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TRPV Channels in Mast Cells as a Target for Low-Level-Laser Therapy.

Wang L, Zhang D, Schwarz W - Cells (2014)

Bottom Line: Low-level laser irradiation in the visible as well as infrared range is applied to skin for treatment of various diseases.This process may contribute to initial steps in the final medical effects.We suggest that activation of TRPV channels in the mast cells forms a basis for the underlying mechanisms and that released ATP and histamine may be putative mediators for therapeutic effects.

View Article: PubMed Central - PubMed

Affiliation: Shanghai University of Traditional Chinese Medicine and Shanghai Research Center for Acupuncture and Meridians, Shanghai 201203, China. linawang1103@163.com.

ABSTRACT
Low-level laser irradiation in the visible as well as infrared range is applied to skin for treatment of various diseases. Here we summarize and discuss effects of laser irradiation on mast cells that leads to degranulation of the cells. This process may contribute to initial steps in the final medical effects. We suggest that activation of TRPV channels in the mast cells forms a basis for the underlying mechanisms and that released ATP and histamine may be putative mediators for therapeutic effects.

No MeSH data available.


Related in: MedlinePlus

Changes in [Ca2+]i and extracellular ATP in HMC-1 cells in response to 657 nm and 280 mW/cm2 laser irradiation for 3 min. (A) Quantitative analysis of several sets of cells normalized to basal [Ca2+]i-dependent fluorescence before irradiation (=100%). Data represent averages ± SEM (n = 15 cells, *p < 0.01 compared to control); (B) Relative changes of extracellular ATP averaged from all sample. The data represent averages ± SEM (n = 27, *p < 0.01 compared to control, and are based on Wang et al. 2013 [33]).
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cells-03-00662-f003: Changes in [Ca2+]i and extracellular ATP in HMC-1 cells in response to 657 nm and 280 mW/cm2 laser irradiation for 3 min. (A) Quantitative analysis of several sets of cells normalized to basal [Ca2+]i-dependent fluorescence before irradiation (=100%). Data represent averages ± SEM (n = 15 cells, *p < 0.01 compared to control); (B) Relative changes of extracellular ATP averaged from all sample. The data represent averages ± SEM (n = 27, *p < 0.01 compared to control, and are based on Wang et al. 2013 [33]).

Mentions: Since TRPV channels are permeable for Ca2+, Ca2+ influx can be expected to occur in response to TRPV channel activation. Concerning HMC-1 cells, intracellular calcium activity ([Ca2+]i) indeed increased in response to irradiation by red laser light of 657 nm (280 mW/cm2, 54 × 103 mJ/cm2) (Figure 3A). Interestingly, this elevation of [Ca2+]i was independent on extracellular Ca2+. With the same irradiation parameters that stimulate [Ca2+]i increase an elevation of extracellular ATP could be detected (Figure 3B), and the [Ca2+]i was demonstrated to trigger exocytotic release of ATP [32].


TRPV Channels in Mast Cells as a Target for Low-Level-Laser Therapy.

Wang L, Zhang D, Schwarz W - Cells (2014)

Changes in [Ca2+]i and extracellular ATP in HMC-1 cells in response to 657 nm and 280 mW/cm2 laser irradiation for 3 min. (A) Quantitative analysis of several sets of cells normalized to basal [Ca2+]i-dependent fluorescence before irradiation (=100%). Data represent averages ± SEM (n = 15 cells, *p < 0.01 compared to control); (B) Relative changes of extracellular ATP averaged from all sample. The data represent averages ± SEM (n = 27, *p < 0.01 compared to control, and are based on Wang et al. 2013 [33]).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197630&req=5

cells-03-00662-f003: Changes in [Ca2+]i and extracellular ATP in HMC-1 cells in response to 657 nm and 280 mW/cm2 laser irradiation for 3 min. (A) Quantitative analysis of several sets of cells normalized to basal [Ca2+]i-dependent fluorescence before irradiation (=100%). Data represent averages ± SEM (n = 15 cells, *p < 0.01 compared to control); (B) Relative changes of extracellular ATP averaged from all sample. The data represent averages ± SEM (n = 27, *p < 0.01 compared to control, and are based on Wang et al. 2013 [33]).
Mentions: Since TRPV channels are permeable for Ca2+, Ca2+ influx can be expected to occur in response to TRPV channel activation. Concerning HMC-1 cells, intracellular calcium activity ([Ca2+]i) indeed increased in response to irradiation by red laser light of 657 nm (280 mW/cm2, 54 × 103 mJ/cm2) (Figure 3A). Interestingly, this elevation of [Ca2+]i was independent on extracellular Ca2+. With the same irradiation parameters that stimulate [Ca2+]i increase an elevation of extracellular ATP could be detected (Figure 3B), and the [Ca2+]i was demonstrated to trigger exocytotic release of ATP [32].

Bottom Line: Low-level laser irradiation in the visible as well as infrared range is applied to skin for treatment of various diseases.This process may contribute to initial steps in the final medical effects.We suggest that activation of TRPV channels in the mast cells forms a basis for the underlying mechanisms and that released ATP and histamine may be putative mediators for therapeutic effects.

View Article: PubMed Central - PubMed

Affiliation: Shanghai University of Traditional Chinese Medicine and Shanghai Research Center for Acupuncture and Meridians, Shanghai 201203, China. linawang1103@163.com.

ABSTRACT
Low-level laser irradiation in the visible as well as infrared range is applied to skin for treatment of various diseases. Here we summarize and discuss effects of laser irradiation on mast cells that leads to degranulation of the cells. This process may contribute to initial steps in the final medical effects. We suggest that activation of TRPV channels in the mast cells forms a basis for the underlying mechanisms and that released ATP and histamine may be putative mediators for therapeutic effects.

No MeSH data available.


Related in: MedlinePlus