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Taurine supplementation reduces oxidative stress and protects the liver in an iron-overload murine model.

Zhang Z, Liu D, Yi B, Liao Z, Tang L, Yin D, He M - Mol Med Rep (2014)

Bottom Line: Taurine supplementation increased liver taurine levels by 40% and led to improved liver function, as well as a reduction in apoptosis, ROS formation and mitochondrial swelling and an attenuation in the loss of the mitochondrial membrane potential.These results indicate that taurine reduces iron‑induced hepatic oxidative stress, preserves liver function and inhibits hepatocyte apoptosis.Therefore, taurine may be a potential therapeutic drug to reduce liver damage caused by iron overload.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, P.R. China.

ABSTRACT
We previously demonstrated that iron overload induces liver damage by causing the formation of reactive oxygen species (ROS). Taurine is a potent free radical scavenger that attenuates the damage caused by excessive oxygen free radicals. Therefore, the aim of the present study was to investigate whether taurine could reduce the hepatotoxicity of iron overload with regard to ROS production. Mice were intraperitoneally injected with iron 5 days/week for 13 weeks to achieve iron overload. It was found that iron overload resulted in liver dysfunction, increased apoptosis and elevated oxidative stress. Taurine supplementation increased liver taurine levels by 40% and led to improved liver function, as well as a reduction in apoptosis, ROS formation and mitochondrial swelling and an attenuation in the loss of the mitochondrial membrane potential. Treatment with taurine mediated a reduction in oxidative stress in iron‑overloaded mice, attenuated liver lipid peroxidation, elevated antioxidant enzyme activities and maintained reduced glutathione levels. These results indicate that taurine reduces iron‑induced hepatic oxidative stress, preserves liver function and inhibits hepatocyte apoptosis. Therefore, taurine may be a potential therapeutic drug to reduce liver damage caused by iron overload.

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Effects of taurine on hepatic intercellular reactive oxygen species generation in mice injected with iron over a 13-week period. Data are presented as the mean ± standard error of the mean (n=12). aP>0.05 vs. the placebo + vehicle group; bP<0.01 vs. the placebo + vehicle and placebo + taurine groups and cP<0.01 vs. the iron + vehicle group. DCF, dichloro-dihydro-fluorescein.
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f4-mmr-10-05-2255: Effects of taurine on hepatic intercellular reactive oxygen species generation in mice injected with iron over a 13-week period. Data are presented as the mean ± standard error of the mean (n=12). aP>0.05 vs. the placebo + vehicle group; bP<0.01 vs. the placebo + vehicle and placebo + taurine groups and cP<0.01 vs. the iron + vehicle group. DCF, dichloro-dihydro-fluorescein.

Mentions: It is well established that iron induces toxicity in the liver, as iron is primarily stored in the liver and produces ROS (33). Therefore, the intracellular ROS levels were measured using a DCFH-DA assay. The ROS levels were observed to be significantly increased in the iron-treated animals compared with those in the untreated animals (P<0.01). This indicates that supplementation with taurine significantly prevents ROS formation induced by iron overload (Fig. 4).


Taurine supplementation reduces oxidative stress and protects the liver in an iron-overload murine model.

Zhang Z, Liu D, Yi B, Liao Z, Tang L, Yin D, He M - Mol Med Rep (2014)

Effects of taurine on hepatic intercellular reactive oxygen species generation in mice injected with iron over a 13-week period. Data are presented as the mean ± standard error of the mean (n=12). aP>0.05 vs. the placebo + vehicle group; bP<0.01 vs. the placebo + vehicle and placebo + taurine groups and cP<0.01 vs. the iron + vehicle group. DCF, dichloro-dihydro-fluorescein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199407&req=5

f4-mmr-10-05-2255: Effects of taurine on hepatic intercellular reactive oxygen species generation in mice injected with iron over a 13-week period. Data are presented as the mean ± standard error of the mean (n=12). aP>0.05 vs. the placebo + vehicle group; bP<0.01 vs. the placebo + vehicle and placebo + taurine groups and cP<0.01 vs. the iron + vehicle group. DCF, dichloro-dihydro-fluorescein.
Mentions: It is well established that iron induces toxicity in the liver, as iron is primarily stored in the liver and produces ROS (33). Therefore, the intracellular ROS levels were measured using a DCFH-DA assay. The ROS levels were observed to be significantly increased in the iron-treated animals compared with those in the untreated animals (P<0.01). This indicates that supplementation with taurine significantly prevents ROS formation induced by iron overload (Fig. 4).

Bottom Line: Taurine supplementation increased liver taurine levels by 40% and led to improved liver function, as well as a reduction in apoptosis, ROS formation and mitochondrial swelling and an attenuation in the loss of the mitochondrial membrane potential.These results indicate that taurine reduces iron‑induced hepatic oxidative stress, preserves liver function and inhibits hepatocyte apoptosis.Therefore, taurine may be a potential therapeutic drug to reduce liver damage caused by iron overload.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, P.R. China.

ABSTRACT
We previously demonstrated that iron overload induces liver damage by causing the formation of reactive oxygen species (ROS). Taurine is a potent free radical scavenger that attenuates the damage caused by excessive oxygen free radicals. Therefore, the aim of the present study was to investigate whether taurine could reduce the hepatotoxicity of iron overload with regard to ROS production. Mice were intraperitoneally injected with iron 5 days/week for 13 weeks to achieve iron overload. It was found that iron overload resulted in liver dysfunction, increased apoptosis and elevated oxidative stress. Taurine supplementation increased liver taurine levels by 40% and led to improved liver function, as well as a reduction in apoptosis, ROS formation and mitochondrial swelling and an attenuation in the loss of the mitochondrial membrane potential. Treatment with taurine mediated a reduction in oxidative stress in iron‑overloaded mice, attenuated liver lipid peroxidation, elevated antioxidant enzyme activities and maintained reduced glutathione levels. These results indicate that taurine reduces iron‑induced hepatic oxidative stress, preserves liver function and inhibits hepatocyte apoptosis. Therefore, taurine may be a potential therapeutic drug to reduce liver damage caused by iron overload.

Show MeSH
Related in: MedlinePlus