Limits...
Evaluation of the effects of chronic intoxication with inorganic mercury on memory and motor control in rats.

Teixeira FB, Fernandes RM, Farias-Junior PM, Costa NM, Fernandes LM, Santana LN, Silva-Junior AF, Silva MC, Maia CS, Lima RR - Int J Environ Res Public Health (2014)

Bottom Line: Twenty animals were intoxicated for 45 days at a dose of 0.375 mg/kg/day.This study demonstrates that long-term chronic HgCl2 intoxication in rats promotes functional damage.Additionally, HgCl2 accumulated in both the hippocampus and cortex of the brain with a higher affinity for the cortex.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, 66075-900 Belém-Pará, Brazil. teixeira.f.bruno@gmail.com.

ABSTRACT
The aims of this study were to evaluate whether chronic intoxication with mercury chloride (HgCl2), in a low concentration over a long time, can be deposited in the central nervous tissue and to determine if this exposure induces motor and cognitive impairments. Twenty animals were intoxicated for 45 days at a dose of 0.375 mg/kg/day. After this period, the animals underwent a battery of behavioral tests, in a sequence of open field, social recognition, elevated T maze and rotarod tests. They were then sacrificed, their brains collected and the motor cortex and hippocampus dissected for quantification of mercury deposited. This study demonstrates that long-term chronic HgCl2 intoxication in rats promotes functional damage. Exposure to HgCl2 induced anxiety-related responses, short- and long-term memory impairments and motor deficits. Additionally, HgCl2 accumulated in both the hippocampus and cortex of the brain with a higher affinity for the cortex.

Show MeSH

Related in: MedlinePlus

Schematic representation of the experimental design utilized in the present study. (A) Open field; (B) social recognition apparatus; (C) elevated T maze; (D) rotarod; (E) hippocampus on the left and motor cortex on the right; (F) sample to measure mercury.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4199013&req=5

ijerph-11-09171-f001: Schematic representation of the experimental design utilized in the present study. (A) Open field; (B) social recognition apparatus; (C) elevated T maze; (D) rotarod; (E) hippocampus on the left and motor cortex on the right; (F) sample to measure mercury.

Mentions: All analyses were conducted in duplicates of the group tissue samples, and the values obtained ranged from a confidence interval of ±10% (r: 0.9992). The methodology is summarized in Figure 1.


Evaluation of the effects of chronic intoxication with inorganic mercury on memory and motor control in rats.

Teixeira FB, Fernandes RM, Farias-Junior PM, Costa NM, Fernandes LM, Santana LN, Silva-Junior AF, Silva MC, Maia CS, Lima RR - Int J Environ Res Public Health (2014)

Schematic representation of the experimental design utilized in the present study. (A) Open field; (B) social recognition apparatus; (C) elevated T maze; (D) rotarod; (E) hippocampus on the left and motor cortex on the right; (F) sample to measure mercury.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4199013&req=5

ijerph-11-09171-f001: Schematic representation of the experimental design utilized in the present study. (A) Open field; (B) social recognition apparatus; (C) elevated T maze; (D) rotarod; (E) hippocampus on the left and motor cortex on the right; (F) sample to measure mercury.
Mentions: All analyses were conducted in duplicates of the group tissue samples, and the values obtained ranged from a confidence interval of ±10% (r: 0.9992). The methodology is summarized in Figure 1.

Bottom Line: Twenty animals were intoxicated for 45 days at a dose of 0.375 mg/kg/day.This study demonstrates that long-term chronic HgCl2 intoxication in rats promotes functional damage.Additionally, HgCl2 accumulated in both the hippocampus and cortex of the brain with a higher affinity for the cortex.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, 66075-900 Belém-Pará, Brazil. teixeira.f.bruno@gmail.com.

ABSTRACT
The aims of this study were to evaluate whether chronic intoxication with mercury chloride (HgCl2), in a low concentration over a long time, can be deposited in the central nervous tissue and to determine if this exposure induces motor and cognitive impairments. Twenty animals were intoxicated for 45 days at a dose of 0.375 mg/kg/day. After this period, the animals underwent a battery of behavioral tests, in a sequence of open field, social recognition, elevated T maze and rotarod tests. They were then sacrificed, their brains collected and the motor cortex and hippocampus dissected for quantification of mercury deposited. This study demonstrates that long-term chronic HgCl2 intoxication in rats promotes functional damage. Exposure to HgCl2 induced anxiety-related responses, short- and long-term memory impairments and motor deficits. Additionally, HgCl2 accumulated in both the hippocampus and cortex of the brain with a higher affinity for the cortex.

Show MeSH
Related in: MedlinePlus