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Assessment of Pharmacokinetics and Toxicology of Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) in Rats and Goats.

Al-Habdan I, Sadat-Ali M, Safar Alghamdy M, Randhawa A, Chathoth S - Int J Biomed Sci (2014)

Bottom Line: The gross observations of the various organs appeared normal and histopathological studies did not show any toxicity in the organs tested.  The plasma concentration-time profile of SHMSP after intramuscular injection, the level of SHMSP in an initial high phase reaching the highest at 30 minutes 2.3184 ng/ml and 60 minutes 1.7447 ng/ml at 60 minutes.The lowest level was at 360 minutes of 0.0879 ng/ml.The dose of SHMSP at 20 times the recommended dose was not toxic and secondly the peak time and level was at 30 minutes to 120 minutes and the plasma half-life of SHMSP was 90 minutes.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, University of Dammam, Saudi Arabia;

ABSTRACT

Background: Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) was discovered and patented with USPTO in 2008. Studies have shown that SHMSP works as an angiogenesis factor. This study was done to evaluate pharmacokinetics (PK) in rats and toxicology studies in goats and rats.

Methods: In 80 skeletally mature Sprague Dawley rats 5 milligram/kg body weight of SHMSP was injected intramuscularly. Blood samples were collected at 0, 30, 60, 120, 240, 320 minutes and 480 minutes. The plasma calibration curves were prepared at concentrations of 6.25, 3.12, 1.56, 0.78 and 0.39 ng/mL by spiking 190 µL of rat plasma with 10µL of working standard and 200 µL of deionized water. Samples were vortexed for five seconds, centrifuged at 14000 rpm for 30 minutes at 4°C and the supernatant was collected analyzed using High-performance liquid chromatography (HPLC). After injection of 20 µL sample, the peptide was eluted with 15ml linear gradient up to 36% of eluent A. The time between injections was 25 min. and the eluent was monitored at a wavelength of 215 nm. The concentration of peptide present in the rat plasma samples collected at different time intervals were quantified using standard curve method. The goats were injected deep intramuscularly 100 mg/kg-body weight of the SHMSP dissolved in injection solution. In 10 Sprague Dawley rats of ≥250 grams of weight, 20 mg/kg/day SHMSP was injected for 7 consecutive days. All the animals were kept at a close watch. Clinical observation at least once daily and as necessary was undertaken. After 2 weeks animals were euthanized and major organs were harvested and histopathology samples were obtained and processed.

Results: There were no deaths is either of the study and control group of animals. The gross observations of the various organs appeared normal and histopathological studies did not show any toxicity in the organs tested.  The plasma concentration-time profile of SHMSP after intramuscular injection, the level of SHMSP in an initial high phase reaching the highest at 30 minutes 2.3184 ng/ml and 60 minutes 1.7447 ng/ml at 60 minutes. The lowest level was at 360 minutes of 0.0879 ng/ml.

Conclusions: The dose of SHMSP at 20 times the recommended dose was not toxic and secondly the peak time and level was at 30 minutes to 120 minutes and the plasma half-life of SHMSP was 90 minutes.

No MeSH data available.


Related in: MedlinePlus

Standard curve obtained using serially diluted known concentration of the standard peptide.
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Figure 1: Standard curve obtained using serially diluted known concentration of the standard peptide.

Mentions: Acetonitrile (HPLC grade, Fischer Scientific) and Milli-Q water was used for the analysis. Analysis was performed using HPLC Waters 2535 quaternary gradient module system with Waters 2489 UV detector and Waters 2707 auto-sampler. Separations were carried out on a Waters BioSuite C18 analytical column (4.6 × 250 mm) connected to a guard (3.9 × 20 mm) column and the system was operated at an ambient temperature. The column was equilibrated with 76% eluent A (water) and 24% eluent B (acetonitrile) with both containing 0.1% trifluoroacetic acid at a flow rate of 1.0 mL/min as mobile phase. After injection of 20 µL sample, the peptide was eluted with 15ml linear gradient up to 36% of eluent A. The time between injections was 25 min. and the eluent was monitored at a wavelength of 215 nm. Plasma was isolated, processed and 20 μL was injected to HPLC system. The concentrations obtained after three animals injections have been tabulated as Run 1, Run 2 and Run 3 (Table 1). The concentration of peptide present in the rat plasma samples collected at different time intervals were quantified using standard curve method. The unknown concentrations of peptide present in the plasma samples were calculated applying the value of peak area in the equation obtained from the standard curve (Figure 1). Data shown are mean ± SD (n=3).


Assessment of Pharmacokinetics and Toxicology of Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) in Rats and Goats.

Al-Habdan I, Sadat-Ali M, Safar Alghamdy M, Randhawa A, Chathoth S - Int J Biomed Sci (2014)

Standard curve obtained using serially diluted known concentration of the standard peptide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199474&req=5

Figure 1: Standard curve obtained using serially diluted known concentration of the standard peptide.
Mentions: Acetonitrile (HPLC grade, Fischer Scientific) and Milli-Q water was used for the analysis. Analysis was performed using HPLC Waters 2535 quaternary gradient module system with Waters 2489 UV detector and Waters 2707 auto-sampler. Separations were carried out on a Waters BioSuite C18 analytical column (4.6 × 250 mm) connected to a guard (3.9 × 20 mm) column and the system was operated at an ambient temperature. The column was equilibrated with 76% eluent A (water) and 24% eluent B (acetonitrile) with both containing 0.1% trifluoroacetic acid at a flow rate of 1.0 mL/min as mobile phase. After injection of 20 µL sample, the peptide was eluted with 15ml linear gradient up to 36% of eluent A. The time between injections was 25 min. and the eluent was monitored at a wavelength of 215 nm. Plasma was isolated, processed and 20 μL was injected to HPLC system. The concentrations obtained after three animals injections have been tabulated as Run 1, Run 2 and Run 3 (Table 1). The concentration of peptide present in the rat plasma samples collected at different time intervals were quantified using standard curve method. The unknown concentrations of peptide present in the plasma samples were calculated applying the value of peak area in the equation obtained from the standard curve (Figure 1). Data shown are mean ± SD (n=3).

Bottom Line: The gross observations of the various organs appeared normal and histopathological studies did not show any toxicity in the organs tested.  The plasma concentration-time profile of SHMSP after intramuscular injection, the level of SHMSP in an initial high phase reaching the highest at 30 minutes 2.3184 ng/ml and 60 minutes 1.7447 ng/ml at 60 minutes.The lowest level was at 360 minutes of 0.0879 ng/ml.The dose of SHMSP at 20 times the recommended dose was not toxic and secondly the peak time and level was at 30 minutes to 120 minutes and the plasma half-life of SHMSP was 90 minutes.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, University of Dammam, Saudi Arabia;

ABSTRACT

Background: Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) was discovered and patented with USPTO in 2008. Studies have shown that SHMSP works as an angiogenesis factor. This study was done to evaluate pharmacokinetics (PK) in rats and toxicology studies in goats and rats.

Methods: In 80 skeletally mature Sprague Dawley rats 5 milligram/kg body weight of SHMSP was injected intramuscularly. Blood samples were collected at 0, 30, 60, 120, 240, 320 minutes and 480 minutes. The plasma calibration curves were prepared at concentrations of 6.25, 3.12, 1.56, 0.78 and 0.39 ng/mL by spiking 190 µL of rat plasma with 10µL of working standard and 200 µL of deionized water. Samples were vortexed for five seconds, centrifuged at 14000 rpm for 30 minutes at 4°C and the supernatant was collected analyzed using High-performance liquid chromatography (HPLC). After injection of 20 µL sample, the peptide was eluted with 15ml linear gradient up to 36% of eluent A. The time between injections was 25 min. and the eluent was monitored at a wavelength of 215 nm. The concentration of peptide present in the rat plasma samples collected at different time intervals were quantified using standard curve method. The goats were injected deep intramuscularly 100 mg/kg-body weight of the SHMSP dissolved in injection solution. In 10 Sprague Dawley rats of ≥250 grams of weight, 20 mg/kg/day SHMSP was injected for 7 consecutive days. All the animals were kept at a close watch. Clinical observation at least once daily and as necessary was undertaken. After 2 weeks animals were euthanized and major organs were harvested and histopathology samples were obtained and processed.

Results: There were no deaths is either of the study and control group of animals. The gross observations of the various organs appeared normal and histopathological studies did not show any toxicity in the organs tested.  The plasma concentration-time profile of SHMSP after intramuscular injection, the level of SHMSP in an initial high phase reaching the highest at 30 minutes 2.3184 ng/ml and 60 minutes 1.7447 ng/ml at 60 minutes. The lowest level was at 360 minutes of 0.0879 ng/ml.

Conclusions: The dose of SHMSP at 20 times the recommended dose was not toxic and secondly the peak time and level was at 30 minutes to 120 minutes and the plasma half-life of SHMSP was 90 minutes.

No MeSH data available.


Related in: MedlinePlus