Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Bottom Line: The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes.The cell lineage specificity of reporter gene expression was confirmed by demonstration of coincident expression with the endogenous CSF1R protein.In transgenic birds, expression of the reporter gene provided a defined marker for macrophage-lineage cells, identifying the earliest stages in the yolk sac, throughout embryonic development and in all adult tissues.
Affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Sciences, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK firstname.lastname@example.org email@example.com.Show MeSH
Related in: MedlinePlus
Mentions: The expression of CSF1R-transgene expression in chicken tissue mononuclear subsets in the lymphoid organs and non-lymphoid tissues was examined by confocal microscopy. In the spleen, CSF1R-transgene-expressing cells were abundant and found in association with B-cells of the peri-ellipsoid lymphocyte sheath (PELS) and within the ellipsoid (Fig. 5A), consistent with previous studies of splenic macrophage populations (Jeurissen et al., 1989; Nagy et al., 2005; Igyártó et al., 2007). In the bursa of Fabricius, the avian-specific primary lymphoid organ for B-cell production, CSF1R-transgene-expressing cells were found in the medulla of B-cell follicles and in the interfollicular tissues (Fig. 5B). The location of CSF1R-transgene-expressing cells in the medulla is consistent with their identity as bursal secretory dendritic cells (BSDCs) (Oláh et al., 1992). Dense networks of CSF1R-transgene-expressing cells were present in the medulla region of germinal centres in the caecal tonsil (Fig. 5C). The distribution of cells in the medulla of germinal centres is consistent with cells previously described as avian follicular dendritic cells (FDCs) (Eikelenboom et al., 1983; Jeurissen, 1993). Both BSDCs and FDCs expressed high levels of CSF1R protein (supplementary material Fig. S4).Fig. 5.
Affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Sciences, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK firstname.lastname@example.org email@example.com.