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Soil microbial functional and fungal diversity as influenced by municipal sewage sludge accumulation.

Frąc M, Oszust K, Lipiec J, Jezierska-Tys S, Nwaichi EO - Int J Environ Res Public Health (2014)

Bottom Line: Biolog EcoPlatesTM were inoculated with a soil suspension, and the Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H) were calculated to interpret the results.Moreover, the genera of fungi isolated from the soil were identified using microscopic methods.Municipal sewage sludge can serve as a habitat for plant pathogens and as a source of pathogen strains for biotechnological applications.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular and Environmental Microbiology, Department of Plant and Soil System, Institute of Agrophysics, Polish Academy of Sciences, Lublin 20-290, Poland. m.frac@ipan.lublin.pl.

ABSTRACT
Safe disposal of municipal sewage sludge is a challenging global environmental concern. The aim of this study was to assess the response of soil microbial functional diversity to the accumulation of municipal sewage sludge during landfill storage. Soil samples of a municipal sewage sludge (SS) and from a sewage sludge landfill that was 3 m from a SS landfill (SS3) were analyzed relative to an undisturbed reference soil. Biolog EcoPlatesTM were inoculated with a soil suspension, and the Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H) were calculated to interpret the results. The fungi isolated from the sewage sludge were identified using comparative rDNA sequencing of the LSU D2 region. The MicroSEQ® ID software was used to assess the raw sequence files, perform sequence matching to the MicroSEQ® ID-validated reference database and create Neighbor-Joining trees. Moreover, the genera of fungi isolated from the soil were identified using microscopic methods. Municipal sewage sludge can serve as a habitat for plant pathogens and as a source of pathogen strains for biotechnological applications.

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Mean values of colony forming units (CFU) of the fungal communities.
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ijerph-11-08891-f007: Mean values of colony forming units (CFU) of the fungal communities.

Mentions: The effects of sewage sludge accumulation on the total number of fungi are presented in Figure 7. Mean values of colony forming units were greater in the soil under sewage sludge accumulation (80.43 × 106 CFU kg−1) relative to in the control (71.20 × 106 CFU kg−1) and the SS3 soil (55.54 × 106 CFU kg−1).


Soil microbial functional and fungal diversity as influenced by municipal sewage sludge accumulation.

Frąc M, Oszust K, Lipiec J, Jezierska-Tys S, Nwaichi EO - Int J Environ Res Public Health (2014)

Mean values of colony forming units (CFU) of the fungal communities.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4198996&req=5

ijerph-11-08891-f007: Mean values of colony forming units (CFU) of the fungal communities.
Mentions: The effects of sewage sludge accumulation on the total number of fungi are presented in Figure 7. Mean values of colony forming units were greater in the soil under sewage sludge accumulation (80.43 × 106 CFU kg−1) relative to in the control (71.20 × 106 CFU kg−1) and the SS3 soil (55.54 × 106 CFU kg−1).

Bottom Line: Biolog EcoPlatesTM were inoculated with a soil suspension, and the Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H) were calculated to interpret the results.Moreover, the genera of fungi isolated from the soil were identified using microscopic methods.Municipal sewage sludge can serve as a habitat for plant pathogens and as a source of pathogen strains for biotechnological applications.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular and Environmental Microbiology, Department of Plant and Soil System, Institute of Agrophysics, Polish Academy of Sciences, Lublin 20-290, Poland. m.frac@ipan.lublin.pl.

ABSTRACT
Safe disposal of municipal sewage sludge is a challenging global environmental concern. The aim of this study was to assess the response of soil microbial functional diversity to the accumulation of municipal sewage sludge during landfill storage. Soil samples of a municipal sewage sludge (SS) and from a sewage sludge landfill that was 3 m from a SS landfill (SS3) were analyzed relative to an undisturbed reference soil. Biolog EcoPlatesTM were inoculated with a soil suspension, and the Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H) were calculated to interpret the results. The fungi isolated from the sewage sludge were identified using comparative rDNA sequencing of the LSU D2 region. The MicroSEQ® ID software was used to assess the raw sequence files, perform sequence matching to the MicroSEQ® ID-validated reference database and create Neighbor-Joining trees. Moreover, the genera of fungi isolated from the soil were identified using microscopic methods. Municipal sewage sludge can serve as a habitat for plant pathogens and as a source of pathogen strains for biotechnological applications.

Show MeSH