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Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside induced lipid peroxidation in rats' pancreas by phenolic extracts of avocado pear leaves and fruit.

Oboh G, Isaac AT, Akinyemi AJ, Ajani RA - Int J Biomed Sci (2014)

Bottom Line: The result revealed that the leaves had fruit of avocado pear inhibit both α-amylase and α-glucosidase activities in a dose dependent manner.Furthermore, incubation of the rat pancreas in the presence of 5 mM SNP caused an increase in the malondialdehyde (MDA) content in the tissue, however, introduction of the phenolic extracts inhibited MDA produced in a dose dependent manner.The additive and/or synergistic action of major phenolic compounds such as syringic acid, eugenol, vnillic acid, isoeugenol, guaiacol, kaemferol, catechin, ρ-hydroxybenzoic acid, ferulic acid, apigenin, naringenin, epigallocatechin, epicatechin, lupeol and epigallocatechin-3-O-gallate in avocado pear using gas chromatography (GC) could have contributed to the observed medicinal properties of the plant.

View Article: PubMed Central - PubMed

Affiliation: Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, Nigeria, P.M.B. 704, Akure, 340001, Nigeria;

ABSTRACT
Persea americana fruit and leaves had been known in folk medicine for their anti-diabetic prowess. Therefore, this study sought to investigate the inhibitory effect of phenolic extract from avocado pear (Persea americana) leaves and fruits on some key enzymes linked to type 2 diabetes (α-amylase and α-glucosidase); and sodium nitroprusside (SNP) induced lipid peroxidation in rats' pancreas in vitro. The phenolic extracts of Persea americana fruit and leaves were extracted using methanol and 1M HCl (1:1 v/v). Thereafter, their inhibitory effects on sodium nitroprusside induced lipid peroxidation and key enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) were determined in vitro. The result revealed that the leaves had fruit of avocado pear inhibit both α-amylase and α-glucosidase activities in a dose dependent manner. However, the Peel had the highest α-amylase inhibitory activity while the leaf had the highest α-glucosidase inhibitory activity as revealed by their IC50 value. Furthermore, incubation of the rat pancreas in the presence of 5 mM SNP caused an increase in the malondialdehyde (MDA) content in the tissue, however, introduction of the phenolic extracts inhibited MDA produced in a dose dependent manner. The additive and/or synergistic action of major phenolic compounds such as syringic acid, eugenol, vnillic acid, isoeugenol, guaiacol, kaemferol, catechin, ρ-hydroxybenzoic acid, ferulic acid, apigenin, naringenin, epigallocatechin, epicatechin, lupeol and epigallocatechin-3-O-gallate in avocado pear using gas chromatography (GC) could have contributed to the observed medicinal properties of the plant. Therefore, inhibition of some key enzymes linked to type 2 diabetes and prevention of oxidative stress in the pancreas could be some of the possible mechanism by which they exert their anti-diabetic properties.

No MeSH data available.


Related in: MedlinePlus

α-Amylase inhibition activity of Persea americana leaves and fruit parts extract.
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Figure 1: α-Amylase inhibition activity of Persea americana leaves and fruit parts extract.

Mentions: The inhibitory effect of Avocado (Persea americana) pear leaves and fruit parts on both α-amylase and α-glucosidase are presented in Fig. 1 and Fig. 2 respectively. The IC50 (extract concentration that will inhibit 50% enzyme activity) was also estimated as shown in Table 1 below. The result revealed that all the extracts inhibited α-amylase in a dose-dependent manner (in the range of 0–0.164 mg/mL), however, as revealed by the IC50 values (Table 1), phenolic extract from the peel of avocado pear (IC50=0.057 mg/mL) had the highest inhibitory effect on α-amylase activity while the leaf (IC50=0.219 mg/mL) had the least. Also, the ability of the phenolic extracts from avocado pear (Persea americana) fruit parts and leaves to inhibit α-glucosidase activity in vitro was investigated and the result is presented in Figure 2. All the extracts exhibited a dose-dependent enzyme inhibitory activity in the range of 0–0.164 mg/mL, however, the leaf (IC50=0.067 mg/mL) exhibited the highest inhibitory effect while the peel (IC50=0.241 mg/mL) had the least when taking into account the IC50 values of the phenolic extracts (Table 1).


Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside induced lipid peroxidation in rats' pancreas by phenolic extracts of avocado pear leaves and fruit.

Oboh G, Isaac AT, Akinyemi AJ, Ajani RA - Int J Biomed Sci (2014)

α-Amylase inhibition activity of Persea americana leaves and fruit parts extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199475&req=5

Figure 1: α-Amylase inhibition activity of Persea americana leaves and fruit parts extract.
Mentions: The inhibitory effect of Avocado (Persea americana) pear leaves and fruit parts on both α-amylase and α-glucosidase are presented in Fig. 1 and Fig. 2 respectively. The IC50 (extract concentration that will inhibit 50% enzyme activity) was also estimated as shown in Table 1 below. The result revealed that all the extracts inhibited α-amylase in a dose-dependent manner (in the range of 0–0.164 mg/mL), however, as revealed by the IC50 values (Table 1), phenolic extract from the peel of avocado pear (IC50=0.057 mg/mL) had the highest inhibitory effect on α-amylase activity while the leaf (IC50=0.219 mg/mL) had the least. Also, the ability of the phenolic extracts from avocado pear (Persea americana) fruit parts and leaves to inhibit α-glucosidase activity in vitro was investigated and the result is presented in Figure 2. All the extracts exhibited a dose-dependent enzyme inhibitory activity in the range of 0–0.164 mg/mL, however, the leaf (IC50=0.067 mg/mL) exhibited the highest inhibitory effect while the peel (IC50=0.241 mg/mL) had the least when taking into account the IC50 values of the phenolic extracts (Table 1).

Bottom Line: The result revealed that the leaves had fruit of avocado pear inhibit both α-amylase and α-glucosidase activities in a dose dependent manner.Furthermore, incubation of the rat pancreas in the presence of 5 mM SNP caused an increase in the malondialdehyde (MDA) content in the tissue, however, introduction of the phenolic extracts inhibited MDA produced in a dose dependent manner.The additive and/or synergistic action of major phenolic compounds such as syringic acid, eugenol, vnillic acid, isoeugenol, guaiacol, kaemferol, catechin, ρ-hydroxybenzoic acid, ferulic acid, apigenin, naringenin, epigallocatechin, epicatechin, lupeol and epigallocatechin-3-O-gallate in avocado pear using gas chromatography (GC) could have contributed to the observed medicinal properties of the plant.

View Article: PubMed Central - PubMed

Affiliation: Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, Nigeria, P.M.B. 704, Akure, 340001, Nigeria;

ABSTRACT
Persea americana fruit and leaves had been known in folk medicine for their anti-diabetic prowess. Therefore, this study sought to investigate the inhibitory effect of phenolic extract from avocado pear (Persea americana) leaves and fruits on some key enzymes linked to type 2 diabetes (α-amylase and α-glucosidase); and sodium nitroprusside (SNP) induced lipid peroxidation in rats' pancreas in vitro. The phenolic extracts of Persea americana fruit and leaves were extracted using methanol and 1M HCl (1:1 v/v). Thereafter, their inhibitory effects on sodium nitroprusside induced lipid peroxidation and key enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) were determined in vitro. The result revealed that the leaves had fruit of avocado pear inhibit both α-amylase and α-glucosidase activities in a dose dependent manner. However, the Peel had the highest α-amylase inhibitory activity while the leaf had the highest α-glucosidase inhibitory activity as revealed by their IC50 value. Furthermore, incubation of the rat pancreas in the presence of 5 mM SNP caused an increase in the malondialdehyde (MDA) content in the tissue, however, introduction of the phenolic extracts inhibited MDA produced in a dose dependent manner. The additive and/or synergistic action of major phenolic compounds such as syringic acid, eugenol, vnillic acid, isoeugenol, guaiacol, kaemferol, catechin, ρ-hydroxybenzoic acid, ferulic acid, apigenin, naringenin, epigallocatechin, epicatechin, lupeol and epigallocatechin-3-O-gallate in avocado pear using gas chromatography (GC) could have contributed to the observed medicinal properties of the plant. Therefore, inhibition of some key enzymes linked to type 2 diabetes and prevention of oxidative stress in the pancreas could be some of the possible mechanism by which they exert their anti-diabetic properties.

No MeSH data available.


Related in: MedlinePlus