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High expression of MAGE-A9 correlates with unfavorable survival in hepatocellular carcinoma.

Gu X, Fu M, Ge Z, Zhan F, Ding Y, Ni H, Zhang W, Zhu Y, Tang X, Xiong L, Li J, Qiu L, Mao Y, Zhu J - Sci Rep (2014)

Bottom Line: The results showed that the expression of MAGE-A9 in HCC was significantly higher than that in non-cancerous cells and tissues.Cox regression analysis further revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (p = 0.006) and overall survival (p = 0.022).These data are the first to indicate that MAGE-A9 expression is a valuable prognostic biomarker for HCC and that high MAGE-A9 expression suggests unfavorable survival outcomes in HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.

ABSTRACT
Melanoma-associated antigens (MAGE)-A9 has been reported to play important roles in the development of human cancers. However, the association between MAGE-A9 expression and the clinicopathological characteristics of hepatocellular carcinoma (HCC) is not well understood. The study was to detect the expression of MAGE-A9 in human HCC and investigate the association between its expression and the clinicopathological characteristics of HCC. Reverse transcription-polymerase chain reaction (RT-PCR), one-step quantitative -PCR (qPCR) and immunohistochemistry (IHC) analyses were performed to characterize the expression of MAGE-A9 in HCC cell lines and tissues. Kaplan-Meier survival and Cox regression analyses were employed to evaluate the prognosis of 100 HCC patients. The results showed that the expression of MAGE-A9 in HCC was significantly higher than that in non-cancerous cells and tissues. Moreover, the expression level of the MAGE-A9 protein in HCC was related to the pathological grade (p = 0.003), portal vein invasion (p = 0.001), distant metastasis (p = 0.022) and TNM stage (p = 0.005). Cox regression analysis further revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (p = 0.006) and overall survival (p = 0.022). These data are the first to indicate that MAGE-A9 expression is a valuable prognostic biomarker for HCC and that high MAGE-A9 expression suggests unfavorable survival outcomes in HCC patients.

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Survival analysis of 100 HCC patients by Kaplan-Meier method.(A) Disease-free survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). (B) Disease-free survival rate in patients with positive lymph node metastasis (green line) was significantly lower than that in patients with negative lymph node metastasis (blue line). (C) Overall survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). (D) Overall survival rate in patients with positive portal vein invasion (green line) was significantly lower than that in patients with negative portal vein invasion (blue line).
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f4: Survival analysis of 100 HCC patients by Kaplan-Meier method.(A) Disease-free survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). (B) Disease-free survival rate in patients with positive lymph node metastasis (green line) was significantly lower than that in patients with negative lymph node metastasis (blue line). (C) Overall survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). (D) Overall survival rate in patients with positive portal vein invasion (green line) was significantly lower than that in patients with negative portal vein invasion (blue line).

Mentions: According to a univariate analysis, several factors were correlated with the disease-free survival (DFS) of HCC patients, including MAGE-A9 expression (p = 0.001), tumor size (p = 0.031), pathological grade (p = 0.007), portal vein invasion (p = 0.001), lymph node metastasis (p = 0.001), distant metastasis (p = 0.035) and TNM stage (p = 0.001) (Table 2). All the above items, except for distant metastasis, were also associated with overall survival (OS) in 100 HCC patients (Table 3). By using a multivariate analysis with the Cox regression model, MAGE-A9 expression (p = 0.006) and lymph node metastasis (p = 0.008) indicated a poor DFS, whereas MAGE-A9 expression (p = 0.022) and portal vein invasion (p = 0.039) were identified as independent prognostic factors for OS (Table 2 and 3). Kaplan-Meier survival curves subsequently demonstrated that HCC patients with high MAGE-A9 expression presented a significantly unfavorable DFS time and OS time. In addition, HCC patients with lymph node metastasis and positive portal vein invasion experienced poor DFS and OS rates, respectively (Figure 4).


High expression of MAGE-A9 correlates with unfavorable survival in hepatocellular carcinoma.

Gu X, Fu M, Ge Z, Zhan F, Ding Y, Ni H, Zhang W, Zhu Y, Tang X, Xiong L, Li J, Qiu L, Mao Y, Zhu J - Sci Rep (2014)

Survival analysis of 100 HCC patients by Kaplan-Meier method.(A) Disease-free survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). (B) Disease-free survival rate in patients with positive lymph node metastasis (green line) was significantly lower than that in patients with negative lymph node metastasis (blue line). (C) Overall survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). (D) Overall survival rate in patients with positive portal vein invasion (green line) was significantly lower than that in patients with negative portal vein invasion (blue line).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4197415&req=5

f4: Survival analysis of 100 HCC patients by Kaplan-Meier method.(A) Disease-free survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). (B) Disease-free survival rate in patients with positive lymph node metastasis (green line) was significantly lower than that in patients with negative lymph node metastasis (blue line). (C) Overall survival rate in patients with high MAGE-A9 expression (green line) was significantly lower than that in patients with low MAGE-A9 expression (blue line). (D) Overall survival rate in patients with positive portal vein invasion (green line) was significantly lower than that in patients with negative portal vein invasion (blue line).
Mentions: According to a univariate analysis, several factors were correlated with the disease-free survival (DFS) of HCC patients, including MAGE-A9 expression (p = 0.001), tumor size (p = 0.031), pathological grade (p = 0.007), portal vein invasion (p = 0.001), lymph node metastasis (p = 0.001), distant metastasis (p = 0.035) and TNM stage (p = 0.001) (Table 2). All the above items, except for distant metastasis, were also associated with overall survival (OS) in 100 HCC patients (Table 3). By using a multivariate analysis with the Cox regression model, MAGE-A9 expression (p = 0.006) and lymph node metastasis (p = 0.008) indicated a poor DFS, whereas MAGE-A9 expression (p = 0.022) and portal vein invasion (p = 0.039) were identified as independent prognostic factors for OS (Table 2 and 3). Kaplan-Meier survival curves subsequently demonstrated that HCC patients with high MAGE-A9 expression presented a significantly unfavorable DFS time and OS time. In addition, HCC patients with lymph node metastasis and positive portal vein invasion experienced poor DFS and OS rates, respectively (Figure 4).

Bottom Line: The results showed that the expression of MAGE-A9 in HCC was significantly higher than that in non-cancerous cells and tissues.Cox regression analysis further revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (p = 0.006) and overall survival (p = 0.022).These data are the first to indicate that MAGE-A9 expression is a valuable prognostic biomarker for HCC and that high MAGE-A9 expression suggests unfavorable survival outcomes in HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.

ABSTRACT
Melanoma-associated antigens (MAGE)-A9 has been reported to play important roles in the development of human cancers. However, the association between MAGE-A9 expression and the clinicopathological characteristics of hepatocellular carcinoma (HCC) is not well understood. The study was to detect the expression of MAGE-A9 in human HCC and investigate the association between its expression and the clinicopathological characteristics of HCC. Reverse transcription-polymerase chain reaction (RT-PCR), one-step quantitative -PCR (qPCR) and immunohistochemistry (IHC) analyses were performed to characterize the expression of MAGE-A9 in HCC cell lines and tissues. Kaplan-Meier survival and Cox regression analyses were employed to evaluate the prognosis of 100 HCC patients. The results showed that the expression of MAGE-A9 in HCC was significantly higher than that in non-cancerous cells and tissues. Moreover, the expression level of the MAGE-A9 protein in HCC was related to the pathological grade (p = 0.003), portal vein invasion (p = 0.001), distant metastasis (p = 0.022) and TNM stage (p = 0.005). Cox regression analysis further revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (p = 0.006) and overall survival (p = 0.022). These data are the first to indicate that MAGE-A9 expression is a valuable prognostic biomarker for HCC and that high MAGE-A9 expression suggests unfavorable survival outcomes in HCC patients.

Show MeSH
Related in: MedlinePlus