Limits...
Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma.

Shen CC, Kang YH, Yu L, Cui DD, He Y, Yang JL, Gou LT - Biol. Res. (2014)

Bottom Line: In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.The lack of TEX101 in seminoma indicated its potential role in tumor progression.This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.

Results: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101.

Conclusions: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

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Related in: MedlinePlus

Expression analysis of TEX101 in various human tissues. Immunohistochemistry was performed to examine 28 types of tissues using a rabbit polyclonal antibody against TEX101. These organs covered a wide range, including cardiac muscle, lung, kidney, spleen, liver, esophagus, stomach, small intestine, colon, pancreas, salivary gland, ovary, uterine cervix, endometrium, breast, testis, prostate, hypophysis, thyroid gland, parathyroid gland, adrenal gland, thymus gland, tonsil, larynx, cerebellum, eye, peripheral nerves, and skin. The tissues sections were visualized using diaminobenzidine, and images were captured with a Leica DM2500 microscope (200×). Clear and strong staining was observed in the testis samples, but not in the other tissue samples, including ovary.
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Fig1: Expression analysis of TEX101 in various human tissues. Immunohistochemistry was performed to examine 28 types of tissues using a rabbit polyclonal antibody against TEX101. These organs covered a wide range, including cardiac muscle, lung, kidney, spleen, liver, esophagus, stomach, small intestine, colon, pancreas, salivary gland, ovary, uterine cervix, endometrium, breast, testis, prostate, hypophysis, thyroid gland, parathyroid gland, adrenal gland, thymus gland, tonsil, larynx, cerebellum, eye, peripheral nerves, and skin. The tissues sections were visualized using diaminobenzidine, and images were captured with a Leica DM2500 microscope (200×). Clear and strong staining was observed in the testis samples, but not in the other tissue samples, including ovary.

Mentions: To elucidate the expression profile of TEX101, 28 types of normal human organs were collected and subjected to immunohistochemistry with a high-quality anti-TEX101 antibody. Analysis of the sections showed that testis was the only organ in which TEX101 was highly expressed (Figure 1). The staining for TEX101 was not detected in the other 27 types of organs, including ovary (Figure 1). These results confirmed that TEX101 was a testis-abundant protein. This marker has been utilized to diagnose male infertility [16, 17].Figure 1


Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma.

Shen CC, Kang YH, Yu L, Cui DD, He Y, Yang JL, Gou LT - Biol. Res. (2014)

Expression analysis of TEX101 in various human tissues. Immunohistochemistry was performed to examine 28 types of tissues using a rabbit polyclonal antibody against TEX101. These organs covered a wide range, including cardiac muscle, lung, kidney, spleen, liver, esophagus, stomach, small intestine, colon, pancreas, salivary gland, ovary, uterine cervix, endometrium, breast, testis, prostate, hypophysis, thyroid gland, parathyroid gland, adrenal gland, thymus gland, tonsil, larynx, cerebellum, eye, peripheral nerves, and skin. The tissues sections were visualized using diaminobenzidine, and images were captured with a Leica DM2500 microscope (200×). Clear and strong staining was observed in the testis samples, but not in the other tissue samples, including ovary.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197282&req=5

Fig1: Expression analysis of TEX101 in various human tissues. Immunohistochemistry was performed to examine 28 types of tissues using a rabbit polyclonal antibody against TEX101. These organs covered a wide range, including cardiac muscle, lung, kidney, spleen, liver, esophagus, stomach, small intestine, colon, pancreas, salivary gland, ovary, uterine cervix, endometrium, breast, testis, prostate, hypophysis, thyroid gland, parathyroid gland, adrenal gland, thymus gland, tonsil, larynx, cerebellum, eye, peripheral nerves, and skin. The tissues sections were visualized using diaminobenzidine, and images were captured with a Leica DM2500 microscope (200×). Clear and strong staining was observed in the testis samples, but not in the other tissue samples, including ovary.
Mentions: To elucidate the expression profile of TEX101, 28 types of normal human organs were collected and subjected to immunohistochemistry with a high-quality anti-TEX101 antibody. Analysis of the sections showed that testis was the only organ in which TEX101 was highly expressed (Figure 1). The staining for TEX101 was not detected in the other 27 types of organs, including ovary (Figure 1). These results confirmed that TEX101 was a testis-abundant protein. This marker has been utilized to diagnose male infertility [16, 17].Figure 1

Bottom Line: In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.The lack of TEX101 in seminoma indicated its potential role in tumor progression.This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed.

Results: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101.

Conclusions: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.

Show MeSH
Related in: MedlinePlus