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Carnosic acid inhibits TLR4-MyD88 signaling pathway in LPS-stimulated 3T3-L1 adipocytes.

Park MY, Mun ST - Nutr Res Pract (2014)

Bottom Line: Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities.LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-κB, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA.Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Food & Nutrition Education, Graduate School of Education, Soonchunhyang University, Asan, Chungnam 336-745, Korea.

ABSTRACT

Background/objectives: Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities. However, whether its anti-inflammatory potency can contribute to the amelioration of obesity has not been elucidated. The aim of the current study was to investigate the effect of CA on Toll-like receptor 4 (TLR4) pathways in the presence of lipopolysaccharide (LPS) in 3T3-L1 adipocytes.

Materials/methods: 3T3-L1 adipocytes were treated with CA (0-20 µM) for 1 h, followed by treatment with LPS for 30 min; mRNA expression of adipokines and protein expression of TLR4-related molecules were then measured.

Results: LPS-stimulated 3T3-L1 adipocytes showed elevated mRNA expression of tumor necrosis factor (TNF)-α, interleukin-6, and monocyte chemoattractant protein-1, and CA significantly inhibited the expression of these adipokine genes. LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-κB, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA.

Conclusions: Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.

No MeSH data available.


Related in: MedlinePlus

Effect of CA on viability of differentiated 3T3-L1 cells. Differentiated 3T3-L1 cells were pretreated with various concentrations of CA for 1 h and then stimulated with or without LPS (100 ng/ml) for 30 min. After incubation, cell viability was assessed using the MTT method. Values are expressed as mean ± SD (n = 4).
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Figure 2: Effect of CA on viability of differentiated 3T3-L1 cells. Differentiated 3T3-L1 cells were pretreated with various concentrations of CA for 1 h and then stimulated with or without LPS (100 ng/ml) for 30 min. After incubation, cell viability was assessed using the MTT method. Values are expressed as mean ± SD (n = 4).

Mentions: Cell viability was assessed by MTT assay. As shown Fig. 2, CA in the concentrations used had no effect on viability of 3T3-L1 cells with or without LPS treatment.


Carnosic acid inhibits TLR4-MyD88 signaling pathway in LPS-stimulated 3T3-L1 adipocytes.

Park MY, Mun ST - Nutr Res Pract (2014)

Effect of CA on viability of differentiated 3T3-L1 cells. Differentiated 3T3-L1 cells were pretreated with various concentrations of CA for 1 h and then stimulated with or without LPS (100 ng/ml) for 30 min. After incubation, cell viability was assessed using the MTT method. Values are expressed as mean ± SD (n = 4).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4198963&req=5

Figure 2: Effect of CA on viability of differentiated 3T3-L1 cells. Differentiated 3T3-L1 cells were pretreated with various concentrations of CA for 1 h and then stimulated with or without LPS (100 ng/ml) for 30 min. After incubation, cell viability was assessed using the MTT method. Values are expressed as mean ± SD (n = 4).
Mentions: Cell viability was assessed by MTT assay. As shown Fig. 2, CA in the concentrations used had no effect on viability of 3T3-L1 cells with or without LPS treatment.

Bottom Line: Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities.LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-κB, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA.Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Food & Nutrition Education, Graduate School of Education, Soonchunhyang University, Asan, Chungnam 336-745, Korea.

ABSTRACT

Background/objectives: Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities. However, whether its anti-inflammatory potency can contribute to the amelioration of obesity has not been elucidated. The aim of the current study was to investigate the effect of CA on Toll-like receptor 4 (TLR4) pathways in the presence of lipopolysaccharide (LPS) in 3T3-L1 adipocytes.

Materials/methods: 3T3-L1 adipocytes were treated with CA (0-20 µM) for 1 h, followed by treatment with LPS for 30 min; mRNA expression of adipokines and protein expression of TLR4-related molecules were then measured.

Results: LPS-stimulated 3T3-L1 adipocytes showed elevated mRNA expression of tumor necrosis factor (TNF)-α, interleukin-6, and monocyte chemoattractant protein-1, and CA significantly inhibited the expression of these adipokine genes. LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-κB, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA.

Conclusions: Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.

No MeSH data available.


Related in: MedlinePlus