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The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model.

Chen D, Yang Z, Chen X, Huang Y, Yin B, Guo F, Zhao H, Zhao T, Qu H, Huang J, Wu Y, Gu R - BMC Complement Altern Med (2014)

Bottom Line: Administration of L. rhamnosus hsryfm 1301 or its fermented milk for 28 d, could recover the Bacteroidetes and Verrucomicrobia abundance and could decrease the Firmicutes abundance, which was associated with a significant reduction in the serum lipids' level in the hyperlipidemic rats with high-fat diet induced.The abundance of 22 genera of gut bacteria was changed significantly after probiotic intervention for 28 d (P < 0.05).The Butyrivibrio spp. negatively correlated with TC and LDL-C.

View Article: PubMed Central - PubMed

Affiliation: College of Food Science and Technology, Yangzhou University, Yangzhou 225127, Jiangsu Province, China. guruixia1963@163.com.

ABSTRACT

Background: Growing evidence indicates that intestinal microbiota regulate our metabolism. Probiotics confer health benefits that may depend on their ability to affect the gut microbiota. The objective of this study was to examine the effect of supplementation with the probiotic strain, Lactobacillus rhamnosus hsryfm 1301, on the gut microbiota in a hyperlipidemic rat model, and to explore the associations between the gut microbiota and the serum lipids.

Methods: The hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 d. The rats' gut microbiota were analyzed using high-throughput sequencing before and after L. rhamnosus hsryfm 1301 supplementation or its fermented milk for 28 d. The serum lipids level was also tested.

Results: The rats' primary gut microbiota were composed of Bacteroidetes, Firmicutes, Proteobacteria, Spirochaetes and Verrucomicrobia. The abundance and diversity of the gut microbiota generally decreased after feeding with a high-fat diet, with a significant decrease in the relative abundance of Bacteroidetes, but with an increase in that of Firmicutes (P < 0.05). Administration of L. rhamnosus hsryfm 1301 or its fermented milk for 28 d, could recover the Bacteroidetes and Verrucomicrobia abundance and could decrease the Firmicutes abundance, which was associated with a significant reduction in the serum lipids' level in the hyperlipidemic rats with high-fat diet induced. The abundance of 22 genera of gut bacteria was changed significantly after probiotic intervention for 28 d (P < 0.05). A positive correlation was observed between Ruminococcus spp. and serum triglycerides, Dorea spp. and serum cholesterol (TC) and low-density lipoprotein (LDL-C), and Enterococcus spp. and high-density lipoprotein. The Butyrivibrio spp. negatively correlated with TC and LDL-C.

Conclusions: Our results suggest that the lipid metabolism of hyperlipidemic rats was improved by regulating the gut microbiota with supplementation of L.rhamnosus hsryfm 1301 or its fermented milk for 28 d.

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Related in: MedlinePlus

Physical indexes of rats. Control group: normal diet; model group: high-fat diet; hsryfm 1301 group: high-fat diet + L. rhamnosus hsryfm 1301-containing skim milk suspension; hsryfm 1301-f group: high-fat diet + L. rhamnosus hsryfm 1301-containing skim fermented milk. A: Weight gain of the rats in 56 d; B: Food consumption of the rats in 56 d; C: Efficiency of diet utilization of the rats; D: Serum TC, TG, HDL-C and LDL-C concentration in the rats. Efficiency of diet utilization (%) = (Weight gain/Food consumption) × 100. Each value is the mean ± standard deviation (n = 8). A different superscript letters means significant difference in the same index (P < 0.05).
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Fig2: Physical indexes of rats. Control group: normal diet; model group: high-fat diet; hsryfm 1301 group: high-fat diet + L. rhamnosus hsryfm 1301-containing skim milk suspension; hsryfm 1301-f group: high-fat diet + L. rhamnosus hsryfm 1301-containing skim fermented milk. A: Weight gain of the rats in 56 d; B: Food consumption of the rats in 56 d; C: Efficiency of diet utilization of the rats; D: Serum TC, TG, HDL-C and LDL-C concentration in the rats. Efficiency of diet utilization (%) = (Weight gain/Food consumption) × 100. Each value is the mean ± standard deviation (n = 8). A different superscript letters means significant difference in the same index (P < 0.05).

Mentions: The rats’ body weight increased during the study period. At the end of the experiment, the gained body weight and the average food consumption of the model group were significantly higher than those of the other groups (P < 0.05; Figure 2A and B). The efficiency of diet utilization of the hsryfm 1301-f group was significantly higher than that of the control and model groups (P < 0.05; Figure 2C), indicating that the L. rhamnosus hsryfm fermented milk improved the efficiency of diet utilization.Figure 2


The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model.

Chen D, Yang Z, Chen X, Huang Y, Yin B, Guo F, Zhao H, Zhao T, Qu H, Huang J, Wu Y, Gu R - BMC Complement Altern Med (2014)

Physical indexes of rats. Control group: normal diet; model group: high-fat diet; hsryfm 1301 group: high-fat diet + L. rhamnosus hsryfm 1301-containing skim milk suspension; hsryfm 1301-f group: high-fat diet + L. rhamnosus hsryfm 1301-containing skim fermented milk. A: Weight gain of the rats in 56 d; B: Food consumption of the rats in 56 d; C: Efficiency of diet utilization of the rats; D: Serum TC, TG, HDL-C and LDL-C concentration in the rats. Efficiency of diet utilization (%) = (Weight gain/Food consumption) × 100. Each value is the mean ± standard deviation (n = 8). A different superscript letters means significant difference in the same index (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197344&req=5

Fig2: Physical indexes of rats. Control group: normal diet; model group: high-fat diet; hsryfm 1301 group: high-fat diet + L. rhamnosus hsryfm 1301-containing skim milk suspension; hsryfm 1301-f group: high-fat diet + L. rhamnosus hsryfm 1301-containing skim fermented milk. A: Weight gain of the rats in 56 d; B: Food consumption of the rats in 56 d; C: Efficiency of diet utilization of the rats; D: Serum TC, TG, HDL-C and LDL-C concentration in the rats. Efficiency of diet utilization (%) = (Weight gain/Food consumption) × 100. Each value is the mean ± standard deviation (n = 8). A different superscript letters means significant difference in the same index (P < 0.05).
Mentions: The rats’ body weight increased during the study period. At the end of the experiment, the gained body weight and the average food consumption of the model group were significantly higher than those of the other groups (P < 0.05; Figure 2A and B). The efficiency of diet utilization of the hsryfm 1301-f group was significantly higher than that of the control and model groups (P < 0.05; Figure 2C), indicating that the L. rhamnosus hsryfm fermented milk improved the efficiency of diet utilization.Figure 2

Bottom Line: Administration of L. rhamnosus hsryfm 1301 or its fermented milk for 28 d, could recover the Bacteroidetes and Verrucomicrobia abundance and could decrease the Firmicutes abundance, which was associated with a significant reduction in the serum lipids' level in the hyperlipidemic rats with high-fat diet induced.The abundance of 22 genera of gut bacteria was changed significantly after probiotic intervention for 28 d (P < 0.05).The Butyrivibrio spp. negatively correlated with TC and LDL-C.

View Article: PubMed Central - PubMed

Affiliation: College of Food Science and Technology, Yangzhou University, Yangzhou 225127, Jiangsu Province, China. guruixia1963@163.com.

ABSTRACT

Background: Growing evidence indicates that intestinal microbiota regulate our metabolism. Probiotics confer health benefits that may depend on their ability to affect the gut microbiota. The objective of this study was to examine the effect of supplementation with the probiotic strain, Lactobacillus rhamnosus hsryfm 1301, on the gut microbiota in a hyperlipidemic rat model, and to explore the associations between the gut microbiota and the serum lipids.

Methods: The hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 d. The rats' gut microbiota were analyzed using high-throughput sequencing before and after L. rhamnosus hsryfm 1301 supplementation or its fermented milk for 28 d. The serum lipids level was also tested.

Results: The rats' primary gut microbiota were composed of Bacteroidetes, Firmicutes, Proteobacteria, Spirochaetes and Verrucomicrobia. The abundance and diversity of the gut microbiota generally decreased after feeding with a high-fat diet, with a significant decrease in the relative abundance of Bacteroidetes, but with an increase in that of Firmicutes (P < 0.05). Administration of L. rhamnosus hsryfm 1301 or its fermented milk for 28 d, could recover the Bacteroidetes and Verrucomicrobia abundance and could decrease the Firmicutes abundance, which was associated with a significant reduction in the serum lipids' level in the hyperlipidemic rats with high-fat diet induced. The abundance of 22 genera of gut bacteria was changed significantly after probiotic intervention for 28 d (P < 0.05). A positive correlation was observed between Ruminococcus spp. and serum triglycerides, Dorea spp. and serum cholesterol (TC) and low-density lipoprotein (LDL-C), and Enterococcus spp. and high-density lipoprotein. The Butyrivibrio spp. negatively correlated with TC and LDL-C.

Conclusions: Our results suggest that the lipid metabolism of hyperlipidemic rats was improved by regulating the gut microbiota with supplementation of L.rhamnosus hsryfm 1301 or its fermented milk for 28 d.

Show MeSH
Related in: MedlinePlus