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Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy.

McMullan G, Faruqi AR, Clare D, Henderson R - Ultramicroscopy (2014)

Bottom Line: Of these, the DQE is the most important in the study of radiation sensitive samples where detector performance is crucial.We find that all three detectors have a better DQE than film.The K2 Summit has the best DQE at low spatial frequencies but with increasing spatial frequency its DQE falls below that of the Falcon II.

View Article: PubMed Central - PubMed

Affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom. Electronic address: gm2@mrc-lmb.cam.ac.uk.

No MeSH data available.


Related in: MedlinePlus

Measured MTF as a function of spatial frequency. The solid lines are for the DE-20 (green), Falcon II (red), and K2 Summit in super-resolution mode (blue). The dotted blue line is the corresponding K2 Summit result in normal resolution mode. The dotted red line is the MTF obtained for the Falcon II via the noise power spectra method.
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f0010: Measured MTF as a function of spatial frequency. The solid lines are for the DE-20 (green), Falcon II (red), and K2 Summit in super-resolution mode (blue). The dotted blue line is the corresponding K2 Summit result in normal resolution mode. The dotted red line is the MTF obtained for the Falcon II via the noise power spectra method.

Mentions: The measured MTF as a function of spatial frequency for the three detectors is shown in Fig. 2. The corresponding edge spread functions and Gaussian expansion fits are given as supplementary data. The MTF of the K2 Summit was obtained both in normal and super-resolution (but plotted only out to the physical Nyquist frequency). Using super-resolution leads to a significant improvement in the MTF but the actual enhancement is less than that expected from the reduction in the pixel modulation factor, i.e., that would be expected if the hardware centroiding algorithm used by the K2 worked perfectly. The results for the Falcon II MTF calculated using both the edge and noise power spectra methods agree very well. While the MTF of the Falcon II is lowest, the agreement between these two methods for calculating the MTF indicates that to a first approximation its response to incident electrons can be described by a simple point spread function.


Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy.

McMullan G, Faruqi AR, Clare D, Henderson R - Ultramicroscopy (2014)

Measured MTF as a function of spatial frequency. The solid lines are for the DE-20 (green), Falcon II (red), and K2 Summit in super-resolution mode (blue). The dotted blue line is the corresponding K2 Summit result in normal resolution mode. The dotted red line is the MTF obtained for the Falcon II via the noise power spectra method.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4199116&req=5

f0010: Measured MTF as a function of spatial frequency. The solid lines are for the DE-20 (green), Falcon II (red), and K2 Summit in super-resolution mode (blue). The dotted blue line is the corresponding K2 Summit result in normal resolution mode. The dotted red line is the MTF obtained for the Falcon II via the noise power spectra method.
Mentions: The measured MTF as a function of spatial frequency for the three detectors is shown in Fig. 2. The corresponding edge spread functions and Gaussian expansion fits are given as supplementary data. The MTF of the K2 Summit was obtained both in normal and super-resolution (but plotted only out to the physical Nyquist frequency). Using super-resolution leads to a significant improvement in the MTF but the actual enhancement is less than that expected from the reduction in the pixel modulation factor, i.e., that would be expected if the hardware centroiding algorithm used by the K2 worked perfectly. The results for the Falcon II MTF calculated using both the edge and noise power spectra methods agree very well. While the MTF of the Falcon II is lowest, the agreement between these two methods for calculating the MTF indicates that to a first approximation its response to incident electrons can be described by a simple point spread function.

Bottom Line: Of these, the DQE is the most important in the study of radiation sensitive samples where detector performance is crucial.We find that all three detectors have a better DQE than film.The K2 Summit has the best DQE at low spatial frequencies but with increasing spatial frequency its DQE falls below that of the Falcon II.

View Article: PubMed Central - PubMed

Affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom. Electronic address: gm2@mrc-lmb.cam.ac.uk.

No MeSH data available.


Related in: MedlinePlus