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Localized micro- and nano-scale remodelling in the diabetic aorta.

Akhtar R, Cruickshank JK, Zhao X, Walton LA, Gardiner NJ, Barrett SD, Graham HK, Derby B, Sherratt MJ - Acta Biomater (2014)

Bottom Line: We found that the medial aortic layer underwent significant thickening in diabetic animals but without significant changes in collagen or elastin (abundance).Scanning acoustic microscopy demonstrated that such tissue remodelling was associated with a significant decrease in acoustic wave speed (an indicator of reduced material stiffness) in the inter-lamellar spaces of the vessel wall.This index of decreased stiffness was also linked to increased extracellular protease activity (assessed by semi-quantitative in situ gelatin zymography).

View Article: PubMed Central - PubMed

Affiliation: Centre for Materials and Structures, School of Engineering, University of Liverpool, Liverpool L69 3GH, UK. Electronic address: r.akhtar@liverpool.ac.uk.

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(a) SAM images of rat aorta (200 × 200 μm) prior to any fixation or staining. Lamellar (L) and inter-lamellar (IL) regions of the aorta are highlighted. (b) The same section as shown in (a) following polarized light microscopy of Picro-sirius red (PSR) stained and hence birefringent collagen fibres. Scale bar 50 μm.
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f0005: (a) SAM images of rat aorta (200 × 200 μm) prior to any fixation or staining. Lamellar (L) and inter-lamellar (IL) regions of the aorta are highlighted. (b) The same section as shown in (a) following polarized light microscopy of Picro-sirius red (PSR) stained and hence birefringent collagen fibres. Scale bar 50 μm.

Mentions: The resulting SAM images contain sufficient structural information to allow the acoustic wave speed of the elastic lamellae and inter-lamellar regions of the aortic wall to be measured independently (Fig. 1).


Localized micro- and nano-scale remodelling in the diabetic aorta.

Akhtar R, Cruickshank JK, Zhao X, Walton LA, Gardiner NJ, Barrett SD, Graham HK, Derby B, Sherratt MJ - Acta Biomater (2014)

(a) SAM images of rat aorta (200 × 200 μm) prior to any fixation or staining. Lamellar (L) and inter-lamellar (IL) regions of the aorta are highlighted. (b) The same section as shown in (a) following polarized light microscopy of Picro-sirius red (PSR) stained and hence birefringent collagen fibres. Scale bar 50 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4199142&req=5

f0005: (a) SAM images of rat aorta (200 × 200 μm) prior to any fixation or staining. Lamellar (L) and inter-lamellar (IL) regions of the aorta are highlighted. (b) The same section as shown in (a) following polarized light microscopy of Picro-sirius red (PSR) stained and hence birefringent collagen fibres. Scale bar 50 μm.
Mentions: The resulting SAM images contain sufficient structural information to allow the acoustic wave speed of the elastic lamellae and inter-lamellar regions of the aortic wall to be measured independently (Fig. 1).

Bottom Line: We found that the medial aortic layer underwent significant thickening in diabetic animals but without significant changes in collagen or elastin (abundance).Scanning acoustic microscopy demonstrated that such tissue remodelling was associated with a significant decrease in acoustic wave speed (an indicator of reduced material stiffness) in the inter-lamellar spaces of the vessel wall.This index of decreased stiffness was also linked to increased extracellular protease activity (assessed by semi-quantitative in situ gelatin zymography).

View Article: PubMed Central - PubMed

Affiliation: Centre for Materials and Structures, School of Engineering, University of Liverpool, Liverpool L69 3GH, UK. Electronic address: r.akhtar@liverpool.ac.uk.

Show MeSH
Related in: MedlinePlus