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Immediate and punitive impact of mechanosensory disturbance on olfactory behaviour of larval Drosophila.

Saumweber T, Cano C, Klessen J, Eichler K, Fendt M, Gerber B - Biol Open (2014)

Bottom Line: We report that (i) during 2-3 seconds after buzz onset the larvae slowed down and then turned, arguably to escape this situation; this was seen for buzz frequencies of 10, 100, and 1000 Hz, (ii) innate olfactory preference was reduced when tested in the presence of the buzz; this effect was strongest for the 100 Hz frequency, (iii) after odour-buzz associative training, we observed escape from the buzz-associated odour; this effect was apparent for 10 and 100, but not for 1000 Hz.We discuss the multiple behavioural effects of mechanosensation and stress that the immediate effects on locomotion and the impact as punishment differ in their frequency-dependence.It should be interesting to see how these features map onto the organization of sensory, ascending pathways.

View Article: PubMed Central - PubMed

Affiliation: Institut für Biologie, Universität Leipzig, Tierphysiologie, 04103 Leipzig, Germany Abteilung Genetik von Lernen und Gedächtnis, Leibniz Institut für Neurobiologie (LIN), 39118 Magdeburg, Germany timo.saumweber@lin-magdeburg.de bertram.gerber@lin-magdeburg.de.

No MeSH data available.


Related in: MedlinePlus

Buzz as modulator of olfactory preference.(A,B) Time course of olfactory preference. Larvae were offered the choice between an odour side and a blank side of a Petri dish. Preference was scored after 1 min, 3 min, and 5 min, using either n-amyl acetate (AM) or 1-octanol (OCT) as odours. For both odours, choice behaviour reached a steady state after 5 min; therefore these 5-min scores are displayed in panels C and D. * indicates significance from zero (OSS-tests, P<0.05/3) (N = 24 for AM and N = 23 for OCT preferences). (C,D) Modulation of innate olfactory preference behaviour by the buzz. Odour preferences, for AM or for OCT, are displayed, either assayed without the buzz, or assayed in the presence of the buzz at the indicated frequency. Buzzes of 100 Hz abolish innate olfactory behaviour. * and ns refer to P<0.05/4 and P>0.05/4, respectively (OSS-tests) (N = 24, 19, 19, 18; N = 23, 19, 18, 18).
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f03: Buzz as modulator of olfactory preference.(A,B) Time course of olfactory preference. Larvae were offered the choice between an odour side and a blank side of a Petri dish. Preference was scored after 1 min, 3 min, and 5 min, using either n-amyl acetate (AM) or 1-octanol (OCT) as odours. For both odours, choice behaviour reached a steady state after 5 min; therefore these 5-min scores are displayed in panels C and D. * indicates significance from zero (OSS-tests, P<0.05/3) (N = 24 for AM and N = 23 for OCT preferences). (C,D) Modulation of innate olfactory preference behaviour by the buzz. Odour preferences, for AM or for OCT, are displayed, either assayed without the buzz, or assayed in the presence of the buzz at the indicated frequency. Buzzes of 100 Hz abolish innate olfactory behaviour. * and ns refer to P<0.05/4 and P>0.05/4, respectively (OSS-tests) (N = 24, 19, 19, 18; N = 23, 19, 18, 18).

Mentions: We offered the larvae a choice between an odour side (either AM or OCT) versus a blank side of a Petri dish and recorded their preference – and did so either in the presence or in the absence of a buzz (Fig. 3A,C). Given that it takes 3–5 min for the larvae to distribute themselves between both sides of the Petri dish (Fig. 3B), we chose to focus on the data at 5 min. This was done for either 10, 100, or 1000 Hz buzzes. In the presence of 10 Hz and 1000 Hz buzzes the larvae behaved the same as larvae tested without a buzz; to our surprise, however, in the presence of 100 Hz buzzes innate odour preference was strongly decreased, for both the odours employed (Fig. 3D).


Immediate and punitive impact of mechanosensory disturbance on olfactory behaviour of larval Drosophila.

Saumweber T, Cano C, Klessen J, Eichler K, Fendt M, Gerber B - Biol Open (2014)

Buzz as modulator of olfactory preference.(A,B) Time course of olfactory preference. Larvae were offered the choice between an odour side and a blank side of a Petri dish. Preference was scored after 1 min, 3 min, and 5 min, using either n-amyl acetate (AM) or 1-octanol (OCT) as odours. For both odours, choice behaviour reached a steady state after 5 min; therefore these 5-min scores are displayed in panels C and D. * indicates significance from zero (OSS-tests, P<0.05/3) (N = 24 for AM and N = 23 for OCT preferences). (C,D) Modulation of innate olfactory preference behaviour by the buzz. Odour preferences, for AM or for OCT, are displayed, either assayed without the buzz, or assayed in the presence of the buzz at the indicated frequency. Buzzes of 100 Hz abolish innate olfactory behaviour. * and ns refer to P<0.05/4 and P>0.05/4, respectively (OSS-tests) (N = 24, 19, 19, 18; N = 23, 19, 18, 18).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197435&req=5

f03: Buzz as modulator of olfactory preference.(A,B) Time course of olfactory preference. Larvae were offered the choice between an odour side and a blank side of a Petri dish. Preference was scored after 1 min, 3 min, and 5 min, using either n-amyl acetate (AM) or 1-octanol (OCT) as odours. For both odours, choice behaviour reached a steady state after 5 min; therefore these 5-min scores are displayed in panels C and D. * indicates significance from zero (OSS-tests, P<0.05/3) (N = 24 for AM and N = 23 for OCT preferences). (C,D) Modulation of innate olfactory preference behaviour by the buzz. Odour preferences, for AM or for OCT, are displayed, either assayed without the buzz, or assayed in the presence of the buzz at the indicated frequency. Buzzes of 100 Hz abolish innate olfactory behaviour. * and ns refer to P<0.05/4 and P>0.05/4, respectively (OSS-tests) (N = 24, 19, 19, 18; N = 23, 19, 18, 18).
Mentions: We offered the larvae a choice between an odour side (either AM or OCT) versus a blank side of a Petri dish and recorded their preference – and did so either in the presence or in the absence of a buzz (Fig. 3A,C). Given that it takes 3–5 min for the larvae to distribute themselves between both sides of the Petri dish (Fig. 3B), we chose to focus on the data at 5 min. This was done for either 10, 100, or 1000 Hz buzzes. In the presence of 10 Hz and 1000 Hz buzzes the larvae behaved the same as larvae tested without a buzz; to our surprise, however, in the presence of 100 Hz buzzes innate odour preference was strongly decreased, for both the odours employed (Fig. 3D).

Bottom Line: We report that (i) during 2-3 seconds after buzz onset the larvae slowed down and then turned, arguably to escape this situation; this was seen for buzz frequencies of 10, 100, and 1000 Hz, (ii) innate olfactory preference was reduced when tested in the presence of the buzz; this effect was strongest for the 100 Hz frequency, (iii) after odour-buzz associative training, we observed escape from the buzz-associated odour; this effect was apparent for 10 and 100, but not for 1000 Hz.We discuss the multiple behavioural effects of mechanosensation and stress that the immediate effects on locomotion and the impact as punishment differ in their frequency-dependence.It should be interesting to see how these features map onto the organization of sensory, ascending pathways.

View Article: PubMed Central - PubMed

Affiliation: Institut für Biologie, Universität Leipzig, Tierphysiologie, 04103 Leipzig, Germany Abteilung Genetik von Lernen und Gedächtnis, Leibniz Institut für Neurobiologie (LIN), 39118 Magdeburg, Germany timo.saumweber@lin-magdeburg.de bertram.gerber@lin-magdeburg.de.

No MeSH data available.


Related in: MedlinePlus