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TRPV Channels in Mast Cells as a Target for Low-Level-Laser Therapy.

Wang L, Zhang D, Schwarz W - Cells (2014)

Bottom Line: Here we summarize and discuss effects of laser irradiation on mast cells that leads to degranulation of the cells.This process may contribute to initial steps in the final medical effects.We suggest that activation of TRPV channels in the mast cells forms a basis for the underlying mechanisms and that released ATP and histamine may be putative mediators for therapeutic effects.

View Article: PubMed Central - PubMed

Affiliation: Shanghai University of Traditional Chinese Medicine and Shanghai Research Center for Acupuncture and Meridians, Shanghai 201203, China. linawang1103@163.com.

ABSTRACT
Low-level laser irradiation in the visible as well as infrared range is applied to skin for treatment of various diseases. Here we summarize and discuss effects of laser irradiation on mast cells that leads to degranulation of the cells. This process may contribute to initial steps in the final medical effects. We suggest that activation of TRPV channels in the mast cells forms a basis for the underlying mechanisms and that released ATP and histamine may be putative mediators for therapeutic effects.

No MeSH data available.


Related in: MedlinePlus

Changes in [Ca2+]i and extracellular ATP in HMC-1 cells in response to noxious heat that activates TRPV1 (43 °C) and TRPV2 (52 °C), and either in the absence (dark grey bars) or presence (light grey bars) of 5 mM EGTA. (A) Quantitative analysis from several sets of experiments normalized to basal [Ca2+]i–dependent fluorescence at 28 °C (=100% of Calcium Green-1 loaded HMC-1 cells). Data represent averages ± SEM from N = 3–4 independent experiments (* p < 0.05, ** p < 0.01, compared to 28 °C; $$ p < 0.01 compare to 43 °C, ## p < 0.01, compared to 52 °C); (B) ATP content in cell suspension after 3 min of incubation at the respective temperatures. Data represent averages ±SEM from n = 6–42 samples of N = 3–11 independent experiments (* p = < 0.05 and ** p < 0.0001, compared to control; ## p < 0.0001, compare to 52 °C). Data in (A) are unpublished, data in (B) are based on Hu et al. 2014 [26].
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cells-03-00662-f004: Changes in [Ca2+]i and extracellular ATP in HMC-1 cells in response to noxious heat that activates TRPV1 (43 °C) and TRPV2 (52 °C), and either in the absence (dark grey bars) or presence (light grey bars) of 5 mM EGTA. (A) Quantitative analysis from several sets of experiments normalized to basal [Ca2+]i–dependent fluorescence at 28 °C (=100% of Calcium Green-1 loaded HMC-1 cells). Data represent averages ± SEM from N = 3–4 independent experiments (* p < 0.05, ** p < 0.01, compared to 28 °C; $$ p < 0.01 compare to 43 °C, ## p < 0.01, compared to 52 °C); (B) ATP content in cell suspension after 3 min of incubation at the respective temperatures. Data represent averages ±SEM from n = 6–42 samples of N = 3–11 independent experiments (* p = < 0.05 and ** p < 0.0001, compared to control; ## p < 0.0001, compare to 52 °C). Data in (A) are unpublished, data in (B) are based on Hu et al. 2014 [26].

Mentions: The thermal activation of TRPV1 and TRPV2 is characterized by threshold temperatures of 43 °C and 52 °C, respectively. These noxious temperatures resulted in TRPV1- and TRPV2-dependent influx of Ca2+ in HMC-1 leading to elevated [Ca2+]i (Figure 4A). Only exceeding a temperature of 43 °C increased [Ca2+]i, and a further significant increase was observed when exceeding 52 °C. The relative changes in intracellular calcium activity were monitored by measuring the [Ca2+]i–dependent fluorescence of Calcium Green. The fluorescence signal of Calcium Green was reported to be temperature-dependent [34,35], but at the temperatures exceeding 28 °C the signal is hardly affected, and the changes illustrated in Figure 4A will not significantly be altered. As was illustrated for stimulation of mast cells by red laser light (see above), the temperature-induced elevation of [Ca2+]i triggers release of ATP, which becomes apparent in elevated extracellular ATP content (Figure 4B).


TRPV Channels in Mast Cells as a Target for Low-Level-Laser Therapy.

Wang L, Zhang D, Schwarz W - Cells (2014)

Changes in [Ca2+]i and extracellular ATP in HMC-1 cells in response to noxious heat that activates TRPV1 (43 °C) and TRPV2 (52 °C), and either in the absence (dark grey bars) or presence (light grey bars) of 5 mM EGTA. (A) Quantitative analysis from several sets of experiments normalized to basal [Ca2+]i–dependent fluorescence at 28 °C (=100% of Calcium Green-1 loaded HMC-1 cells). Data represent averages ± SEM from N = 3–4 independent experiments (* p < 0.05, ** p < 0.01, compared to 28 °C; $$ p < 0.01 compare to 43 °C, ## p < 0.01, compared to 52 °C); (B) ATP content in cell suspension after 3 min of incubation at the respective temperatures. Data represent averages ±SEM from n = 6–42 samples of N = 3–11 independent experiments (* p = < 0.05 and ** p < 0.0001, compared to control; ## p < 0.0001, compare to 52 °C). Data in (A) are unpublished, data in (B) are based on Hu et al. 2014 [26].
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4197630&req=5

cells-03-00662-f004: Changes in [Ca2+]i and extracellular ATP in HMC-1 cells in response to noxious heat that activates TRPV1 (43 °C) and TRPV2 (52 °C), and either in the absence (dark grey bars) or presence (light grey bars) of 5 mM EGTA. (A) Quantitative analysis from several sets of experiments normalized to basal [Ca2+]i–dependent fluorescence at 28 °C (=100% of Calcium Green-1 loaded HMC-1 cells). Data represent averages ± SEM from N = 3–4 independent experiments (* p < 0.05, ** p < 0.01, compared to 28 °C; $$ p < 0.01 compare to 43 °C, ## p < 0.01, compared to 52 °C); (B) ATP content in cell suspension after 3 min of incubation at the respective temperatures. Data represent averages ±SEM from n = 6–42 samples of N = 3–11 independent experiments (* p = < 0.05 and ** p < 0.0001, compared to control; ## p < 0.0001, compare to 52 °C). Data in (A) are unpublished, data in (B) are based on Hu et al. 2014 [26].
Mentions: The thermal activation of TRPV1 and TRPV2 is characterized by threshold temperatures of 43 °C and 52 °C, respectively. These noxious temperatures resulted in TRPV1- and TRPV2-dependent influx of Ca2+ in HMC-1 leading to elevated [Ca2+]i (Figure 4A). Only exceeding a temperature of 43 °C increased [Ca2+]i, and a further significant increase was observed when exceeding 52 °C. The relative changes in intracellular calcium activity were monitored by measuring the [Ca2+]i–dependent fluorescence of Calcium Green. The fluorescence signal of Calcium Green was reported to be temperature-dependent [34,35], but at the temperatures exceeding 28 °C the signal is hardly affected, and the changes illustrated in Figure 4A will not significantly be altered. As was illustrated for stimulation of mast cells by red laser light (see above), the temperature-induced elevation of [Ca2+]i triggers release of ATP, which becomes apparent in elevated extracellular ATP content (Figure 4B).

Bottom Line: Here we summarize and discuss effects of laser irradiation on mast cells that leads to degranulation of the cells.This process may contribute to initial steps in the final medical effects.We suggest that activation of TRPV channels in the mast cells forms a basis for the underlying mechanisms and that released ATP and histamine may be putative mediators for therapeutic effects.

View Article: PubMed Central - PubMed

Affiliation: Shanghai University of Traditional Chinese Medicine and Shanghai Research Center for Acupuncture and Meridians, Shanghai 201203, China. linawang1103@163.com.

ABSTRACT
Low-level laser irradiation in the visible as well as infrared range is applied to skin for treatment of various diseases. Here we summarize and discuss effects of laser irradiation on mast cells that leads to degranulation of the cells. This process may contribute to initial steps in the final medical effects. We suggest that activation of TRPV channels in the mast cells forms a basis for the underlying mechanisms and that released ATP and histamine may be putative mediators for therapeutic effects.

No MeSH data available.


Related in: MedlinePlus