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siRNA-Mediated Suppression of Synuclein γ Inhibits MDA-MB-231 Cell Migration and Proliferation by Downregulating the Phosphorylation of AKT and ERK.

He J, Xie N, Yang J, Guan H, Chen W, Wu H, Yuan Z, Wang K, Li G, Sun J, Yu L - J Breast Cancer (2014)

Bottom Line: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA.Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested.Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, China.

ABSTRACT

Purpose: Synuclein-γ (SNCG), which was initially identified as breast cancer specific gene 1, is highly expressed in advanced breast cancers, but not in normal or benign breast tissue. This study aimed to evaluate the effects of SNCG siRNA-treatment on breast cancer cells and elucidate the associated mechanisms.

Methods: Vectors containing SNCG and negative control (NC) siRNAs were transfected into MDA-MB-231 cells; mRNA levels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migration was assessed by the Transwell assay, apoptosis and cell cycle analyses were conducted with the flow cytometer, and Western blot analysis was performed to determine the relative levels of AKT, ERK, p-AKT, and p-ERK expression.

Results: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA. Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested. Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups.

Conclusion: SNCG siRNA could decrease the migration and proliferation of breast cancer cells by downregulating the phosphorylation of AKT and ERK.

No MeSH data available.


Related in: MedlinePlus

Apoptosis of MDA-MB-231 in each group. Synuclein-γ (SNCG) siRNA induced apoptosis in MDA-MB-231 cells. (A) Control-1 group; (B) control-2 group; (C) negative control (NC)-1 group; (D) NC-2 group; (E) SNCG siRNA-1 group; (F) SNCG siRNA-2 group.PI=propidium iodide.
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Figure 4: Apoptosis of MDA-MB-231 in each group. Synuclein-γ (SNCG) siRNA induced apoptosis in MDA-MB-231 cells. (A) Control-1 group; (B) control-2 group; (C) negative control (NC)-1 group; (D) NC-2 group; (E) SNCG siRNA-1 group; (F) SNCG siRNA-2 group.PI=propidium iodide.

Mentions: The rate of apoptosis for MDA-MB-231 cells was analyzed by fluorescence-activated cell sorting (FACS) (Beckmann Coulter Inc., Fullerton, USA). As shown in Figure 4, 26.9% cells in the SNCG siRNA-1 group and 33.2% cells in the SNCG siRNA-2 group were annexin V/FITC-positive, much higher than in the NC1/2 and control 1/2 groups. These results suggest that the SNCG siRNA decreased the proliferation of breast cancer cells through the induction of apoptosis.


siRNA-Mediated Suppression of Synuclein γ Inhibits MDA-MB-231 Cell Migration and Proliferation by Downregulating the Phosphorylation of AKT and ERK.

He J, Xie N, Yang J, Guan H, Chen W, Wu H, Yuan Z, Wang K, Li G, Sun J, Yu L - J Breast Cancer (2014)

Apoptosis of MDA-MB-231 in each group. Synuclein-γ (SNCG) siRNA induced apoptosis in MDA-MB-231 cells. (A) Control-1 group; (B) control-2 group; (C) negative control (NC)-1 group; (D) NC-2 group; (E) SNCG siRNA-1 group; (F) SNCG siRNA-2 group.PI=propidium iodide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197349&req=5

Figure 4: Apoptosis of MDA-MB-231 in each group. Synuclein-γ (SNCG) siRNA induced apoptosis in MDA-MB-231 cells. (A) Control-1 group; (B) control-2 group; (C) negative control (NC)-1 group; (D) NC-2 group; (E) SNCG siRNA-1 group; (F) SNCG siRNA-2 group.PI=propidium iodide.
Mentions: The rate of apoptosis for MDA-MB-231 cells was analyzed by fluorescence-activated cell sorting (FACS) (Beckmann Coulter Inc., Fullerton, USA). As shown in Figure 4, 26.9% cells in the SNCG siRNA-1 group and 33.2% cells in the SNCG siRNA-2 group were annexin V/FITC-positive, much higher than in the NC1/2 and control 1/2 groups. These results suggest that the SNCG siRNA decreased the proliferation of breast cancer cells through the induction of apoptosis.

Bottom Line: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA.Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested.Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, China.

ABSTRACT

Purpose: Synuclein-γ (SNCG), which was initially identified as breast cancer specific gene 1, is highly expressed in advanced breast cancers, but not in normal or benign breast tissue. This study aimed to evaluate the effects of SNCG siRNA-treatment on breast cancer cells and elucidate the associated mechanisms.

Methods: Vectors containing SNCG and negative control (NC) siRNAs were transfected into MDA-MB-231 cells; mRNA levels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migration was assessed by the Transwell assay, apoptosis and cell cycle analyses were conducted with the flow cytometer, and Western blot analysis was performed to determine the relative levels of AKT, ERK, p-AKT, and p-ERK expression.

Results: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA. Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested. Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups.

Conclusion: SNCG siRNA could decrease the migration and proliferation of breast cancer cells by downregulating the phosphorylation of AKT and ERK.

No MeSH data available.


Related in: MedlinePlus