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Acute oral toxicity of Insampaedok-san, a traditional herbal formula, in rats and its protective effects against ovalbumin-induced asthma via anti-inflammatory and antioxidant properties.

Kim Y, Lee MY, Kim OS, Jeon WY, Shin HK - BMC Complement Altern Med (2014)

Bottom Line: No adverse effects were observed in the acute ISSE toxicity study.ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon 305-811, Republic of Korea. cozy37@gmail.com.

ABSTRACT

Background: Insampaedok-san (ren-shen-bai-du-san in Chinese) is a traditional herbal formula widely used for the treatment of respiratory diseases in Korea and China. In this study, we investigated the acute oral toxicity of an Insampaedok-san water extract (ISSE) in rats and the antiasthmatic effects of ISSE and its mechanism in a model of asthma induced by ovalbumin (OVA) in mice.

Methods: In a safety study, ISSE was administrated orally to rats of both sexes at single doses of 0 and 5000 mg/kg. We observed body weight changes, mortality, clinical signs, and gross pathological findings. In vitro antioxidant activity of ISSE was measured using 2,2-diphenyl-2-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical scavenging methods. A model of asthma was established in mice by sensitization and challenge with OVA. We assessed the levels of type 2 T-helper cytokines, chemokines, and immunoglobulin levels, using enzyme-linked immunosorbent assays, and superoxide dismutase (SOD) activity using a kit.

Results: No adverse effects were observed in the acute ISSE toxicity study. ISSE showed potent free radical scavenging activity and inhibited the recruitment of inflammatory cells into the lung and mucus hypersecretion in OVA-challenged mice. ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.

Conclusions: These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

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Related in: MedlinePlus

Effects of ISSE on IL-4, IL-5, and eotaxin levels in BALF. IL-4 (A), IL-5 (B), and eotaxin (C) levels were measured using an ELISA. NC, negative control (PBS only); OVA, OVA-sensitized/challenged mice; Mon, montelukast (30 mg/kg) + OVA-sensitized/challenged mice; ISSE-100, ISEE (100 mg/kg) + OVA-sensitized/challenged mice; ISSE-200, ISEE (200 mg/kg) + OVA-sensitized/challenged mice. Significantly different from NC, #P < 0.05, ##P < 0.01; significantly different from OVA, *P < 0.05, **P < 0.01.
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Fig4: Effects of ISSE on IL-4, IL-5, and eotaxin levels in BALF. IL-4 (A), IL-5 (B), and eotaxin (C) levels were measured using an ELISA. NC, negative control (PBS only); OVA, OVA-sensitized/challenged mice; Mon, montelukast (30 mg/kg) + OVA-sensitized/challenged mice; ISSE-100, ISEE (100 mg/kg) + OVA-sensitized/challenged mice; ISSE-200, ISEE (200 mg/kg) + OVA-sensitized/challenged mice. Significantly different from NC, #P < 0.05, ##P < 0.01; significantly different from OVA, *P < 0.05, **P < 0.01.

Mentions: To investigate whether ISSE affects Th2 cytokine and chemokine secretion, we measured IL-4, IL-5, and eotaxin levels in the BALF of mice. As shown in Figure 4, OVA challenge induced a significant increase in the levels of IL-4 and IL-5 in BALF. However, the levels of IL-4 and IL-5 were decreased in ISSE-treated mice. Similar to the cytokine levels, eotaxin levels increased in the BALF of OVA-challenged mice compared with those in PBS-treated mice, while the levels of eotaxin were decreased in the ISSE-treated group.Figure 4


Acute oral toxicity of Insampaedok-san, a traditional herbal formula, in rats and its protective effects against ovalbumin-induced asthma via anti-inflammatory and antioxidant properties.

Kim Y, Lee MY, Kim OS, Jeon WY, Shin HK - BMC Complement Altern Med (2014)

Effects of ISSE on IL-4, IL-5, and eotaxin levels in BALF. IL-4 (A), IL-5 (B), and eotaxin (C) levels were measured using an ELISA. NC, negative control (PBS only); OVA, OVA-sensitized/challenged mice; Mon, montelukast (30 mg/kg) + OVA-sensitized/challenged mice; ISSE-100, ISEE (100 mg/kg) + OVA-sensitized/challenged mice; ISSE-200, ISEE (200 mg/kg) + OVA-sensitized/challenged mice. Significantly different from NC, #P < 0.05, ##P < 0.01; significantly different from OVA, *P < 0.05, **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197307&req=5

Fig4: Effects of ISSE on IL-4, IL-5, and eotaxin levels in BALF. IL-4 (A), IL-5 (B), and eotaxin (C) levels were measured using an ELISA. NC, negative control (PBS only); OVA, OVA-sensitized/challenged mice; Mon, montelukast (30 mg/kg) + OVA-sensitized/challenged mice; ISSE-100, ISEE (100 mg/kg) + OVA-sensitized/challenged mice; ISSE-200, ISEE (200 mg/kg) + OVA-sensitized/challenged mice. Significantly different from NC, #P < 0.05, ##P < 0.01; significantly different from OVA, *P < 0.05, **P < 0.01.
Mentions: To investigate whether ISSE affects Th2 cytokine and chemokine secretion, we measured IL-4, IL-5, and eotaxin levels in the BALF of mice. As shown in Figure 4, OVA challenge induced a significant increase in the levels of IL-4 and IL-5 in BALF. However, the levels of IL-4 and IL-5 were decreased in ISSE-treated mice. Similar to the cytokine levels, eotaxin levels increased in the BALF of OVA-challenged mice compared with those in PBS-treated mice, while the levels of eotaxin were decreased in the ISSE-treated group.Figure 4

Bottom Line: No adverse effects were observed in the acute ISSE toxicity study.ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon 305-811, Republic of Korea. cozy37@gmail.com.

ABSTRACT

Background: Insampaedok-san (ren-shen-bai-du-san in Chinese) is a traditional herbal formula widely used for the treatment of respiratory diseases in Korea and China. In this study, we investigated the acute oral toxicity of an Insampaedok-san water extract (ISSE) in rats and the antiasthmatic effects of ISSE and its mechanism in a model of asthma induced by ovalbumin (OVA) in mice.

Methods: In a safety study, ISSE was administrated orally to rats of both sexes at single doses of 0 and 5000 mg/kg. We observed body weight changes, mortality, clinical signs, and gross pathological findings. In vitro antioxidant activity of ISSE was measured using 2,2-diphenyl-2-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical scavenging methods. A model of asthma was established in mice by sensitization and challenge with OVA. We assessed the levels of type 2 T-helper cytokines, chemokines, and immunoglobulin levels, using enzyme-linked immunosorbent assays, and superoxide dismutase (SOD) activity using a kit.

Results: No adverse effects were observed in the acute ISSE toxicity study. ISSE showed potent free radical scavenging activity and inhibited the recruitment of inflammatory cells into the lung and mucus hypersecretion in OVA-challenged mice. ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.

Conclusions: These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

Show MeSH
Related in: MedlinePlus