mutation might cause reduced enzyme activity due to impaired formation of the active dimers, thereby limiting starch breakdown.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Plant Breeding Research, Department of Plant Breeding and Genetics, 50829 Cologne, Germany.

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Box plots showing the effects of the marker genotypic classes of 12 candidate gene alleles on tuber reducing sugar content after 12 wk of cold storage in the SUGAR40 panel (Table 5). Y-axis: Values for reducing sugar content (glucose plus fructose in mg/100 g dry weight). X-axis: Presence (1) or absence (0) of the allele indicated (B, C, F, L) or allele dosage from 0 to 3 (A, D, E, G, H, I, K, M). Genotype classes represented by one cultivar only are not shown.
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fig3: Box plots showing the effects of the marker genotypic classes of 12 candidate gene alleles on tuber reducing sugar content after 12 wk of cold storage in the SUGAR40 panel (Table 5). Y-axis: Values for reducing sugar content (glucose plus fructose in mg/100 g dry weight). X-axis: Presence (1) or absence (0) of the allele indicated (B, C, F, L) or allele dosage from 0 to 3 (A, D, E, G, H, I, K, M). Genotype classes represented by one cultivar only are not shown.

Mentions: Markers derived from genes functional in starch-sugar interconversion, which were associated with chip quality in the CHIPS-ALL population in the present study (Table 2) as well as in previous studies (Draffehn et al. 2010; Li et al. 2005; 2008; 2013), were genotyped in the SUGAR40 panel, either by allele-specific assays (Stp23-8b = PHO1a-HA, StpL(PHO1b)-3b, StpL(PHO1b)-3e, Pain1-8c, InvGE-6f) or by amplicon sequencing (PWD, PHO1b, AGPaseS, BMY-3, Inv-8/2, InvCD141). Significant effects on reducing sugar content (P ≤ 0.05) were observed for 23 marker alleles in all genes tested. Except for BMY-3-snp2533, the direction of the MFA effect was the same as in the CHIPS-ALL population (Table 5). Boxplots of the 12 most effective marker alleles show the effects of the allele’s presence/absence or increasing dosage on the tuber reducing sugar content after 12 weeks’ cold storage (Figure 3).


SNPs in genes functional in starch-sugar interconversion associate with natural variation of tuber starch and sugar content of potato (Solanum tuberosum L.).

Schreiber L, Nader-Nieto AC, Schönhals EM, Walkemeier B, Gebhardt C - G3 (Bethesda) (2014)

Box plots showing the effects of the marker genotypic classes of 12 candidate gene alleles on tuber reducing sugar content after 12 wk of cold storage in the SUGAR40 panel (Table 5). Y-axis: Values for reducing sugar content (glucose plus fructose in mg/100 g dry weight). X-axis: Presence (1) or absence (0) of the allele indicated (B, C, F, L) or allele dosage from 0 to 3 (A, D, E, G, H, I, K, M). Genotype classes represented by one cultivar only are not shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199688&req=5

fig3: Box plots showing the effects of the marker genotypic classes of 12 candidate gene alleles on tuber reducing sugar content after 12 wk of cold storage in the SUGAR40 panel (Table 5). Y-axis: Values for reducing sugar content (glucose plus fructose in mg/100 g dry weight). X-axis: Presence (1) or absence (0) of the allele indicated (B, C, F, L) or allele dosage from 0 to 3 (A, D, E, G, H, I, K, M). Genotype classes represented by one cultivar only are not shown.
Mentions: Markers derived from genes functional in starch-sugar interconversion, which were associated with chip quality in the CHIPS-ALL population in the present study (Table 2) as well as in previous studies (Draffehn et al. 2010; Li et al. 2005; 2008; 2013), were genotyped in the SUGAR40 panel, either by allele-specific assays (Stp23-8b = PHO1a-HA, StpL(PHO1b)-3b, StpL(PHO1b)-3e, Pain1-8c, InvGE-6f) or by amplicon sequencing (PWD, PHO1b, AGPaseS, BMY-3, Inv-8/2, InvCD141). Significant effects on reducing sugar content (P ≤ 0.05) were observed for 23 marker alleles in all genes tested. Except for BMY-3-snp2533, the direction of the MFA effect was the same as in the CHIPS-ALL population (Table 5). Boxplots of the 12 most effective marker alleles show the effects of the allele’s presence/absence or increasing dosage on the tuber reducing sugar content after 12 weeks’ cold storage (Figure 3).

Bottom Line: Most positive or negative effects of SNPs on tuber-reducing sugar content were reproducible in two different collections of potato cultivars.An allele of the plastidic starch phosphorylase PHO1a associated with increased tuber starch content was cloned as full-length cDNA and characterized.This mutation might cause reduced enzyme activity due to impaired formation of the active dimers, thereby limiting starch breakdown.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Plant Breeding Research, Department of Plant Breeding and Genetics, 50829 Cologne, Germany.

Show MeSH
Related in: MedlinePlus