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Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP Kinase.

Lee JE, Cho SM, Park E, Lee SM, Kim Y, Auh JH, Choi HK, Lim S, Lee SC, Kim JH - Nutr Res Pract (2014)

Bottom Line: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions.Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation.These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Home Economics Education, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea.

ABSTRACT

Background/objectives: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.

Materials/methods: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.

Results: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.

Conclusions: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.

No MeSH data available.


Related in: MedlinePlus

Effects of Rubus coreanus Miquel (RCM) extracts on cell viability in RAW 264.7. Cells were treated with UE, UH, RE and RH (0, 12.5, 25, 50, 100, 200 and 400 µg/ml) for 24 h then percentage of cell viability was measured by MTT assay. Values not sharing the same letter were significantly different (P < 0.05). (UE: unripe Rubus coreanus Miquel ethanol extract, UH: unripe Rubus coreanus Miquel water extract, RE: ripe Rubus coreanus Miquel ethanol extract, and RH: ripe Rubus coreanus Miquel water extract)
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Figure 1: Effects of Rubus coreanus Miquel (RCM) extracts on cell viability in RAW 264.7. Cells were treated with UE, UH, RE and RH (0, 12.5, 25, 50, 100, 200 and 400 µg/ml) for 24 h then percentage of cell viability was measured by MTT assay. Values not sharing the same letter were significantly different (P < 0.05). (UE: unripe Rubus coreanus Miquel ethanol extract, UH: unripe Rubus coreanus Miquel water extract, RE: ripe Rubus coreanus Miquel ethanol extract, and RH: ripe Rubus coreanus Miquel water extract)

Mentions: To avoid any cytotoxic effect caused by RCM, we investigated the effects of RCM extracts on cell viability in RAW 264.7 using MTT assays (Fig. 1). RCM extract had no effect on cell viability except for UE, which slightly increased cell viability at 200 µg/ml. RAW 264.7 were treated with 400 µg/ml of RCM for subsequent analyses.


Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP Kinase.

Lee JE, Cho SM, Park E, Lee SM, Kim Y, Auh JH, Choi HK, Lim S, Lee SC, Kim JH - Nutr Res Pract (2014)

Effects of Rubus coreanus Miquel (RCM) extracts on cell viability in RAW 264.7. Cells were treated with UE, UH, RE and RH (0, 12.5, 25, 50, 100, 200 and 400 µg/ml) for 24 h then percentage of cell viability was measured by MTT assay. Values not sharing the same letter were significantly different (P < 0.05). (UE: unripe Rubus coreanus Miquel ethanol extract, UH: unripe Rubus coreanus Miquel water extract, RE: ripe Rubus coreanus Miquel ethanol extract, and RH: ripe Rubus coreanus Miquel water extract)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4198961&req=5

Figure 1: Effects of Rubus coreanus Miquel (RCM) extracts on cell viability in RAW 264.7. Cells were treated with UE, UH, RE and RH (0, 12.5, 25, 50, 100, 200 and 400 µg/ml) for 24 h then percentage of cell viability was measured by MTT assay. Values not sharing the same letter were significantly different (P < 0.05). (UE: unripe Rubus coreanus Miquel ethanol extract, UH: unripe Rubus coreanus Miquel water extract, RE: ripe Rubus coreanus Miquel ethanol extract, and RH: ripe Rubus coreanus Miquel water extract)
Mentions: To avoid any cytotoxic effect caused by RCM, we investigated the effects of RCM extracts on cell viability in RAW 264.7 using MTT assays (Fig. 1). RCM extract had no effect on cell viability except for UE, which slightly increased cell viability at 200 µg/ml. RAW 264.7 were treated with 400 µg/ml of RCM for subsequent analyses.

Bottom Line: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions.Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation.These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Home Economics Education, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea.

ABSTRACT

Background/objectives: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.

Materials/methods: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.

Results: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.

Conclusions: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.

No MeSH data available.


Related in: MedlinePlus