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Bioavailability of plant pigment phytochemicals in Angelica keiskei in older adults: A pilot absorption kinetic study.

Correa CR, Chen CY, Aldini G, Rasmussen H, Ronchi CF, Berchieri-Ronchi C, Cho SM, Blumberg JB, Yeum KJ - Nutr Res Pract (2014)

Bottom Line: Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids.Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry.Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.

View Article: PubMed Central - PubMed

Affiliation: Jean Mayer USDA- Human Nutrition Research Center on Aging, Tufts University, 711 Washington St., Boston, MA 02111, USA. ; Department of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Distrito Rubiao Jr. s/n, 18618-970 Botucatu, SP, Brazil.

ABSTRACT

Background/objectives: Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids. This study examined bioavailability of flavonoids and carotenoids in Angelica keiskei and the alteration of the antioxidant performance in vivo.

Subjects and materials: Absorption kinetics of phytochemicals in Angelica keiskei were determined in healthy older adults (> 60 y, n = 5) and subjects with metabolic syndrome (n = 5). Subjects consumed 5 g dry Angelica keiskei powder encapsulated in gelatin capsules with a low flavonoid and carotenoid liquid meal. Plasma samples were collected at baseline, 0.5, 1, 2, 3, 4, 5, 6, 7, and 8 h. Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry.

Results: After ingestion of Angelica keiskei increases in plasma quercetin concentrations were observed at 1-3 and 6-8 hr in the healthy group and at all time points in the metabolic syndrome group compared to baseline (P < 0.05). Plasma lutein concentrations were significantly elevated in both the healthy and metabolic syndrome groups at 8 hr (P < 0.05). Significant increases in total antioxidant performance were also observed in both the healthy and the metabolic syndrome groups compared to baseline (P < 0.05).

Conclusions: Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.

No MeSH data available.


Related in: MedlinePlus

Absorption kinetics of quercetin in plasma after ingesting 5 g of Angelica keiskei in older adults (aged ≥ 60 yr); healthy (A) and metabolic syndrome (B). Data are expressed as mean ± SEM (n = 5 in each group). ND, not detected. One-way repeated measures ANOVA with Tukey's multiple comparison was performed. When an equal variance test failed, Friedman repeated measures analysis of variance on ranks with Tukey's multiple comparison test was used. Significantly different as compared with the baseline *P < 0.05. Samples were analyzed by HPLC with ECD detection for quercetin.
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Figure 1: Absorption kinetics of quercetin in plasma after ingesting 5 g of Angelica keiskei in older adults (aged ≥ 60 yr); healthy (A) and metabolic syndrome (B). Data are expressed as mean ± SEM (n = 5 in each group). ND, not detected. One-way repeated measures ANOVA with Tukey's multiple comparison was performed. When an equal variance test failed, Friedman repeated measures analysis of variance on ranks with Tukey's multiple comparison test was used. Significantly different as compared with the baseline *P < 0.05. Samples were analyzed by HPLC with ECD detection for quercetin.

Mentions: After ingestion of Angelica keiskei, the plasma quercetin concentrations of the healthy study participants (Fig. 1A) increased significantly at 1, 2, and 3 h as compared to baseline (P < 0.05), decreased after lunch at 4 and 5 h, and increased significantly again at 6 h, maintaining the significantly elevated level until 8 hours (P < 0.05). The plasma quercetin concentrations in the metabolic syndrome group were also significantly increased (P < 0.05) from the baseline throughout all periods (Fig. 1B). Changes of plasma lutein concentrations in the healthy (Fig. 2A) and metabolic syndrome groups are shown in Fig. 2 (Fig. 2B). The plasma lutein concentrations were significantly increased (P < 0.05) at 8 h in both healthy and metabolic syndrome groups as compared to those of the baseline after ingesting 5g of Angelica keiskei dry powder.


Bioavailability of plant pigment phytochemicals in Angelica keiskei in older adults: A pilot absorption kinetic study.

Correa CR, Chen CY, Aldini G, Rasmussen H, Ronchi CF, Berchieri-Ronchi C, Cho SM, Blumberg JB, Yeum KJ - Nutr Res Pract (2014)

Absorption kinetics of quercetin in plasma after ingesting 5 g of Angelica keiskei in older adults (aged ≥ 60 yr); healthy (A) and metabolic syndrome (B). Data are expressed as mean ± SEM (n = 5 in each group). ND, not detected. One-way repeated measures ANOVA with Tukey's multiple comparison was performed. When an equal variance test failed, Friedman repeated measures analysis of variance on ranks with Tukey's multiple comparison test was used. Significantly different as compared with the baseline *P < 0.05. Samples were analyzed by HPLC with ECD detection for quercetin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4198969&req=5

Figure 1: Absorption kinetics of quercetin in plasma after ingesting 5 g of Angelica keiskei in older adults (aged ≥ 60 yr); healthy (A) and metabolic syndrome (B). Data are expressed as mean ± SEM (n = 5 in each group). ND, not detected. One-way repeated measures ANOVA with Tukey's multiple comparison was performed. When an equal variance test failed, Friedman repeated measures analysis of variance on ranks with Tukey's multiple comparison test was used. Significantly different as compared with the baseline *P < 0.05. Samples were analyzed by HPLC with ECD detection for quercetin.
Mentions: After ingestion of Angelica keiskei, the plasma quercetin concentrations of the healthy study participants (Fig. 1A) increased significantly at 1, 2, and 3 h as compared to baseline (P < 0.05), decreased after lunch at 4 and 5 h, and increased significantly again at 6 h, maintaining the significantly elevated level until 8 hours (P < 0.05). The plasma quercetin concentrations in the metabolic syndrome group were also significantly increased (P < 0.05) from the baseline throughout all periods (Fig. 1B). Changes of plasma lutein concentrations in the healthy (Fig. 2A) and metabolic syndrome groups are shown in Fig. 2 (Fig. 2B). The plasma lutein concentrations were significantly increased (P < 0.05) at 8 h in both healthy and metabolic syndrome groups as compared to those of the baseline after ingesting 5g of Angelica keiskei dry powder.

Bottom Line: Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids.Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry.Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.

View Article: PubMed Central - PubMed

Affiliation: Jean Mayer USDA- Human Nutrition Research Center on Aging, Tufts University, 711 Washington St., Boston, MA 02111, USA. ; Department of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Distrito Rubiao Jr. s/n, 18618-970 Botucatu, SP, Brazil.

ABSTRACT

Background/objectives: Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids. This study examined bioavailability of flavonoids and carotenoids in Angelica keiskei and the alteration of the antioxidant performance in vivo.

Subjects and materials: Absorption kinetics of phytochemicals in Angelica keiskei were determined in healthy older adults (> 60 y, n = 5) and subjects with metabolic syndrome (n = 5). Subjects consumed 5 g dry Angelica keiskei powder encapsulated in gelatin capsules with a low flavonoid and carotenoid liquid meal. Plasma samples were collected at baseline, 0.5, 1, 2, 3, 4, 5, 6, 7, and 8 h. Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry.

Results: After ingestion of Angelica keiskei increases in plasma quercetin concentrations were observed at 1-3 and 6-8 hr in the healthy group and at all time points in the metabolic syndrome group compared to baseline (P < 0.05). Plasma lutein concentrations were significantly elevated in both the healthy and metabolic syndrome groups at 8 hr (P < 0.05). Significant increases in total antioxidant performance were also observed in both the healthy and the metabolic syndrome groups compared to baseline (P < 0.05).

Conclusions: Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.

No MeSH data available.


Related in: MedlinePlus