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Acute oral toxicity of Insampaedok-san, a traditional herbal formula, in rats and its protective effects against ovalbumin-induced asthma via anti-inflammatory and antioxidant properties.

Kim Y, Lee MY, Kim OS, Jeon WY, Shin HK - BMC Complement Altern Med (2014)

Bottom Line: No adverse effects were observed in the acute ISSE toxicity study.ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon 305-811, Republic of Korea. cozy37@gmail.com.

ABSTRACT

Background: Insampaedok-san (ren-shen-bai-du-san in Chinese) is a traditional herbal formula widely used for the treatment of respiratory diseases in Korea and China. In this study, we investigated the acute oral toxicity of an Insampaedok-san water extract (ISSE) in rats and the antiasthmatic effects of ISSE and its mechanism in a model of asthma induced by ovalbumin (OVA) in mice.

Methods: In a safety study, ISSE was administrated orally to rats of both sexes at single doses of 0 and 5000 mg/kg. We observed body weight changes, mortality, clinical signs, and gross pathological findings. In vitro antioxidant activity of ISSE was measured using 2,2-diphenyl-2-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical scavenging methods. A model of asthma was established in mice by sensitization and challenge with OVA. We assessed the levels of type 2 T-helper cytokines, chemokines, and immunoglobulin levels, using enzyme-linked immunosorbent assays, and superoxide dismutase (SOD) activity using a kit.

Results: No adverse effects were observed in the acute ISSE toxicity study. ISSE showed potent free radical scavenging activity and inhibited the recruitment of inflammatory cells into the lung and mucus hypersecretion in OVA-challenged mice. ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.

Conclusions: These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

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Related in: MedlinePlus

Changes in the body weight of rats treated with ISSE for 15 days. Time course of mean body weight after oral administration of ISSE to male (A) and female (B) rats.
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Fig1: Changes in the body weight of rats treated with ISSE for 15 days. Time course of mean body weight after oral administration of ISSE to male (A) and female (B) rats.

Mentions: No rats died during the 15-day acute toxicity study. There was no significant difference in body weight of either male or female rats between the ISSE-treated group and the control group during the 15-day observation period (Figure 1). No abnormal signs regarding general appearance, behavior, or nervous system were observed in rats from the ISSE-treated group compared with the control group rats. Moreover, we did not detect any clinical signs of adverse effects or any abnormal gross autopsy findings induced by ISSE treatment for 15 days.Figure 1


Acute oral toxicity of Insampaedok-san, a traditional herbal formula, in rats and its protective effects against ovalbumin-induced asthma via anti-inflammatory and antioxidant properties.

Kim Y, Lee MY, Kim OS, Jeon WY, Shin HK - BMC Complement Altern Med (2014)

Changes in the body weight of rats treated with ISSE for 15 days. Time course of mean body weight after oral administration of ISSE to male (A) and female (B) rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197307&req=5

Fig1: Changes in the body weight of rats treated with ISSE for 15 days. Time course of mean body weight after oral administration of ISSE to male (A) and female (B) rats.
Mentions: No rats died during the 15-day acute toxicity study. There was no significant difference in body weight of either male or female rats between the ISSE-treated group and the control group during the 15-day observation period (Figure 1). No abnormal signs regarding general appearance, behavior, or nervous system were observed in rats from the ISSE-treated group compared with the control group rats. Moreover, we did not detect any clinical signs of adverse effects or any abnormal gross autopsy findings induced by ISSE treatment for 15 days.Figure 1

Bottom Line: No adverse effects were observed in the acute ISSE toxicity study.ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon 305-811, Republic of Korea. cozy37@gmail.com.

ABSTRACT

Background: Insampaedok-san (ren-shen-bai-du-san in Chinese) is a traditional herbal formula widely used for the treatment of respiratory diseases in Korea and China. In this study, we investigated the acute oral toxicity of an Insampaedok-san water extract (ISSE) in rats and the antiasthmatic effects of ISSE and its mechanism in a model of asthma induced by ovalbumin (OVA) in mice.

Methods: In a safety study, ISSE was administrated orally to rats of both sexes at single doses of 0 and 5000 mg/kg. We observed body weight changes, mortality, clinical signs, and gross pathological findings. In vitro antioxidant activity of ISSE was measured using 2,2-diphenyl-2-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical scavenging methods. A model of asthma was established in mice by sensitization and challenge with OVA. We assessed the levels of type 2 T-helper cytokines, chemokines, and immunoglobulin levels, using enzyme-linked immunosorbent assays, and superoxide dismutase (SOD) activity using a kit.

Results: No adverse effects were observed in the acute ISSE toxicity study. ISSE showed potent free radical scavenging activity and inhibited the recruitment of inflammatory cells into the lung and mucus hypersecretion in OVA-challenged mice. ISSE significantly decreased levels of interleukin (IL)-4, IL-5, eotaxin, and OVA-specific immunoglobulin (Ig)E, and increased SOD activity.

Conclusions: These results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

Show MeSH
Related in: MedlinePlus