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siRNA-Mediated Suppression of Synuclein γ Inhibits MDA-MB-231 Cell Migration and Proliferation by Downregulating the Phosphorylation of AKT and ERK.

He J, Xie N, Yang J, Guan H, Chen W, Wu H, Yuan Z, Wang K, Li G, Sun J, Yu L - J Breast Cancer (2014)

Bottom Line: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA.Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested.Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, China.

ABSTRACT

Purpose: Synuclein-γ (SNCG), which was initially identified as breast cancer specific gene 1, is highly expressed in advanced breast cancers, but not in normal or benign breast tissue. This study aimed to evaluate the effects of SNCG siRNA-treatment on breast cancer cells and elucidate the associated mechanisms.

Methods: Vectors containing SNCG and negative control (NC) siRNAs were transfected into MDA-MB-231 cells; mRNA levels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migration was assessed by the Transwell assay, apoptosis and cell cycle analyses were conducted with the flow cytometer, and Western blot analysis was performed to determine the relative levels of AKT, ERK, p-AKT, and p-ERK expression.

Results: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA. Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested. Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups.

Conclusion: SNCG siRNA could decrease the migration and proliferation of breast cancer cells by downregulating the phosphorylation of AKT and ERK.

No MeSH data available.


Related in: MedlinePlus

The relative concentration of breast cancer specific gene 1 mRNA in each group. Synuclein-γ (SNCG) and β-actin mRNA levels were determined by fluorescent quantitative polymerase chain reaction. Levels of SNCG mRNA were found to be much lower in SNCG siRNA-1 and SNCG siRNA-2 groups than negative control (NC) (p<0.01, respectively) and control groups (p<0.01, respectively).*p<0.01 vs. control group; †p<0.01 vs. NC group.
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Figure 1: The relative concentration of breast cancer specific gene 1 mRNA in each group. Synuclein-γ (SNCG) and β-actin mRNA levels were determined by fluorescent quantitative polymerase chain reaction. Levels of SNCG mRNA were found to be much lower in SNCG siRNA-1 and SNCG siRNA-2 groups than negative control (NC) (p<0.01, respectively) and control groups (p<0.01, respectively).*p<0.01 vs. control group; †p<0.01 vs. NC group.

Mentions: SNCG and β-actin mRNA levels were determined using fluorescent quantitative PCR; the relative concentrations of SNCG mRNA were calculated (SNCG mRNA relative concentration=SNCG mRNA concentration/β-actin mRNA concentration). As shown in Figure 1, levels of SNCG mRNA were found to be much lower in groups that received SNCG siRNA-1 and SNCG siRNA-2, than in the NC (p<0.01, p<0.01) and control groups (p<0.01, p<0.01). These results suggest that SNCG siRNA can significantly downregulate the SNCG mRNA levels in breast cancer cells.


siRNA-Mediated Suppression of Synuclein γ Inhibits MDA-MB-231 Cell Migration and Proliferation by Downregulating the Phosphorylation of AKT and ERK.

He J, Xie N, Yang J, Guan H, Chen W, Wu H, Yuan Z, Wang K, Li G, Sun J, Yu L - J Breast Cancer (2014)

The relative concentration of breast cancer specific gene 1 mRNA in each group. Synuclein-γ (SNCG) and β-actin mRNA levels were determined by fluorescent quantitative polymerase chain reaction. Levels of SNCG mRNA were found to be much lower in SNCG siRNA-1 and SNCG siRNA-2 groups than negative control (NC) (p<0.01, respectively) and control groups (p<0.01, respectively).*p<0.01 vs. control group; †p<0.01 vs. NC group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4197349&req=5

Figure 1: The relative concentration of breast cancer specific gene 1 mRNA in each group. Synuclein-γ (SNCG) and β-actin mRNA levels were determined by fluorescent quantitative polymerase chain reaction. Levels of SNCG mRNA were found to be much lower in SNCG siRNA-1 and SNCG siRNA-2 groups than negative control (NC) (p<0.01, respectively) and control groups (p<0.01, respectively).*p<0.01 vs. control group; †p<0.01 vs. NC group.
Mentions: SNCG and β-actin mRNA levels were determined using fluorescent quantitative PCR; the relative concentrations of SNCG mRNA were calculated (SNCG mRNA relative concentration=SNCG mRNA concentration/β-actin mRNA concentration). As shown in Figure 1, levels of SNCG mRNA were found to be much lower in groups that received SNCG siRNA-1 and SNCG siRNA-2, than in the NC (p<0.01, p<0.01) and control groups (p<0.01, p<0.01). These results suggest that SNCG siRNA can significantly downregulate the SNCG mRNA levels in breast cancer cells.

Bottom Line: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA.Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested.Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, China.

ABSTRACT

Purpose: Synuclein-γ (SNCG), which was initially identified as breast cancer specific gene 1, is highly expressed in advanced breast cancers, but not in normal or benign breast tissue. This study aimed to evaluate the effects of SNCG siRNA-treatment on breast cancer cells and elucidate the associated mechanisms.

Methods: Vectors containing SNCG and negative control (NC) siRNAs were transfected into MDA-MB-231 cells; mRNA levels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migration was assessed by the Transwell assay, apoptosis and cell cycle analyses were conducted with the flow cytometer, and Western blot analysis was performed to determine the relative levels of AKT, ERK, p-AKT, and p-ERK expression.

Results: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA. Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested. Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups.

Conclusion: SNCG siRNA could decrease the migration and proliferation of breast cancer cells by downregulating the phosphorylation of AKT and ERK.

No MeSH data available.


Related in: MedlinePlus