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Microbial contamination of toothbrushes during treatment with multibracket appliances.

Eichenauer J, von Bremen J, Ruf S - Head Face Med (2014)

Bottom Line: Regarding the retention of microorganisms, no differences could be detected between the two bristle designs.However, the amount of S. mutans was significantly higher on brushes used by MB-patients (p < 0.005) than on the brushes of subjects without MB.The number of Lactobacilli and C. albicans was minimal in all cases and below statistical evaluation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthodontics, Justus-Liebig-University, Schlangenzahl 14, 35392 Giessen, Germany. Julia.v.Bremen@dentist.med.uni-giessen.de.

ABSTRACT

Introduction: It was aimed to assess the retention of caries-associated microorganisms on two different manual toothbrushes (conventional and tapered) and to evaluate the influence of multibracket appliances (MB) on the microbial contamination of the brush head.

Methods: 50 MB-patients and 50 subjects without MB received a toothbrush (elmex® interX medium short head or meridol®) plus toothpaste (elmex®) for exclusive use and an information sheet with standardised brushing instructions. After 14 days of regular tooth brushing, the brushes were collected and sluiced in Sputasol solution. The suspension was incubated on selective agar plates and the amount of Streptococcus mutans, lactobacilli and Candida albicans for each brush head was assessed.

Results: Regarding the retention of microorganisms, no differences could be detected between the two bristle designs. However, the amount of S. mutans was significantly higher on brushes used by MB-patients (p < 0.005) than on the brushes of subjects without MB. The number of Lactobacilli and C. albicans was minimal in all cases and below statistical evaluation.

Conclusions: During treatment with MB appliances, toothbrushes were contaminated more intensely with S. mutans independent of bristle design. A more frequent replacement of toothbrushes may thus be recommended for patients undergoing MB-treatment.

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Related in: MedlinePlus

Microbial colonisation with S. mutans in colony forming units (CFU) in patients with and without multibracket appliances using two types of toothbrushes.
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Fig2: Microbial colonisation with S. mutans in colony forming units (CFU) in patients with and without multibracket appliances using two types of toothbrushes.

Mentions: 84% of the toothbrushes showed colonisation with S. mutans, but no difference was found between the two bristle designs (p = 0.6655). However, subjects with MB had significantly higher bacterial counts, independent of brush type (p = 0.0003) than subjects without MB (Figure 2). The highest percentage of contaminated brushes (96%) was found in group MBe, the lowest in group nMBm (70%) (Table 4). Regarding median numbers, subjects without MB harboured 200 (nMBm) and 300 (nMBe) CFU, whereas subjects with MB exhibited 700 CFU (MBm and MBe), respectively (Table 5 and Figure 3). Thus, multibracket appliances appear to enhance the retention of microorganisms on manual toothbrushes significantly. Regarding the mean values, the greatest difference was found between nMBe (400 CFU) and MBe (2322 CFU) (Figure 3). A growth of lactobacilli was observed on only 4 brushes and Candida could not be found on any brush. Hence no statistical analysis could be performed for these two microorganisms.Participants with fixed appliances reported an increased subjective bleeding tendency (p = 0.0065), which didn´t seem to be influenced by a certain toothbrush (p = 0.6018). The perception of pain could neither be related to orthodontic appliances (p = 0.6544) nor to a certain bristle design (p = 0.8639), as was the case when asking for the perceived cleaning efficacy (p = 0.3036 and p = 0.6975) (Figure 4) in all groups.Figure 2


Microbial contamination of toothbrushes during treatment with multibracket appliances.

Eichenauer J, von Bremen J, Ruf S - Head Face Med (2014)

Microbial colonisation with S. mutans in colony forming units (CFU) in patients with and without multibracket appliances using two types of toothbrushes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197314&req=5

Fig2: Microbial colonisation with S. mutans in colony forming units (CFU) in patients with and without multibracket appliances using two types of toothbrushes.
Mentions: 84% of the toothbrushes showed colonisation with S. mutans, but no difference was found between the two bristle designs (p = 0.6655). However, subjects with MB had significantly higher bacterial counts, independent of brush type (p = 0.0003) than subjects without MB (Figure 2). The highest percentage of contaminated brushes (96%) was found in group MBe, the lowest in group nMBm (70%) (Table 4). Regarding median numbers, subjects without MB harboured 200 (nMBm) and 300 (nMBe) CFU, whereas subjects with MB exhibited 700 CFU (MBm and MBe), respectively (Table 5 and Figure 3). Thus, multibracket appliances appear to enhance the retention of microorganisms on manual toothbrushes significantly. Regarding the mean values, the greatest difference was found between nMBe (400 CFU) and MBe (2322 CFU) (Figure 3). A growth of lactobacilli was observed on only 4 brushes and Candida could not be found on any brush. Hence no statistical analysis could be performed for these two microorganisms.Participants with fixed appliances reported an increased subjective bleeding tendency (p = 0.0065), which didn´t seem to be influenced by a certain toothbrush (p = 0.6018). The perception of pain could neither be related to orthodontic appliances (p = 0.6544) nor to a certain bristle design (p = 0.8639), as was the case when asking for the perceived cleaning efficacy (p = 0.3036 and p = 0.6975) (Figure 4) in all groups.Figure 2

Bottom Line: Regarding the retention of microorganisms, no differences could be detected between the two bristle designs.However, the amount of S. mutans was significantly higher on brushes used by MB-patients (p < 0.005) than on the brushes of subjects without MB.The number of Lactobacilli and C. albicans was minimal in all cases and below statistical evaluation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthodontics, Justus-Liebig-University, Schlangenzahl 14, 35392 Giessen, Germany. Julia.v.Bremen@dentist.med.uni-giessen.de.

ABSTRACT

Introduction: It was aimed to assess the retention of caries-associated microorganisms on two different manual toothbrushes (conventional and tapered) and to evaluate the influence of multibracket appliances (MB) on the microbial contamination of the brush head.

Methods: 50 MB-patients and 50 subjects without MB received a toothbrush (elmex® interX medium short head or meridol®) plus toothpaste (elmex®) for exclusive use and an information sheet with standardised brushing instructions. After 14 days of regular tooth brushing, the brushes were collected and sluiced in Sputasol solution. The suspension was incubated on selective agar plates and the amount of Streptococcus mutans, lactobacilli and Candida albicans for each brush head was assessed.

Results: Regarding the retention of microorganisms, no differences could be detected between the two bristle designs. However, the amount of S. mutans was significantly higher on brushes used by MB-patients (p < 0.005) than on the brushes of subjects without MB. The number of Lactobacilli and C. albicans was minimal in all cases and below statistical evaluation.

Conclusions: During treatment with MB appliances, toothbrushes were contaminated more intensely with S. mutans independent of bristle design. A more frequent replacement of toothbrushes may thus be recommended for patients undergoing MB-treatment.

Show MeSH
Related in: MedlinePlus