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Evaluation of acute toxicity and gastroprotective activity of curcuma purpurascens BI. rhizome against ethanol-induced gastric mucosal injury in rats.

Rouhollahi E, Moghadamtousi SZ, Hamdi OA, Fadaeinasab M, Hajrezaie M, Awang K, Looi CY, Abdulla MA, Mohamed Z - BMC Complement Altern Med (2014)

Bottom Line: Further examination of gastric mucosal homogenate revealed significant elevation of superoxide dismutase and nitric oxide activities and reduction in malondialdehyde level in CPRHE-treated group, compared to the lesion control group.Immunohistochemical staining showed down-regulation of Bax protein and up-regulation of Hsp70 protein.Taken together, these findings confirmed the gastroprotective effect of Curcuma purpurascens rhizome against gastric damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Pharmacogenomics Laboratory, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. e_rouhollahi@yahoo.com.

ABSTRACT

Background: Curcuma purpurascens BI. is a medicinal plant from the Zingiberaceae family, which is widely used as a spice and as folk medicine. The aim of the present study is to investigate the gastroprotective activity of C. purpurascens rhizome hexane extract (CPRHE) against ethanol- induced gastric ulcers in rats.

Methods: Acute toxicity test was carried out on 36 rats (18 males and 18 females) with low dose of CPRHE (1 g/kg), high dose of CPRHE (2 g/kg) and vehicle (5% Tween 20). To determine the gastroprotective effect of CPRHE, gastric juice acidity, gross and histological gastric lesions, mucus content and ulcer index were evaluated in ethanol-induced ulcer in rats. In addition, superoxide dismutase activity, nitric oxide level and immunohistochemical evaluation of Bax and HSP70 proteins were examined.

Results: The CPRHE acute toxicity test on rats did not reveal any signs of mortality and toxicity up to 2 g/kg. The oral administration of CPRHE at doses of 200 mg/kg and 400 mg/kg and omeprazole (positive control) at a dose of 20 mg/kg to rats remarkably attenuated gastric lesions induced by ethanol. Pre-treatment of rats with CPRHE significantly replenished the depletion of mucus content caused by ethanol administration and decreased the acidity of gastric walls. Further examination of gastric mucosal homogenate revealed significant elevation of superoxide dismutase and nitric oxide activities and reduction in malondialdehyde level in CPRHE-treated group, compared to the lesion control group. Histological assessment of gastric walls obtained from rats pre-treated with CPRHE demonstrated a noteworthy decrease in hemorrhagic mucosal lesions. Immunohistochemical staining showed down-regulation of Bax protein and up-regulation of Hsp70 protein.

Conclusion: Taken together, these findings confirmed the gastroprotective effect of Curcuma purpurascens rhizome against gastric damage.

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Related in: MedlinePlus

Gross evaluation. Results demonstrated that the rodents pre-treated with (C) omeprazole and CPRHE at doses of (D) 200 mg/kg and (E) 400 mg/kg had conspicuously decreased area of gastric ulcer formation compared to (B) ulcer control. (A) Normal control group demonstrated no gastric lesion formation.
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Fig3: Gross evaluation. Results demonstrated that the rodents pre-treated with (C) omeprazole and CPRHE at doses of (D) 200 mg/kg and (E) 400 mg/kg had conspicuously decreased area of gastric ulcer formation compared to (B) ulcer control. (A) Normal control group demonstrated no gastric lesion formation.

Mentions: The administration of ethanol to the rats induced noticeable black hemorrhagic lesions in the gastric walls (Figure 3B) with ulcer area of 880 ± 16.23 (mm)2 (Table 4). As shown in Figure 3, rodents pre-treated with CPRHE (Figure 3D and E) and omeprazole (Figure 3C) had markedly reduced areas of gastric ulcer formation in comparison with lesion control group (Figure 3B). Pre-treatment of rats with CPRHE at doses of 200 mg/kg and 400 mg/kg suppressed the ulcer area formation to 455 ± 12.24 (mm)2 and 325 ± 10.67 (mm)2, which was comparable to the suppressive effect of omeprazole 195 ± 8.97 (mm)2. The incidence of ulcer was decreased by 48% and 63% after treatment with CPRHE at doses of 200 mg/kg and 400 mg/kg, respectively (Table 4). It is worthy to note that CPRHE treatment helped to flatten the gastric mucosal folds in rodents (Figure 3D and E).Figure 3


Evaluation of acute toxicity and gastroprotective activity of curcuma purpurascens BI. rhizome against ethanol-induced gastric mucosal injury in rats.

Rouhollahi E, Moghadamtousi SZ, Hamdi OA, Fadaeinasab M, Hajrezaie M, Awang K, Looi CY, Abdulla MA, Mohamed Z - BMC Complement Altern Med (2014)

Gross evaluation. Results demonstrated that the rodents pre-treated with (C) omeprazole and CPRHE at doses of (D) 200 mg/kg and (E) 400 mg/kg had conspicuously decreased area of gastric ulcer formation compared to (B) ulcer control. (A) Normal control group demonstrated no gastric lesion formation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4197259&req=5

Fig3: Gross evaluation. Results demonstrated that the rodents pre-treated with (C) omeprazole and CPRHE at doses of (D) 200 mg/kg and (E) 400 mg/kg had conspicuously decreased area of gastric ulcer formation compared to (B) ulcer control. (A) Normal control group demonstrated no gastric lesion formation.
Mentions: The administration of ethanol to the rats induced noticeable black hemorrhagic lesions in the gastric walls (Figure 3B) with ulcer area of 880 ± 16.23 (mm)2 (Table 4). As shown in Figure 3, rodents pre-treated with CPRHE (Figure 3D and E) and omeprazole (Figure 3C) had markedly reduced areas of gastric ulcer formation in comparison with lesion control group (Figure 3B). Pre-treatment of rats with CPRHE at doses of 200 mg/kg and 400 mg/kg suppressed the ulcer area formation to 455 ± 12.24 (mm)2 and 325 ± 10.67 (mm)2, which was comparable to the suppressive effect of omeprazole 195 ± 8.97 (mm)2. The incidence of ulcer was decreased by 48% and 63% after treatment with CPRHE at doses of 200 mg/kg and 400 mg/kg, respectively (Table 4). It is worthy to note that CPRHE treatment helped to flatten the gastric mucosal folds in rodents (Figure 3D and E).Figure 3

Bottom Line: Further examination of gastric mucosal homogenate revealed significant elevation of superoxide dismutase and nitric oxide activities and reduction in malondialdehyde level in CPRHE-treated group, compared to the lesion control group.Immunohistochemical staining showed down-regulation of Bax protein and up-regulation of Hsp70 protein.Taken together, these findings confirmed the gastroprotective effect of Curcuma purpurascens rhizome against gastric damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Pharmacogenomics Laboratory, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. e_rouhollahi@yahoo.com.

ABSTRACT

Background: Curcuma purpurascens BI. is a medicinal plant from the Zingiberaceae family, which is widely used as a spice and as folk medicine. The aim of the present study is to investigate the gastroprotective activity of C. purpurascens rhizome hexane extract (CPRHE) against ethanol- induced gastric ulcers in rats.

Methods: Acute toxicity test was carried out on 36 rats (18 males and 18 females) with low dose of CPRHE (1 g/kg), high dose of CPRHE (2 g/kg) and vehicle (5% Tween 20). To determine the gastroprotective effect of CPRHE, gastric juice acidity, gross and histological gastric lesions, mucus content and ulcer index were evaluated in ethanol-induced ulcer in rats. In addition, superoxide dismutase activity, nitric oxide level and immunohistochemical evaluation of Bax and HSP70 proteins were examined.

Results: The CPRHE acute toxicity test on rats did not reveal any signs of mortality and toxicity up to 2 g/kg. The oral administration of CPRHE at doses of 200 mg/kg and 400 mg/kg and omeprazole (positive control) at a dose of 20 mg/kg to rats remarkably attenuated gastric lesions induced by ethanol. Pre-treatment of rats with CPRHE significantly replenished the depletion of mucus content caused by ethanol administration and decreased the acidity of gastric walls. Further examination of gastric mucosal homogenate revealed significant elevation of superoxide dismutase and nitric oxide activities and reduction in malondialdehyde level in CPRHE-treated group, compared to the lesion control group. Histological assessment of gastric walls obtained from rats pre-treated with CPRHE demonstrated a noteworthy decrease in hemorrhagic mucosal lesions. Immunohistochemical staining showed down-regulation of Bax protein and up-regulation of Hsp70 protein.

Conclusion: Taken together, these findings confirmed the gastroprotective effect of Curcuma purpurascens rhizome against gastric damage.

Show MeSH
Related in: MedlinePlus