SNPs in genes functional in starch-sugar interconversion associate with natural variation of tuber starch and sugar content of potato (Solanum tuberosum L.).
Bottom Line: Most positive or negative effects of SNPs on tuber-reducing sugar content were reproducible in two different collections of potato cultivars.An allele of the plastidic starch phosphorylase PHO1a associated with increased tuber starch content was cloned as full-length cDNA and characterized.This mutation might cause reduced enzyme activity due to impaired formation of the active dimers, thereby limiting starch breakdown.
Affiliation: Max Planck Institute for Plant Breeding Research, Department of Plant Breeding and Genetics, 50829 Cologne, Germany.Show MeSH
Related in: MedlinePlus
Mentions: Markers derived from genes functional in starch-sugar interconversion, which were associated with chip quality in the CHIPS-ALL population in the present study (Table 2) as well as in previous studies (Draffehn et al. 2010; Li et al. 2005; 2008; 2013), were genotyped in the SUGAR40 panel, either by allele-specific assays (Stp23-8b = PHO1a-HA, StpL(PHO1b)-3b, StpL(PHO1b)-3e, Pain1-8c, InvGE-6f) or by amplicon sequencing (PWD, PHO1b, AGPaseS, BMY-3, Inv-8/2, InvCD141). Significant effects on reducing sugar content (P ≤ 0.05) were observed for 23 marker alleles in all genes tested. Except for BMY-3-snp2533, the direction of the MFA effect was the same as in the CHIPS-ALL population (Table 5). Boxplots of the 12 most effective marker alleles show the effects of the allele’s presence/absence or increasing dosage on the tuber reducing sugar content after 12 weeks’ cold storage (Figure 3).
Affiliation: Max Planck Institute for Plant Breeding Research, Department of Plant Breeding and Genetics, 50829 Cologne, Germany.