Limits...
Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma.

Shen CC, Kang YH, Yu L, Cui DD, He Y, Yang JL, Gou LT - Biol. Res. (2014)

Bottom Line: In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.The lack of TEX101 in seminoma indicated its potential role in tumor progression.This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.

Results: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101.

Conclusions: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

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Expression analysis of TEX101 in testicular cancer. TEX101 expression in seminoma, yolk sac tumor, embryonal carcinoma, and normal testis tissue were examined using immunohistochemistry. The sections showed no staining of TEX101; however, all collected normal testis tissues possessed strong staining of TEX101, resulting in 100% positive expression.
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Fig4: Expression analysis of TEX101 in testicular cancer. TEX101 expression in seminoma, yolk sac tumor, embryonal carcinoma, and normal testis tissue were examined using immunohistochemistry. The sections showed no staining of TEX101; however, all collected normal testis tissues possessed strong staining of TEX101, resulting in 100% positive expression.

Mentions: Because high expression of TEX101 was detected in testis, we also wanted to know if its expression changed in testicular cancer. Thirty-eight testicular cancer tissues, including 36 samples of seminoma and two samples of yolk sac tumor and embryonal carcinoma, were subjected to immunohistochemistry. The sections showed that none of the testicular cancer tissues showed staining for TEX101, resulting in 100% negative expression (Figure 4). In contrast, all 10 samples of normal testis tissues that were collected in this study were confirmed to possess strong staining of TEX101, resulting in 100% positive expression (Figure 4).Figure 4


Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma.

Shen CC, Kang YH, Yu L, Cui DD, He Y, Yang JL, Gou LT - Biol. Res. (2014)

Expression analysis of TEX101 in testicular cancer. TEX101 expression in seminoma, yolk sac tumor, embryonal carcinoma, and normal testis tissue were examined using immunohistochemistry. The sections showed no staining of TEX101; however, all collected normal testis tissues possessed strong staining of TEX101, resulting in 100% positive expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197282&req=5

Fig4: Expression analysis of TEX101 in testicular cancer. TEX101 expression in seminoma, yolk sac tumor, embryonal carcinoma, and normal testis tissue were examined using immunohistochemistry. The sections showed no staining of TEX101; however, all collected normal testis tissues possessed strong staining of TEX101, resulting in 100% positive expression.
Mentions: Because high expression of TEX101 was detected in testis, we also wanted to know if its expression changed in testicular cancer. Thirty-eight testicular cancer tissues, including 36 samples of seminoma and two samples of yolk sac tumor and embryonal carcinoma, were subjected to immunohistochemistry. The sections showed that none of the testicular cancer tissues showed staining for TEX101, resulting in 100% negative expression (Figure 4). In contrast, all 10 samples of normal testis tissues that were collected in this study were confirmed to possess strong staining of TEX101, resulting in 100% positive expression (Figure 4).Figure 4

Bottom Line: In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.The lack of TEX101 in seminoma indicated its potential role in tumor progression.This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.

Results: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101.

Conclusions: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

Show MeSH
Related in: MedlinePlus