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Antiproliferatory Effects of Crab Shell Extract on Breast Cancer Cell Line (MCF7).

Rezakhani L, Rashidi Z, Mirzapur P, Khazaei M - J Breast Cancer (2014)

Bottom Line: Cell viability decreased depending on dose and time, and was significantly different in the groups that were treated with 400, 800, and 1,000 µg/mL doses compared to that in the control group (p<0.001).Increasing the dose significantly increased apoptosis (p<0.001).NO secretion from MCF7 cells significantly decreased in response to different concentrations of the extract in a dose- and time-dependent manner (p<0.050).

View Article: PubMed Central - PubMed

Affiliation: Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

ABSTRACT

Purpose: Breast cancer is the most common type of cancer in women. Despite various pharmacological developments, the identification of new therapies is still required for treating breast cancer. Crab is often recommended as a traditional medicine for cancer. This study aimed to determine the in vitro effect of a hydroalcoholic crab shell extract on a breast cancer cell line.

Methods: In this experimental study, MCF7 breast cancer cell line was used. Crab shell was powdered and a hydroalcoholic (70° ethanol) extract was prepared. Five concentrations (100, 200, 400, 800, and 1,000 µg/mL) were added to the cells for three periods, 24, 48, and 72 hours. The viability of the cells were evaluated using trypan blue and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Cell apoptosis was determined using the terminal deoxynucleotidyl transferase dUTP nick end labeling method. Nitric oxide (NO) level was assessed using the Griess method. Data were analyzed using analysis of variance, and p<0.05 was considered significant.

Results: Cell viability decreased depending on dose and time, and was significantly different in the groups that were treated with 400, 800, and 1,000 µg/mL doses compared to that in the control group (p<0.001). Increasing the dose significantly increased apoptosis (p<0.001). NO secretion from MCF7 cells significantly decreased in response to different concentrations of the extract in a dose- and time-dependent manner (p<0.050).

Conclusion: The crab shell extract inhibited the proliferation of MCF7 cells by increasing apoptosis and decreasing NO production.

No MeSH data available.


Related in: MedlinePlus

(A) MCF7 apoptotic index (%) after 72-hour with different amounts of crab shell extract. Significant difference between groups. *p<0.05; †p<0.001. (B) Identification of apoptosis in MCF7 cell line following terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and counterstaining with propidium iodide after 72 hours of exposure crab shell extract. TUNEL positive cells (apoptotic nuclei) identified by a distinct bright yellow stained chromatin (arrows). C+: The positive control was incubated with ethanol 10% for 10 minutes, all cells are seen as bright yellow. C-: The positive control was incubated with label solution without enzyme solution. No TUNEL positive cells were observed (×200).
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Figure 4: (A) MCF7 apoptotic index (%) after 72-hour with different amounts of crab shell extract. Significant difference between groups. *p<0.05; †p<0.001. (B) Identification of apoptosis in MCF7 cell line following terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and counterstaining with propidium iodide after 72 hours of exposure crab shell extract. TUNEL positive cells (apoptotic nuclei) identified by a distinct bright yellow stained chromatin (arrows). C+: The positive control was incubated with ethanol 10% for 10 minutes, all cells are seen as bright yellow. C-: The positive control was incubated with label solution without enzyme solution. No TUNEL positive cells were observed (×200).

Mentions: The apoptosis index of MCF7 cells treated with crab shell extract showed an increase from control to experimental groups in order of concentration, exhibiting a significant difference with 400 (p<0.050), and 800 and 1,000 µg/mL (p<0.001) doses (Figure 4A, B).


Antiproliferatory Effects of Crab Shell Extract on Breast Cancer Cell Line (MCF7).

Rezakhani L, Rashidi Z, Mirzapur P, Khazaei M - J Breast Cancer (2014)

(A) MCF7 apoptotic index (%) after 72-hour with different amounts of crab shell extract. Significant difference between groups. *p<0.05; †p<0.001. (B) Identification of apoptosis in MCF7 cell line following terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and counterstaining with propidium iodide after 72 hours of exposure crab shell extract. TUNEL positive cells (apoptotic nuclei) identified by a distinct bright yellow stained chromatin (arrows). C+: The positive control was incubated with ethanol 10% for 10 minutes, all cells are seen as bright yellow. C-: The positive control was incubated with label solution without enzyme solution. No TUNEL positive cells were observed (×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4197351&req=5

Figure 4: (A) MCF7 apoptotic index (%) after 72-hour with different amounts of crab shell extract. Significant difference between groups. *p<0.05; †p<0.001. (B) Identification of apoptosis in MCF7 cell line following terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and counterstaining with propidium iodide after 72 hours of exposure crab shell extract. TUNEL positive cells (apoptotic nuclei) identified by a distinct bright yellow stained chromatin (arrows). C+: The positive control was incubated with ethanol 10% for 10 minutes, all cells are seen as bright yellow. C-: The positive control was incubated with label solution without enzyme solution. No TUNEL positive cells were observed (×200).
Mentions: The apoptosis index of MCF7 cells treated with crab shell extract showed an increase from control to experimental groups in order of concentration, exhibiting a significant difference with 400 (p<0.050), and 800 and 1,000 µg/mL (p<0.001) doses (Figure 4A, B).

Bottom Line: Cell viability decreased depending on dose and time, and was significantly different in the groups that were treated with 400, 800, and 1,000 µg/mL doses compared to that in the control group (p<0.001).Increasing the dose significantly increased apoptosis (p<0.001).NO secretion from MCF7 cells significantly decreased in response to different concentrations of the extract in a dose- and time-dependent manner (p<0.050).

View Article: PubMed Central - PubMed

Affiliation: Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

ABSTRACT

Purpose: Breast cancer is the most common type of cancer in women. Despite various pharmacological developments, the identification of new therapies is still required for treating breast cancer. Crab is often recommended as a traditional medicine for cancer. This study aimed to determine the in vitro effect of a hydroalcoholic crab shell extract on a breast cancer cell line.

Methods: In this experimental study, MCF7 breast cancer cell line was used. Crab shell was powdered and a hydroalcoholic (70° ethanol) extract was prepared. Five concentrations (100, 200, 400, 800, and 1,000 µg/mL) were added to the cells for three periods, 24, 48, and 72 hours. The viability of the cells were evaluated using trypan blue and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Cell apoptosis was determined using the terminal deoxynucleotidyl transferase dUTP nick end labeling method. Nitric oxide (NO) level was assessed using the Griess method. Data were analyzed using analysis of variance, and p<0.05 was considered significant.

Results: Cell viability decreased depending on dose and time, and was significantly different in the groups that were treated with 400, 800, and 1,000 µg/mL doses compared to that in the control group (p<0.001). Increasing the dose significantly increased apoptosis (p<0.001). NO secretion from MCF7 cells significantly decreased in response to different concentrations of the extract in a dose- and time-dependent manner (p<0.050).

Conclusion: The crab shell extract inhibited the proliferation of MCF7 cells by increasing apoptosis and decreasing NO production.

No MeSH data available.


Related in: MedlinePlus