Limits...
Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma.

Shen CC, Kang YH, Yu L, Cui DD, He Y, Yang JL, Gou LT - Biol. Res. (2014)

Bottom Line: In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.The lack of TEX101 in seminoma indicated its potential role in tumor progression.This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.

Results: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101.

Conclusions: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

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Related in: MedlinePlus

Expression characteristics of TEX101 in testis. The tissue localization of TEX101 in testis was investigated through immunohistochemistry. Strong staining of TEX101 was found in seminiferous tubules, but not in the connective tissue of testis (50×). In seminiferous tubules, TEX101 was highly expressed in spermatocytes and spermatids, but not in spermatogonia (400×).
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Fig2: Expression characteristics of TEX101 in testis. The tissue localization of TEX101 in testis was investigated through immunohistochemistry. Strong staining of TEX101 was found in seminiferous tubules, but not in the connective tissue of testis (50×). In seminiferous tubules, TEX101 was highly expressed in spermatocytes and spermatids, but not in spermatogonia (400×).

Mentions: Further detailed analysis revealed the expression characteristics of TEX101 in testis. The high magnification view showed that the connective tissue beyond the seminiferous tubules presented negative staining for TEX101. Although TEX101 presented very strong staining in the seminiferous tubules, the negative staining was also observed in some types of cells (Figure 2). Analysis of the sections showed that TEX101 was highly expressed in spermatocytes and spermatids but relatively expressed at low levels in spermatogonia that were near the outer edge of the seminiferous tubules (Figure 2). It is known that the maturation of male germ cells follows the course from spermatogonia to spermatocytes and finally spermatids. Our finding confirmed TEX101 should be related to the maturation process of germ cells and be considered a biomarker for the later stage of development.Figure 2


Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma.

Shen CC, Kang YH, Yu L, Cui DD, He Y, Yang JL, Gou LT - Biol. Res. (2014)

Expression characteristics of TEX101 in testis. The tissue localization of TEX101 in testis was investigated through immunohistochemistry. Strong staining of TEX101 was found in seminiferous tubules, but not in the connective tissue of testis (50×). In seminiferous tubules, TEX101 was highly expressed in spermatocytes and spermatids, but not in spermatogonia (400×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197282&req=5

Fig2: Expression characteristics of TEX101 in testis. The tissue localization of TEX101 in testis was investigated through immunohistochemistry. Strong staining of TEX101 was found in seminiferous tubules, but not in the connective tissue of testis (50×). In seminiferous tubules, TEX101 was highly expressed in spermatocytes and spermatids, but not in spermatogonia (400×).
Mentions: Further detailed analysis revealed the expression characteristics of TEX101 in testis. The high magnification view showed that the connective tissue beyond the seminiferous tubules presented negative staining for TEX101. Although TEX101 presented very strong staining in the seminiferous tubules, the negative staining was also observed in some types of cells (Figure 2). Analysis of the sections showed that TEX101 was highly expressed in spermatocytes and spermatids but relatively expressed at low levels in spermatogonia that were near the outer edge of the seminiferous tubules (Figure 2). It is known that the maturation of male germ cells follows the course from spermatogonia to spermatocytes and finally spermatids. Our finding confirmed TEX101 should be related to the maturation process of germ cells and be considered a biomarker for the later stage of development.Figure 2

Bottom Line: In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.The lack of TEX101 in seminoma indicated its potential role in tumor progression.This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.

Results: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101.

Conclusions: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

Show MeSH
Related in: MedlinePlus