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Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy.

McMullan G, Faruqi AR, Clare D, Henderson R - Ultramicroscopy (2014)

Bottom Line: Of these, the DQE is the most important in the study of radiation sensitive samples where detector performance is crucial.We find that all three detectors have a better DQE than film.The K2 Summit has the best DQE at low spatial frequencies but with increasing spatial frequency its DQE falls below that of the Falcon II.

View Article: PubMed Central - PubMed

Affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom. Electronic address: gm2@mrc-lmb.cam.ac.uk.

No MeSH data available.


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Randomly chosen 256×256 areas from single frames showing individual 300 keV electron events as recorded on the (a) DE-20, (b) Falcon II and (c) K2 Summit detectors. The images are normalised so that the RMS background noise has a value of 1. In (d) the Landau distribution, , is compared with the measured probability distributions for events as a function of integrated event signal, , in the three detectors. The measured distributions have been scaled by the fitted width parameter, ξ, and plotted using  in which  is the position of the maximum of ,  is the position of the most probable value. The measured ratios of  to the RMS background noise for the DE-20, Falcon II and K2 Summit are 49.6, 30.6 and 19.6 respectively, while the corresponding ratios of  to ξ are 4.8, 5.3, and 5.4.
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f0005: Randomly chosen 256×256 areas from single frames showing individual 300 keV electron events as recorded on the (a) DE-20, (b) Falcon II and (c) K2 Summit detectors. The images are normalised so that the RMS background noise has a value of 1. In (d) the Landau distribution, , is compared with the measured probability distributions for events as a function of integrated event signal, , in the three detectors. The measured distributions have been scaled by the fitted width parameter, ξ, and plotted using in which is the position of the maximum of , is the position of the most probable value. The measured ratios of to the RMS background noise for the DE-20, Falcon II and K2 Summit are 49.6, 30.6 and 19.6 respectively, while the corresponding ratios of to ξ are 4.8, 5.3, and 5.4.

Mentions: The physical properties of the three detectors are summarised in Table 1. The high sensitivity of MAPS detectors to individual 300 keV electrons is illustrated in Fig. 1(a–c). These show images of single electron events as recorded in single frames on the detectors. Single frame output is not currently supported on the K2 Summit and in order to obtain this image the data-stream from the camera was intercepted and decoded. The actual frames and the parts of them that are shown were chosen randomly. In order to compare the detectors the signals have been scaled so that the RMS readout noise in each pixel is 1. The intrinsic variability in the events can be clearly seen as well as the presence of the occasional electron track.


Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy.

McMullan G, Faruqi AR, Clare D, Henderson R - Ultramicroscopy (2014)

Randomly chosen 256×256 areas from single frames showing individual 300 keV electron events as recorded on the (a) DE-20, (b) Falcon II and (c) K2 Summit detectors. The images are normalised so that the RMS background noise has a value of 1. In (d) the Landau distribution, , is compared with the measured probability distributions for events as a function of integrated event signal, , in the three detectors. The measured distributions have been scaled by the fitted width parameter, ξ, and plotted using  in which  is the position of the maximum of ,  is the position of the most probable value. The measured ratios of  to the RMS background noise for the DE-20, Falcon II and K2 Summit are 49.6, 30.6 and 19.6 respectively, while the corresponding ratios of  to ξ are 4.8, 5.3, and 5.4.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4199116&req=5

f0005: Randomly chosen 256×256 areas from single frames showing individual 300 keV electron events as recorded on the (a) DE-20, (b) Falcon II and (c) K2 Summit detectors. The images are normalised so that the RMS background noise has a value of 1. In (d) the Landau distribution, , is compared with the measured probability distributions for events as a function of integrated event signal, , in the three detectors. The measured distributions have been scaled by the fitted width parameter, ξ, and plotted using in which is the position of the maximum of , is the position of the most probable value. The measured ratios of to the RMS background noise for the DE-20, Falcon II and K2 Summit are 49.6, 30.6 and 19.6 respectively, while the corresponding ratios of to ξ are 4.8, 5.3, and 5.4.
Mentions: The physical properties of the three detectors are summarised in Table 1. The high sensitivity of MAPS detectors to individual 300 keV electrons is illustrated in Fig. 1(a–c). These show images of single electron events as recorded in single frames on the detectors. Single frame output is not currently supported on the K2 Summit and in order to obtain this image the data-stream from the camera was intercepted and decoded. The actual frames and the parts of them that are shown were chosen randomly. In order to compare the detectors the signals have been scaled so that the RMS readout noise in each pixel is 1. The intrinsic variability in the events can be clearly seen as well as the presence of the occasional electron track.

Bottom Line: Of these, the DQE is the most important in the study of radiation sensitive samples where detector performance is crucial.We find that all three detectors have a better DQE than film.The K2 Summit has the best DQE at low spatial frequencies but with increasing spatial frequency its DQE falls below that of the Falcon II.

View Article: PubMed Central - PubMed

Affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom. Electronic address: gm2@mrc-lmb.cam.ac.uk.

No MeSH data available.


Related in: MedlinePlus