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White cells facilitate opposite- and same-sex mating of opaque cells in Candida albicans.

Tao L, Cao C, Liang W, Guan G, Zhang Q, Nobile CJ, Huang G - PLoS Genet. (2014)

Bottom Line: Modes of sexual reproduction in eukaryotic organisms are extremely diverse.Deletion of genes encoding the pheromone precursor proteins and inactivation of the pheromone response signaling pathway (Ste2-MAPK-Cph1) impair the promoting role of white cells (MTLa) in the sexual mating of opaque cells.This coordination between the two different cell types may be a trade-off strategy between sexual and asexual lifestyles in C. albicans.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

ABSTRACT
Modes of sexual reproduction in eukaryotic organisms are extremely diverse. The human fungal pathogen Candida albicans undergoes a phenotypic switch from the white to the opaque phase in order to become mating-competent. In this study, we report that functionally- and morphologically-differentiated white and opaque cells show a coordinated behavior during mating. Although white cells are mating-incompetent, they can produce sexual pheromones when treated with pheromones of the opposite mating type or by physically interacting with opaque cells of the opposite mating type. In a co-culture system, pheromones released by white cells induce opaque cells to form mating projections, and facilitate both opposite- and same-sex mating of opaque cells. Deletion of genes encoding the pheromone precursor proteins and inactivation of the pheromone response signaling pathway (Ste2-MAPK-Cph1) impair the promoting role of white cells (MTLa) in the sexual mating of opaque cells. White and opaque cells communicate via a paracrine pheromone signaling system, creating an environment conducive to sexual mating. This coordination between the two different cell types may be a trade-off strategy between sexual and asexual lifestyles in C. albicans.

No MeSH data available.


Related in: MedlinePlus

White a cells induce mating projection formation in opaque α cells in a mouse skin infection model.1×107 opaque α cells (GH1349) and 1×107 white “helper” cells were mixed and spotted onto the skin of newborn mice for infection. The wor1Δ/Δ mutant (SZ306w-a), WT “a” (P37005 and SZ306a) and “a/α” strains (SZ306) were used for induction of mating projections. Opaque α cells (GH1349) alone (no white “helper” control) was used as a control. Representative SEM images are shown. W, white cells; O, opaque cells; P, mating projections. Scale bar, 10 µm.
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pgen-1004737-g005: White a cells induce mating projection formation in opaque α cells in a mouse skin infection model.1×107 opaque α cells (GH1349) and 1×107 white “helper” cells were mixed and spotted onto the skin of newborn mice for infection. The wor1Δ/Δ mutant (SZ306w-a), WT “a” (P37005 and SZ306a) and “a/α” strains (SZ306) were used for induction of mating projections. Opaque α cells (GH1349) alone (no white “helper” control) was used as a control. Representative SEM images are shown. W, white cells; O, opaque cells; P, mating projections. Scale bar, 10 µm.

Mentions: To evaluate the in vivo relevance of our findings, we next tested whether white cells could facilitate opaque cell mating in a mammalian host. As shown in Figure 5, white a cells of the WT strains or the wor1Δ/Δ mutant strain induced the development of mating projections in opaque α cells. However, in the absence of white a cells or in the presence of white a/α cells, opaque α cells were unable to develop mating projections on the mouse skin. Quantitative mating assays also demonstrated that white a cells promoted opaque cell mating in this mouse skin infection model (Table S2). These results demonstrate that white cells are capable of facilitating opaque cell mating in a natural environmental niche.


White cells facilitate opposite- and same-sex mating of opaque cells in Candida albicans.

Tao L, Cao C, Liang W, Guan G, Zhang Q, Nobile CJ, Huang G - PLoS Genet. (2014)

White a cells induce mating projection formation in opaque α cells in a mouse skin infection model.1×107 opaque α cells (GH1349) and 1×107 white “helper” cells were mixed and spotted onto the skin of newborn mice for infection. The wor1Δ/Δ mutant (SZ306w-a), WT “a” (P37005 and SZ306a) and “a/α” strains (SZ306) were used for induction of mating projections. Opaque α cells (GH1349) alone (no white “helper” control) was used as a control. Representative SEM images are shown. W, white cells; O, opaque cells; P, mating projections. Scale bar, 10 µm.
© Copyright Policy
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4199524&req=5

pgen-1004737-g005: White a cells induce mating projection formation in opaque α cells in a mouse skin infection model.1×107 opaque α cells (GH1349) and 1×107 white “helper” cells were mixed and spotted onto the skin of newborn mice for infection. The wor1Δ/Δ mutant (SZ306w-a), WT “a” (P37005 and SZ306a) and “a/α” strains (SZ306) were used for induction of mating projections. Opaque α cells (GH1349) alone (no white “helper” control) was used as a control. Representative SEM images are shown. W, white cells; O, opaque cells; P, mating projections. Scale bar, 10 µm.
Mentions: To evaluate the in vivo relevance of our findings, we next tested whether white cells could facilitate opaque cell mating in a mammalian host. As shown in Figure 5, white a cells of the WT strains or the wor1Δ/Δ mutant strain induced the development of mating projections in opaque α cells. However, in the absence of white a cells or in the presence of white a/α cells, opaque α cells were unable to develop mating projections on the mouse skin. Quantitative mating assays also demonstrated that white a cells promoted opaque cell mating in this mouse skin infection model (Table S2). These results demonstrate that white cells are capable of facilitating opaque cell mating in a natural environmental niche.

Bottom Line: Modes of sexual reproduction in eukaryotic organisms are extremely diverse.Deletion of genes encoding the pheromone precursor proteins and inactivation of the pheromone response signaling pathway (Ste2-MAPK-Cph1) impair the promoting role of white cells (MTLa) in the sexual mating of opaque cells.This coordination between the two different cell types may be a trade-off strategy between sexual and asexual lifestyles in C. albicans.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

ABSTRACT
Modes of sexual reproduction in eukaryotic organisms are extremely diverse. The human fungal pathogen Candida albicans undergoes a phenotypic switch from the white to the opaque phase in order to become mating-competent. In this study, we report that functionally- and morphologically-differentiated white and opaque cells show a coordinated behavior during mating. Although white cells are mating-incompetent, they can produce sexual pheromones when treated with pheromones of the opposite mating type or by physically interacting with opaque cells of the opposite mating type. In a co-culture system, pheromones released by white cells induce opaque cells to form mating projections, and facilitate both opposite- and same-sex mating of opaque cells. Deletion of genes encoding the pheromone precursor proteins and inactivation of the pheromone response signaling pathway (Ste2-MAPK-Cph1) impair the promoting role of white cells (MTLa) in the sexual mating of opaque cells. White and opaque cells communicate via a paracrine pheromone signaling system, creating an environment conducive to sexual mating. This coordination between the two different cell types may be a trade-off strategy between sexual and asexual lifestyles in C. albicans.

No MeSH data available.


Related in: MedlinePlus